WB, IP
H
Endogenous
46, 48
Rabbit
#Q9Y5Q3
9935
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding of human Pro188 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background
MAFB belongs to the musculoaponeurotic fibrosarcoma (MAF) family of basic leucine-zipper transcription factors (1). In mouse embryo, MAFB expression is first detected at E10.5 (2,3). Early in development, MAFB drives differentiation of both glucagon-producing α-cells and insulin-producing β-cells in the pancreas, but later plays a more decisive role in the maturation and maintenance of functional α-cells (4,5). Consistent with MAFB playing a critical role in mature α-cells, MAFB is enriched in α-cells within two weeks of birth in the pancreas (6). Glucagon and insulin secretion is tightly regulated, and imbalances in these hormones contribute to metabolic conditions. Therefore, understanding the role of MAFB in α-cell development, maintenance, and physiological function may contribute to developing deeper insights into how these cells contribute to metabolic diseases like diabetes. MAFB also regulates monocyte differentiation, indicating MAFB functions beyond the pancreas (7).
- Hang, Y. and Stein, R. (2011) Trends Endocrinol Metab 22, 364-73.
- Nishimura, W. et al. (2006) Dev Biol 293, 526-39.
- Artner, I. et al. (2006) Diabetes 55, 297-304.
- Katoh, M.C. et al. (2018) Mol Cell Biol 38, e00504-17.
- Artner, I. et al. (2007) Proc Natl Acad Sci U S A 104, 3853-8.
- Artner, I. et al. (2010) Diabetes 59, 2530-9.
- Wu, X. et al. (2016) J Exp Med 213, 2553-65.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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