NADK2 (F5X1V) Rabbit mAb #82073

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Supporting Data

REACTIVITY	H R
SENSITIVITY	Endogenous
MW (kDa)	45
Source/Isotype	Rabbit IgG

Application Key:

WB-Western Blotting
IP-Immunoprecipitation

Species Cross-Reactivity Key:

H-Human
R-Rat

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000
Simple Western™	1:10 - 1:50
Immunoprecipitation	1:100

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

NADK2 (F5X1V) Rabbit mAb recognizes endogenous levels of total NADK2 protein. This antibody does not cross-react with NADK1 protein.

Species Reactivity:

Human, Rat

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val331 of human NADK2 protein.

Background

NAD kinase 2 (NADK2) is a mitochondrial enzyme that catalyzes the phosphorylation of NAD⁺ to NADP⁺. This enzyme uses ATP or inorganic polyphosphate as a phosphoryl donor. Besides NAD⁺, NADK2 can also use NADH as a substrate (1). NADK2 is essential for NADP⁺ and NADPH generation in mitochondria. Studies show that tricarboxylic acid cycle activity, mitochondrial folate pathway activity, and oxidative stress in mitochondria are not affected when the NADK2 gene is deleted. However, NADK2 deletion leads to diminished NADP⁺ and NADPH in mitochondria and negatively impacts the reduction of glutamate to pyrroline-5-carboxylate needed for proline biosynthesis. As a result, NADK2 deletion slows the proliferation of cells cultured in minimal medium (2).

Database Links

UniProt ID: Q4G0N4

Entrez-Gene Id: 133686

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For Research Use Only. Not For Use In Diagnostic Procedures.

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