WB, IP, eCLIP
H M R Mk
Endogenous
50
Rabbit IgG
#Q86U42
8106
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
| eCLIP | 1:200 |
For more information about the RBP-eCLIP service please visit Eclipsebio.
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat, Monkey
Species predicted to react based on 100% sequence homology
The antigen sequence used to produce this antibody shares
100% sequence homology with the species listed here, but
reactivity has not been tested or confirmed to work by CST.
Use of this product with these species is not covered under
our
Product Performance Guarantee.
Bovine, Rabbit
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human PABP2 protein.
Background
Poly(A)-binding protein 2 (PABP2, PAPBN1) is a nuclear RNA-binding protein involved in the post-transcriptional processing of RNA molecules (1). The PABP2 protein enhances RNA polyadenylation by stimulating poly(A) polymerase (PAP) activity and facilitating the interaction between PAP and the cleavage and specificity factor (CPSF) to regulate poly(A) tail length (2-4). The role that PABP2 plays in regulating poly(A) tail formation and site selection may be important in influencing the length of the 3’ untranslated region (UTR), which can alter transcript stability and translation by RNA binding proteins and miRNAs (1,5). Mutations in the corresponding PABPN1 gene can result in oculopharyngeal muscular dystrophy (OPMD), an autosomal dominant muscle disorder characterized by weakness in proximal limb muscles, ptosis, and dysphagia (1,6).
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation eCLIP: eCLIP
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
Limited Uses
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