WB, IP
H Mk
Endogenous
140
Rabbit IgG
#Q9P278
57600
Product Information
Product Usage Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
Immunoprecipitation | 1:100 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly26 of human FNIP2 protein.
Background
FNIP1 and FNIP2 were identified as proteins interacting with tumor suppressor folliculin (FLCN) (1-3). FNIP1 and FNIP2 directly associate with AMPK, which indicates that they play roles in the energy and nutrient sensing pathways (1, 2). Further studies show that the FLCN/FNIP2 complex functions as a GTPase-activating protein for Rag GTPase C and Rag GTPase D (4). FLCN/FNIP2 complex thus promotes the binding of mTORC1 to Rag heterodimers, leading to the mTORC1 activation (4). In addition, along with FLCN, both FNIP1 and FNIP2 contribute to kidney tumor suppression (5).
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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