Render Target: STATIC
Render Timestamp: 2025-03-14T10:37:07.907Z
Commit: a619ae74f66dae0f27639e88da12bcf600e46428
XML generation date: 2025-03-07 13:19:04.081
Product last modified at: 2025-01-01T09:01:12.760Z
Cell Signaling Technology Logo
1% for the planet logo
PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

RBM22 (E9E6Z) Rabbit mAb #87340

Filter:
  • WB
  • IP
  • ChIP

    Supporting Data

    REACTIVITY H M R Mk
    SENSITIVITY Endogenous
    MW (kDa) 53
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    • ChIP-Chromatin Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    For optimal ChIP results, use 10 μL of antibody and 10 μg of chromatin (approximately 4 × 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.
    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50
    Chromatin IP 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    RBM22 (E9E6Z) Rabbit mAb recognizes endogenous levels of total RBM22 protein.

    Species Reactivity:

    Human, Mouse, Rat, Monkey

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln13 of human RBM22 protein.

    Background

    RBM22, known as Cwc2 in yeast, is an RNA-binding protein initially discovered to play a role in calcium signal by binding to ALG-2 (1). RBM22 helps form an active conformation of spliceosome catalytic RNA elements such as the U6 internal stem-loop and the U12-U6atac snRNA complexes (2,3). RBM22 helps control various methods of alternative splicing, as knockdown induces both exon skipping and intron retention (4). RBM22 has also been shown to bind DNA and is implicated in transcriptional regulation (5). Cell cycle and differentiation have also been shown to be affected by RBM22 deficiency, as loss of RBM22 causes cytokinesis defects and impaired erythroid differentiation in hematopoietic stem cells (6,7). RBM22 affects the growth and survival of glioblastoma and leukemia (8,9).
    For Research Use Only. Not For Use In Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    All other trademarks are the property of their respective owners. Visit our Trademark Information page.