Cat. # | Size | Qty. | Price |
---|---|---|---|
79310S | 100 µl |
|
REACTIVITY | H M R |
SENSITIVITY | Endogenous |
MW (kDa) | 45-60 |
SOURCE | Rabbit |
Product Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
NOTE: Please refer to primary antibody product webpage for recommended antibody dilution.
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalent grade water.
Load 20 µl onto SDS-PAGE gel (10 cm x 10 cm).
NOTE: Loading of prestained molecular weight markers (#59329, 10 µl/lane) to verify electrotransfer and biotinylated protein ladder (#7727, 10 µl/lane) to determine molecular weights are recommended.
NOTE: Volumes are for 10 cm x 10 cm (100 cm2) of membrane; for different sized membranes, adjust volumes accordingly.
* Avoid repeated exposure to skin.
posted June 2005
revised June 2020
Protocol Id: 263
Human, Mouse, Rat
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln226 of human Spastin protein. Antibodies are purified by peptide affinity chromatography.
Spastin (SPG4 or SPST) is a member of the ATPases associated with multiple cellular activities (AAA) protein family that contains a microtubule interacting and organelle transport (MIT) domain. There are four spastin isoforms, which are imported into the nucleus. One of the isoforms contains two nuclear export signals that efficiently drive export to the cytoplasm (1,2). Spastin is involved in severing microtubules that are polyglutaminated and also regulates microtubule abundance and mobility (3). Spastin promotes endosomal tubule fission by the interaction of the ER-localized spastin isoform M1-spastin and the endosomal IST1 protein at ER-endosome contacts. Rescue of endosomal tubulation phenotypes requires spastin's microtubule-severing ATPase capacity and its ability to bind the endosomal proteins IST1 and CHMP1B (4). Spastin regulates microtubule stability to modulate synaptic structure and function, and it is essential for the synaptic formation and axonal growth in developing neurons (1,5). Spastin is linked to several diseases including Hereditary Spastic Paraplegia (HSP) that affects upper motor neurons within the tubular ER membrane in corticospinal neurons (6). Some data suggest that spastin interacts with Lewy bodies and liquid droplets in Parkinson's disease (7). The missorting of Tau in Alzheimer's disease is strongly related to the formation of Tau oligomers and aggregates, and induces a mislocalization of TTLL6 (Tubulin-Tyrosine-Ligase-Like 6). TTLL6 induces the polyglutamylation of microtubules that triggers spastin-mediated severing of dendritic microtubules (8).
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