WB, IP
H R
Endogenous
45
Rabbit IgG
#Q4G0N4
133686
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val331 of human NADK2 protein.
Background
NAD kinase 2 (NADK2) is a mitochondrial enzyme that catalyzes the phosphorylation of NAD+ to NADP+. This enzyme uses ATP or inorganic polyphosphate as a phosphoryl donor. Besides NAD+, NADK2 can also use NADH as a substrate (1). NADK2 is essential for NADP+ and NADPH generation in mitochondria. Studies show that tricarboxylic acid cycle activity, mitochondrial folate pathway activity, and oxidative stress in mitochondria are not affected when the NADK2 gene is deleted. However, NADK2 deletion leads to diminished NADP+ and NADPH in mitochondria and negatively impacts the reduction of glutamate to pyrroline-5-carboxylate needed for proline biosynthesis. As a result, NADK2 deletion slows the proliferation of cells cultured in minimal medium (2).
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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