WB
H
Endogenous
32
Rabbit IgG
#O00757
8789
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human FBP2/FBPase 2 protein.
Background
Fructose-1,6-bisphosphatase 2 (FBP2 or FBPase 2) is a rate limiting enzyme in gluconeogenesis. It catalyzes the conversion of fructose-1,6-bisphosphate to fructose-6-phosphate (1,2). FBP2/FBPase 2 expression is inhibited in various soft tissue sarcoma subtypes. Ectopic expression of FBP2/FBPase 2 represses soft tissue sarcoma cell proliferation and tumor growth. Studies show that cytosolic FBP2/FBPase 2 inhibits glycolysis through its catalytic activity, suppressing sarcoma cell proliferation. In addition, nuclear FBP2/FBPase 2 inhibits mitochondrial biogenesis and oxidative phosphorylation independent of its catalytic activity, also contributing to the suppression of sarcoma cell proliferation (2).
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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