WB
Vir
Endogenous
40
Rabbit
#G8EFI2
11293538
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Specificity / Sensitivity
Species Reactivity:
Virus
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro47 of Lloviu virus VP35 protein. Antibodies are purified by peptide affinity chromatography.
Background
The Lloviu virus (LLOV) is a pathogenic filovirus identified in bat populations in southern Europe (1-3). It belongs to the Filoviridae family, members of which (e.g., Ebola, Marburg) are known to cause fatal hemorrhagic diseases in primates, including humans (4). Transcription and replication of filoviruses requires formation of a ribonucleoprotein (RNP) complex, composed of nucleoprotein (NP) together with viral polymerase enzymes and cofactors. Viral Protein 35 (VP35), a polymerase cofactor, is one of the key components of the RNP complex in filoviruses (5). In addition to its role in promoting viral replication, research studies in both bat and human cells have shown that Lloviu virus VP35 can suppress the host innate immune response to viral infection, in part through suppression of the interferon response (6). These immunosuppressive functions of Lloviu virus VP35 are consistent with those observed for VP35 homologs in other filovirus species (7,8).
- Kuhn, J.H. et al. (2010) Arch Virol 155, 2083-103.
- Negredo, A. et al. (2011) PLoS Pathog 7, e1002304.
- Kemenesi, G. et al. (2022) Nat Commun 13, 1706.
- Barrette, R.W. et al. (2011) Infect Genet Evol 11, 1514-9.
- Feagins, A.R. and Basler, C.F. (2015) Virology 485, 145-52.
- Shu, T. et al. (2019) Nucleic Acids Res 47, 5837-5851.
- Basler, C.F. et al. (2003) J Virol 77, 7945-56.
- Hume, A. and Mühlberger, E. (2018) J Infect Dis 218, S403-S408.
- Hume, A.J. et al. (2022) PLoS Pathog 18, e1010268.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
Limited Uses
Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.
Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.
