Render Target: STATIC
Render Timestamp:
3/25/2025, 7:05:01 AM EDT
3/25/2025, 11:05:01 AM UTC
Commit: 8d93f7ebe45006d66c127727d817fc3f57c4fe9a
XML generation date: 2025-03-07 13:09:27.131
Product last modified at: 2025-03-19T11:30:09.754Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

MAFB Antibody #41019

Filter:
  • WB
  • IP

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 46, 48
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    MAFB Antibody recognizes endogenous levels of total MAFB protein. Based on sequence similarity, this antibody is not predicted to cross-react with MAFA.

    Species Reactivity:

    Human

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding of human Pro188 protein. Antibodies are purified by protein A and peptide affinity chromatography.

    Background

    MAFB belongs to the musculoaponeurotic fibrosarcoma (MAF) family of basic leucine-zipper transcription factors (1). In mouse embryo, MAFB expression is first detected at E10.5 (2,3). Early in development, MAFB drives differentiation of both glucagon-producing α-cells and insulin-producing β-cells in the pancreas, but later plays a more decisive role in the maturation and maintenance of functional α-cells (4,5). Consistent with MAFB playing a critical role in mature α-cells, MAFB is enriched in α-cells within two weeks of birth in the pancreas (6). Glucagon and insulin secretion is tightly regulated, and imbalances in these hormones contribute to metabolic conditions. Therefore, understanding the role of MAFB in α-cell development, maintenance, and physiological function may contribute to developing deeper insights into how these cells contribute to metabolic diseases like diabetes. MAFB also regulates monocyte differentiation, indicating MAFB functions beyond the pancreas (7).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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