PABP2 (D6F3Q) Rabbit mAb #14154
- WB
- IP
- eCLIP
Supporting Data
| REACTIVITY | H M R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 50 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- eCLIP-eCLIP
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
| eCLIP | 1:200 |
Storage
Protocol
Specificity / Sensitivity
PABP2 (D6F3Q) Rabbit mAb recognizes endogenous levels of total PABP2 protein.
Species Reactivity:
Human, Mouse, Rat, Monkey
The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.
Species predicted to react based on 100% sequence homology:
Bovine, Rabbit
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human PABP2 protein.
Background
Poly(A)-binding protein 2 (PABP2, PAPBN1) is a nuclear RNA-binding protein involved in the post-transcriptional processing of RNA molecules (1). The PABP2 protein enhances RNA polyadenylation by stimulating poly(A) polymerase (PAP) activity and facilitating the interaction between PAP and the cleavage and specificity factor (CPSF) to regulate poly(A) tail length (2-4). The role that PABP2 plays in regulating poly(A) tail formation and site selection may be important in influencing the length of the 3’ untranslated region (UTR), which can alter transcript stability and translation by RNA binding proteins and miRNAs (1,5). Mutations in the corresponding PABPN1 gene can result in oculopharyngeal muscular dystrophy (OPMD), an autosomal dominant muscle disorder characterized by weakness in proximal limb muscles, ptosis, and dysphagia (1,6).
Limited Uses
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