WB, IP, ChIP
H M R Mk
Endogenous
53
Rabbit IgG
#Q9NW64
55696
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
| Chromatin IP | 1:50 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln13 of human RBM22 protein.
Background
RBM22, known as Cwc2 in yeast, is an RNA-binding protein initially discovered to play a role in calcium signal by binding to ALG-2 (1). RBM22 helps form an active conformation of spliceosome catalytic RNA elements such as the U6 internal stem-loop and the U12-U6atac snRNA complexes (2,3). RBM22 helps control various methods of alternative splicing, as knockdown induces both exon skipping and intron retention (4). RBM22 has also been shown to bind DNA and is implicated in transcriptional regulation (5). Cell cycle and differentiation have also been shown to be affected by RBM22 deficiency, as loss of RBM22 causes cytokinesis defects and impaired erythroid differentiation in hematopoietic stem cells (6,7). RBM22 affects the growth and survival of glioblastoma and leukemia (8,9).
- Montaville, P. et al. (2006) Biochim Biophys Acta 1763, 1335-43.
- Rasche, N. et al. (2012) EMBO J 31, 1591-604.
- Ciavarella, J. et al. (2020) ACS Omega 5, 23549-23558.
- Van Nostrand, E.L. et al. (2020) Nature 583, 711-719.
- Xiao, R. et al. (2019) Cell 178, 107-121.e18.
- Kittler, R. et al. (2004) Nature 432, 1036-40.
- Ebert, B.L. et al. (2008) Nature 451, 335-9.
- Fuentes-Fayos, A.C. et al. (2020) Brain 143, 3273-3293.
- Yamauchi, T. et al. (2018) Cancer Cell 33, 386-400.e5.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation ChIP: Chromatin IP
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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