Plasma Membrane Marker Antibody Sampler Kit (#64097) Datasheet with Images Cell Signaling Technology

For Research Use Only. Not for Use in Diagnostic Procedures.

Product Includes	Product #	Quantity	Mol. Wt	Isotype/Source
Phospho-Na,K-ATPase α1 (Tyr10) (E1Y9C) Rabbit mAb	13566	20 µl	100 kDa	Rabbit IgG
Pan-Cadherin (28E12) Rabbit mAb	4073	20 µl	130-150 kDa	Rabbit IgG
E-Cadherin (24E10) Rabbit mAb	3195	20 µl	135 kDa	Rabbit IgG
4F2hc/SLC3A2 (D3F9D) XP^® Rabbit mAb	47213	20 µl	75-120 kDa	Rabbit IgG
Caveolin-1 (D46G3) XP^® Rabbit mAb	3267	20 µl	21, 24 kDa	Rabbit IgG
N-Cadherin (D4R1H) XP^® Rabbit mAb	13116	20 µl	140 kDa	Rabbit IgG
ENPP1 (D37B7) Rabbit mAb	5342	20 µl	140 kDa	Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody	7074	100 µl		Goat
Na,K-ATPase α1 (D4Y7E) Rabbit mAb	23565	20 µl	100 kDa	Rabbit IgG

Please visit cellsignal.com for individual component applications, species cross-reactivity, dilutions, protocols, and additional product information.

Description

The Plasma Membrane Marker Antibody Sampler Kit provides an economical means of detecting plasma membrane markers in various cell types. The kit includes enough antibodies to perform two western blot experiments with each primary antibody.

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibodies.

Background

The Na,K-ATPase is an integral membrane heterodimer belonging to the P-type ATPase family. Phosphorylation of Na,K-ATPase at Tyr10 has been implicated in the regulation of enzyme activity in response to hormones and neurotransmitters (1). Cadherins are a superfamily of transmembrane glycoproteins that contain cadherin repeats of approximately 100 residues in their extracellular domain. Cadherins mediate calcium-dependent cell-cell adhesion and play critical roles in normal tissue development. The classic cadherin subfamily includes N-, P-, R-, B-, and E-cadherins, as well as about ten other members that are found in adherens junctions, a cellular structure near the apical surface of polarized epithelial cells (2). 4F2hc is a transmembrane protein that belongs to the solute carrier family. 4F2hc forms heterodimeric complexes with various amino acid transporters, such as LAT1 and LAT2, and regulates uptake of amino acids (3). The 21-24 kDa integral proteins, caveolins, are the principal structural components of the cholesterol/sphingolipid-enriched plasma membrane microdomain caveolae. Three members of the caveolin family (caveolin-1, -2, and -3) have been identified with different tissue distributions (4). Ectonucleotide pyrophosphatase-phosphodiesterase 1 (ENPP1) is a single-pass, type II transmembrane protein primarily involved in ATP hydrolysis at the plasma membrane. ENPP1 plays important roles in bone mineralization and soft tissue calcification (5).

Background References

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

U.S. Patent No. 5,675,063.

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Limited Uses

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