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FBP2/FBPase 2 (E9M5T) Rabbit mAb #48159

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  • WB

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 32
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    FBP2/FBPase 2 (E9M5T) Rabbit mAb recognizes endogenous levels of total FBP2/FBPase 2 protein. This antibody does not cross-react with FBP1/FBPase 1 protein. This antibody detects an 80 kDa nonspecific band.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human FBP2/FBPase 2 protein.

    Background

    Fructose-1,6-bisphosphatase 2 (FBP2 or FBPase 2) is a rate limiting enzyme in gluconeogenesis. It catalyzes the conversion of fructose-1,6-bisphosphate to fructose-6-phosphate (1,2). FBP2/FBPase 2 expression is inhibited in various soft tissue sarcoma subtypes. Ectopic expression of FBP2/FBPase 2 represses soft tissue sarcoma cell proliferation and tumor growth. Studies show that cytosolic FBP2/FBPase 2 inhibits glycolysis through its catalytic activity, suppressing sarcoma cell proliferation. In addition, nuclear FBP2/FBPase 2 inhibits mitochondrial biogenesis and oxidative phosphorylation independent of its catalytic activity, also contributing to the suppression of sarcoma cell proliferation (2).

    Alternate Names

    D-fructose-1,6-bisphosphate 1-phosphohydrolase 2; F16P2; FBP2; FBPase 2; fructose-1,6-bisphosphatase 2; Fructose-1,6-bisphosphatase isozyme 2; fructose-bisphosphatase 2; hexosediphosphatase; muscle FBPase; muscle fructose-bisphosphatase

    For Research Use Only. Not for Use in Diagnostic Procedures.
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