WB
Vir
Transfected Only
20-30
Rabbit IgG
#K9N7A1
37616438
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Specificity / Sensitivity
Species Reactivity:
Virus
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln184 of MERS-CoV membrane protein.
Background
Middle East Respiratory Syndrome (MERS) is a contagious viral respiratory infection, whose causative agent was identified as the MERS-related coronavirus (MERS-CoV) (1,2). MERS-CoV is a single-stranded mRNA betacoronavirus; similar to other infectious coronaviruses (e.g., SARS-CoV-2), the MERS-CoV genome encodes four key structural proteins (spike, envelope, membrane, and nucleocapsid) that make up the virion particle, in addition to at least 10 smaller open reading frames (ORFs) that encode the non-structural (accessory) proteins required for viral replication (3). The MERS membrane protein, like that of other betacoronaviruses, is an integral membrane protein and the most abundant of the structural proteins in a virus particle. Numerous studies have shown that the membrane protein of betacoronaviruses is more than just a structural protein; it plays multiple functional roles in orchestrating viral replication following host infection (4-7).
- Bermingham, A. et al. (2012) Euro Surveill 17, 20290.
- de Groot, R.J. et al. (2013) J Virol 87, 7790-2.
- van Boheemen, S. et al. (2012) mBio 3, e00473-12. doi: 10.1128/mBio.00473-12.
- Wong, N.A. and Saier, M.H. (2021) Int J Mol Sci 22, 1308. doi: 10.3390/ijms22031308.
- Neuman, B.W. et al. (2011) J Struct Biol 174, 11-22.
- Schoeman, D. and Fielding, B.C. (2019) Virol J 16, 69.
- J Alsaadi, E.A. and Jones, I.M. (2019) Future Virol 14, 275-286.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
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