FBP2/FBPase 2 (E9M5T) Rabbit mAb #48159

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Supporting Data

REACTIVITY	H
SENSITIVITY	Endogenous
MW (kDa)	32
Source/Isotype	Rabbit IgG

Application Key:

WB-Western Blotting

Species Cross-Reactivity Key:

H-Human

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

FBP2/FBPase 2 (E9M5T) Rabbit mAb recognizes endogenous levels of total FBP2/FBPase 2 protein. This antibody does not cross-react with FBP1/FBPase 1 protein. This antibody detects an 80 kDa nonspecific band.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human FBP2/FBPase 2 protein.

Background

Fructose-1,6-bisphosphatase 2 (FBP2 or FBPase 2) is a rate limiting enzyme in gluconeogenesis. It catalyzes the conversion of fructose-1,6-bisphosphate to fructose-6-phosphate (1,2). FBP2/FBPase 2 expression is inhibited in various soft tissue sarcoma subtypes. Ectopic expression of FBP2/FBPase 2 represses soft tissue sarcoma cell proliferation and tumor growth. Studies show that cytosolic FBP2/FBPase 2 inhibits glycolysis through its catalytic activity, suppressing sarcoma cell proliferation. In addition, nuclear FBP2/FBPase 2 inhibits mitochondrial biogenesis and oxidative phosphorylation independent of its catalytic activity, also contributing to the suppression of sarcoma cell proliferation (2).

Database Links

UniProt ID: O00757

Entrez-Gene Id: 8789

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For Research Use Only. Not For Use In Diagnostic Procedures.

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