WB
H
Endogenous
78, 80
Rabbit IgG
#P07225
5627
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human PROS1 protein.
Background
PROS1 (PROS, Vitamin K-dependent protein S) is a vitamin K-dependent functional protein. It has an N-terminal Gla domain containing multiple Asp carboxylation sites, followed by four EGF repeats, and two C-terminal LG domains. Vitamin K mediates multiple carboxylations of glutamic acid residues in the PROS1 Gla domain. These modifications are required for PROS1 to exert its functions (1).
PROS1 is well known for its anticoagulant role during thrombosis (2,3). The anticoagulation function of PROS1 is exerted by both intrinsic and extrinsic mechanisms. Extrinsically, PROS1 functions as a cofactor in the TFPI (tissue factor pathway inhibitor) to inhibit Factor Xa generation and prevent coagulation (4). Intrinsically, PROS1 functions as a cofactor for activated protein C (aPC) (4). PROS1-aPC promotes degradation of Factors V and VIII, and prevents coagulation and thrombin formation (5). Another important function of PROS1 is as a ligand activator of the TAM (Tyro3, Axl, and MerTK) RTK family receptor. Among the TAM family members, PROS1 binds and activates Tyro3 and MerTK (6-8). The ligand/receptor interactions activate multiple downstream signaling pathways, such as STAT, ERK, Akt, NF-kB, Bcl-2, and FAK (9). PROS1/TAM interactions play important roles in immune and nervous inflammation and homeostasis, cancer development, and immune-related multiple sclerosis (9-11).
- Hafizi, S. and Dahlbäck, B. (2006) FEBS J 273, 5231-44.
- Rothlin, C.V. et al. (2015) Annu Rev Immunol 33, 355-91.
- Suleiman, L. et al. (2013) Crit Rev Oncol Hematol 88, 637-54.
- Gierula, M. and Ahnström, J. (2020) J Thromb Haemost 18, 2801-2811.
- Fernández, J.A. et al. (2009) Haematologica 94, 1721-31.
- Stitt, T.N. et al. (1995) Cell 80, 661-70.
- Tsou, W.I. et al. (2014) J Biol Chem 289, 25750-63.
- Lew, E.D. et al. (2014) Elife 3, e03385. doi: 10.7554/eLife.03385.
- Aehnlich, P. et al. (2021) Cancers (Basel) 13, 1195. doi: 10.3390/cancers13061195.
- Burstyn-Cohen, T. and Hochberg, A. (2021) Brain Plast 7, 33-46.
- Peeters, M.J.W. et al. (2020) Cancer Immunol Immunother 69, 237-244.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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