WB
H M R
Endogenous
45-60
Rabbit
#Q9UBP0
6683
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln226 of human Spastin protein. Antibodies are purified by peptide affinity chromatography.
Background
Spastin (SPG4 or SPST) is a member of the ATPases associated with multiple cellular activities (AAA) protein family that contains a microtubule interacting and organelle transport (MIT) domain. There are four spastin isoforms, which are imported into the nucleus. One of the isoforms contains two nuclear export signals that efficiently drive export to the cytoplasm (1,2). Spastin is involved in severing microtubules that are polyglutaminated and also regulates microtubule abundance and mobility (3). Spastin promotes endosomal tubule fission by the interaction of the ER-localized spastin isoform M1-spastin and the endosomal IST1 protein at ER-endosome contacts. Rescue of endosomal tubulation phenotypes requires spastin's microtubule-severing ATPase capacity and its ability to bind the endosomal proteins IST1 and CHMP1B (4). Spastin regulates microtubule stability to modulate synaptic structure and function, and it is essential for the synaptic formation and axonal growth in developing neurons (1,5). Spastin is linked to several diseases including Hereditary Spastic Paraplegia (HSP) that affects upper motor neurons within the tubular ER membrane in corticospinal neurons (6). Some data suggest that spastin interacts with Lewy bodies and liquid droplets in Parkinson's disease (7). The missorting of Tau in Alzheimer's disease is strongly related to the formation of Tau oligomers and aggregates, and induces a mislocalization of TTLL6 (Tubulin-Tyrosine-Ligase-Like 6). TTLL6 induces the polyglutamylation of microtubules that triggers spastin-mediated severing of dendritic microtubules (8).
- Trotta, N. et al. (2004) Curr Biol 14, 1135-47.
- Claudiani, P. et al. (2005) Exp Cell Res 309, 358-69.
- Solowska, J.M. et al. (2014) J Neurosci 34, 1856-67.
- Allison, R. et al. (2017) J Cell Biol 216, 1337-55.
- Jardin, N. et al. (2018) Development 145, dev162701. doi: 10.1242/dev.162701.
- Park, S.H. et al. (2010) J Clin Invest 120, 1097-110.
- Papadopoulos, C. et al. (2015) PLoS Genet 11, e1005149.
- Zempel, H. and Mandelkow, E.M. (2015) Mol Neurodegener 10, 68.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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