WB, IP
H M R Mk
Endogenous
90-95
Rabbit IgG
#Q14BN4
7871
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Thr462 of human SLMAP protein.
Background
Sarcolemmal membrane-associated protein (SLMAP) is a tail-anchored membrane protein involved in the docking and membrane fusion process (1). It is also a component of a conserved striatin-interacting phosphatase and kinase complex (STRIPAK) which participates in a variety of cellular processes, including signaling, cell cycle control, cell migration, Golgi assembly, and apoptosis (2-4). There are several isoforms of SLMAP as a result of alternative splicing; shorter isoforms exhibit high expression in cardiac muscle, whereas the longer isoforms are expressed more ubiquitously, but at lower levels (5). As a cardiac membrane protein, SLMAP plays an important role in excitation-contraction coupling and the adrenergic response, and has been identified as a novel regulator of cardiac function (6-8). In support of that function, mutations in the SLMAP gene are associated with Brugada syndrome, a cardiac channelopathy (9). Deregulation of SLMAP expression has also been implicated in type 2 diabetes, and changes in SLMAP expression are linked to hyperglycemia and diabetes (10). Additionally, a novel SLMAP-ALK fusion has been identified in a patient with non-small cell lung cancer (11).
- Goudreault, M. et al. (2009) Mol Cell Proteomics 8, 157-71.
- Hwang, J. and Pallas, D.C. (2014) Int J Biochem Cell Biol 47, 118-48.
- Shi, Z. et al. (2016) Oncogene 35, 4549-57.
- Tang, Y. et al. (2019) Cell Discov 5, 3.
- Wigle, J.T. et al. (1997) J Biol Chem 272, 32384-94.
- Guzzo, R.M. et al. (2005) Am J Physiol Heart Circ Physiol 288, H1810-9.
- Nader, M. et al. (2012) Am J Physiol Heart Circ Physiol 302, H1138-45.
- Nader, M. (2019) Eur J Pharmacol 858, 172491.
- Ishikawa, T. et al. (2012) Circ Arrhythm Electrophysiol 5, 1098-107.
- Ding, H. et al. (2005) Am J Physiol Heart Circ Physiol 289, H206-11.
- Pagan, C. et al. (2019) Cold Spring Harb Mol Case Stud 5, a003939. doi: 10.1101/mcs.a003939.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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