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64097
Plasma Membrane Marker Antibody Sampler Kit
Primary Antibodies
Antibody Sampler Kit

Plasma Membrane Marker Antibody Sampler Kit #64097

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Plasma Membrane Marker Antibody Sampler Kit: Image 1
Western blot analysis of extracts from A172 and MCF7 cells using N-Cadherin (D4R1H) XP® Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).
Plasma Membrane Marker Antibody Sampler Kit: Image 2
Western blot analysis of extracts from A-431 cells, untreated (-) or treated with Human Epidermal Growth Factor (hEGF) #8916 (100 ng/ml, 2 min; +), and HeLa cells, untreated (-) or pervanadate-treated (1 mM, 15 min; +), using Phospho-Na,K-ATPase α1 (Tyr10) (E1Y9C) Rabbit mAb (upper) and Na,K-ATPase Antibody #3010 (lower).
Plasma Membrane Marker Antibody Sampler Kit: Image 3
Western blot analysis of extracts from SK-MEL-28, SNB19 and A-204 cells using Na,K-ATPase α1 (D4Y7E) Rabbit mAb (upper) or α-Actinin (D6F6) XP® Rabbit mAb #6487 (lower).
Plasma Membrane Marker Antibody Sampler Kit: Image 4
Western blot analysis of extracts from various cell lines, using E-Cadherin (24E10) Rabbit mAb.
Plasma Membrane Marker Antibody Sampler Kit: Image 5
Western blot analysis of extracts from various cell types using Caveolin-1 (D46G3) XP® Rabbit mAb.
Plasma Membrane Marker Antibody Sampler Kit: Image 6
Western blot analysis of extracts from various cell lines, using Pan-Cadherin (28E12) Rabbit mAb.
Plasma Membrane Marker Antibody Sampler Kit: Image 7
Western blot analysis of extracts from various cell lines using 4F2hc/CD98 (D3F9D) XP® Rabbit mAb (upper) and β-actin (D6A8) Rabbit mAb #8457 (lower).
Plasma Membrane Marker Antibody Sampler Kit: Image 8
Western blot analysis of extracts from Hep G2 and MDA-MB-231 cells using ENPP1 (D37B7) Rabbit mAb.
Plasma Membrane Marker Antibody Sampler Kit: Image 9
After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.
Plasma Membrane Marker Antibody Sampler Kit: Image 10
Immunohistochemical analysis of paraffin-embedded human non-Hodgkin’s Lymphoma using N-Cadherin (D4R1H) XP® Rabbit mAb performed on the Leica® BOND Rx.
Plasma Membrane Marker Antibody Sampler Kit: Image 11
Immunohistochemical analysis of paraffin-embedded SK-MEL-28 (left) and A-204 (right) cell pellets using Na,K-ATPase α1 (D4Y7E) Rabbit mAb.
Plasma Membrane Marker Antibody Sampler Kit: Image 12
Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using E-Cadherin (24E10) Rabbit mAb.
Plasma Membrane Marker Antibody Sampler Kit: Image 13
Western blot analysis of extracts from control HeLa cells (lane 1) or caveolin-1 knockout HeLa cells (lane 2) using Caveolin-1 (D46G3) XP® Rabbit mAb #3267 (upper), or β-actin (D6A8) Rabbit mAb #8457 (lower). The absence of signal in the caveolin-1 knockout HeLa cells confirms the specificity of the antibody to caveolin-1.
Plasma Membrane Marker Antibody Sampler Kit: Image 14
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using 4F2hc/CD98 (D3F9D) XP® Rabbit mAb.
Plasma Membrane Marker Antibody Sampler Kit: Image 15
Immunohistochemical analysis of paraffin-embedded human granulosa cell tumor of the ovary using N-Cadherin (D4R1H) XP® Rabbit mAb performed on the Leica® BOND Rx.
Plasma Membrane Marker Antibody Sampler Kit: Image 16
Immunohistochemical analysis of paraffin-embedded human appendix using Na,K-ATPase α1 (D4Y7E) Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).
Plasma Membrane Marker Antibody Sampler Kit: Image 17
Immunohistochemical analysis of paraffin-embedded human metastatic adenocarcinoma in lymph node, using E-Cadherin (24E10) Rabbit mAb.
Plasma Membrane Marker Antibody Sampler Kit: Image 18
Immunoprecipitation of caveolin-1 from HeLa cells using Caveolin-1 (D46G3) XP® Rabbit mAb followed by western blot using the same antibody. Lane 1 is 5% input.
Plasma Membrane Marker Antibody Sampler Kit: Image 19
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using 4F2hc/CD98 (D3F9D) XP® Rabbit mAb.
Plasma Membrane Marker Antibody Sampler Kit: Image 20
Immunohistochemical analysis of paraffin-embedded human gastric carcinoma using N-Cadherin (D4R1H) XP® Rabbit mAb performed on the Leica® BOND Rx.
Plasma Membrane Marker Antibody Sampler Kit: Image 21
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using Na,K-ATPase α1 (D4Y7E) Rabbit mAb.
Plasma Membrane Marker Antibody Sampler Kit: Image 22
Immunohistochemical analysis of paraffin-embedded mouse lung using E-Cadherin (24E10) Rabbit mAb.
Plasma Membrane Marker Antibody Sampler Kit: Image 23
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Caveolin-1 (D46G3) XP® Rabbit mAb.
Plasma Membrane Marker Antibody Sampler Kit: Image 24
Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma using 4F2hc/CD98 (D3F9D) XP® Rabbit mAb.
Plasma Membrane Marker Antibody Sampler Kit: Image 25
Immunohistochemical analysis of paraffin-embedded human colon using N-Cadherin (D4R1H) XP® Rabbit mAb. Note staining of myenteric plexus.
Plasma Membrane Marker Antibody Sampler Kit: Image 26
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using E-Cadherin (24E10) Rabbit mAb in the presence of control peptide (left) or E-Cadherin Blocking Peptide #1050 (right).
Plasma Membrane Marker Antibody Sampler Kit: Image 27
Immunohistochemical analysis of paraffin-embedded human lymphoma using Caveolin-1 (D46G3) XP® Rabbit mAb.
Plasma Membrane Marker Antibody Sampler Kit: Image 28
Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma using N-Cadherin (D4R1H) XP® Rabbit mAb.
Plasma Membrane Marker Antibody Sampler Kit: Image 29
Confocal immunofluorescent analysis of SK-MEL-28 (left), SNB19 (middle) or A-204 (right) cells using Na,K-ATPase α1 (D4Y7E) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Plasma Membrane Marker Antibody Sampler Kit: Image 30
Immunohistochemical analysis of paraffin-embedded mouse lung using Caveolin-1 (D46G3) XP® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).
Plasma Membrane Marker Antibody Sampler Kit: Image 31
Confocal immunofluorescent analysis of HeLa (left, high expressing) or SH-SY5Y (right, low expressing) cells using 4F2hc/CD98 (D3F9D) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Plasma Membrane Marker Antibody Sampler Kit: Image 32
Immunohistochemical analysis of paraffin-embedded A172 (positive, left) and MCF7 (negative, right) cell pellets using N-Cadherin (D4R1H) XP® Rabbit mAb.
Plasma Membrane Marker Antibody Sampler Kit: Image 33
Confocal immunofluorescent images of MCF7 cells using E-Cadherin (24E10) Rabbit mAb (green, left) compared to an isotype control (right). Blue pseudocolor = DRAQ5® (fluorescent DNA dye).
Plasma Membrane Marker Antibody Sampler Kit: Image 34
Confocal immunofluorescent analysis of C2C12 cells using Caveolin-1 (D46G3) XP® Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® (fluorescent DNA dye).
Plasma Membrane Marker Antibody Sampler Kit: Image 35
Flow cytometric analysis of SH-SY5Y cells (blue) and HeLa cells (green) using 4F2hc/CD98 (D3F9D) XP® Rabbit mAb (solid lines) or a concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed lines). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.
Plasma Membrane Marker Antibody Sampler Kit: Image 36
Confocal immunofluorescent analysis of A172 (positive, left) and MCF7 (negative, right) cells using N-Cadherin (D4R1H) XP® Rabbit mAb (green). Blue pseudocolor= DRAQ5® #4084 (fluorescent DNA dye).
Plasma Membrane Marker Antibody Sampler Kit: Image 37
Flow cytometric analysis of fixed and permeabilized HeLa cells (blue, negative) and MCF7 cells (green, positive) using E-Cadherin (24E10) Rabbit mAb.
Plasma Membrane Marker Antibody Sampler Kit: Image 38
Flow cytometric analysis of HeLa cells using Caveolin-1 (D46G3) XP® Rabbit mAb (solid line) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed line). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.
To Purchase # 64097T
Product # Size Price
64097T
1 Kit  (8 x 20 microliters) $ 570

Product Includes Quantity Applications Reactivity MW(kDa) Isotype
Phospho-Na,K-ATPase α1 (Tyr10) (E1Y9C) Rabbit mAb 13566 20 µl
  • WB
H M 100 Rabbit IgG
Pan-Cadherin (28E12) Rabbit mAb 4073 20 µl
  • WB
H M R 130-150 Rabbit IgG
E-Cadherin (24E10) Rabbit mAb 3195 20 µl
  • WB
  • IHC
  • IF
  • F
H M 135 Rabbit IgG
4F2hc/CD98 (D3F9D) XP® Rabbit mAb 47213 20 µl
  • WB
  • IHC
  • IF
  • F
H 75-120 Rabbit IgG
Caveolin-1 (D46G3) XP® Rabbit mAb 3267 20 µl
  • WB
  • IP
  • IHC
  • IF
  • F
H M R Hm Mk B Dg 21, 24 Rabbit IgG
N-Cadherin (D4R1H) XP® Rabbit mAb 13116 20 µl
  • WB
  • IP
  • IHC
  • IF
H M 140 Rabbit IgG
ENPP1 (D37B7) Rabbit mAb 5342 20 µl
  • WB
H 140 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
  • WB
Goat 
Na,K-ATPase α1 (D4Y7E) Rabbit mAb 23565 20 µl
  • WB
  • IHC
  • IF
H 100 Rabbit IgG

Product Description

The Plasma Membrane Marker Antibody Sampler Kit provides an economical means of detecting plasma membrane markers in various cell types. The kit includes enough antibodies to perform two western blot experiments with each primary antibody.

Specificity / Sensitivity

Each antibody in the Plasma Membrane Marker Antibody Sampler Kit detects endogenous levels of its target protein. Phospho-Na,K-ATPase α1 (Tyr10) (E1Y9C) Rabbit mAb recognizes endogenous levels of Na,K-ATPase α1 protein only when phosphorylated at Tyr10, and cross-reacts with an induced 75-80 kDa doublet of unknown origin. Pan-Cadherin (28E12) Rabbit mAb detects endogenous levels of total cadherin proteins. 4F2hc/CD98 (D3F9D) XP® Rabbit mAb recognizes endogenous levels of total 4F2hc/CD98 protein, and is predicted to detect multiple isoforms of 4F2hc/CD98. 

Source / Purification

Monoclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to residues surrounding Pro12 of human Na,K-ATPase α1, Pro780 of human E-cadherin, Arg526 of human N-cadherin, Val509 of human 4F2hc/CD98, Glu20 of human caveolin-1, Leu520 of human ENPP1, a conserved region of human N-, R-, E- and P-cadherin proteins, or a synthetic phosphopeptide corresponding to residues surrounding Tyr10 of human Na,K-ATPase α1 protein.

Background

The Na,K-ATPase is an integral membrane heterodimer belonging to the P-type ATPase family. Phosphorylation of Na,K-ATPase at Tyr10 has been implicated in the regulation of enzyme activity in response to hormones and neurotransmitters (1). Cadherins are a superfamily of transmembrane glycoproteins that contain cadherin repeats of approximately 100 residues in their extracellular domain. Cadherins mediate calcium-dependent cell-cell adhesion and play critical roles in normal tissue development. The classic cadherin subfamily includes N-, P-, R-, B-, and E-cadherins, as well as about ten other members that are found in adherens junctions, a cellular structure near the apical surface of polarized epithelial cells (2). 4F2hc is a transmembrane protein that belongs to the solute carrier family. 4F2hc forms heterodimeric complexes with various amino acid transporters, such as LAT1 and LAT2, and regulates uptake of amino acids (3). The 21-24 kDa integral proteins, caveolins, are the principal structural components of the cholesterol/sphingolipid-enriched plasma membrane microdomain caveolae. Three members of the caveolin family (caveolin-1, -2, and -3) have been identified with different tissue distributions (4). Ectonucleotide pyrophosphatase-phosphodiesterase 1 (ENPP1) is a single-pass, type II transmembrane protein primarily involved in ATP hydrolysis at the plasma membrane. ENPP1 plays important roles in bone mineralization and soft tissue calcification (5).
  1. Therien, A.G. and Blostein, R. (2000) Am J Physiol Cell Physiol 279, C541-66.
  2. Wheelock, M.J. and Johnson, K.R. (2003) Annu Rev Cell Dev Biol 19, 207-35.
  3. Kanai, Y. et al. (1998) J Biol Chem 273, 23629-32.
  4. Okamoto, T. et al. (1998) J Biol Chem 273, 5419-22.
  5. Harmey, D. et al. (2004) Am J Pathol 164, 1199-209.

Pathways & Proteins

Explore pathways + proteins related to this product.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
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U.S. Patent No. 5,675,063.