WB, IP
H
Endogenous
205
Rabbit
#Q5VYS8
79670
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val741 of human TUT7 protein. Antibodies are purified by peptide affinity chromatography.
Background
Terminal uridylyl transferase 4 and 7 (TUT4/7), also known as ZCCHC11 and ZCCHC6 respectively, are enzymes that posttranscriptionally uridylate mRNAs and miRNAs at the 3’ end (1,2). The TUT enzymes can monouridylate double-stranded RNA with a single nucleotide overhang, which in turn creates a recognition site for Dicer to process miRNAs (3). Many of the miRNAs recognized by TUT4/7 are involved in cell differentiation such as the HOX gene cluster (4). TUT4 and TUT7 have been shown to be recruited by Lin28 in stem cells to uridylate and block expression of let-7 miRNA, a microRNA with tumor suppressive qualities (3,5,6). TUT4 and TUT7 play a role in the mRNA decay pathway, where they polyuridylate targeted mRNAs to promote turnover (7). TUT4 and TUT7 have also been implicated in T cell activation, innate immune response, and antiviral defenses (8-10). Both TUT proteins have also been shown to prevent retrotransposition of LINE-1 elements, indicating a role in maintaining genomic stability (11).
- Rissland, O.S. et al. (2007) Mol Cell Biol 27, 3612-24.
- Wyman, S.K. et al. (2011) Genome Res 21, 1450-61.
- Heo, I. et al. (2012) Cell 151, 521-32.
- Thornton, J.E. et al. (2014) Nucleic Acids Res 42, 11777-91.
- Thornton, J.E. et al. (2012) RNA 18, 1875-85.
- Kim, B. et al. (2015) EMBO J 34, 1801-15.
- Lim, J. et al. (2014) Cell 159, 1365-76.
- Gutiérrez-Vázquez, C. et al. (2017) RNA 23, 882-891.
- Kozlowski, E. et al. (2017) PLoS One 12, e0179797.
- Le Pen, J. et al. (2018) Nat Struct Mol Biol 25, 778-786.
- Warkocki, Z. et al. (2018) Cell 174, 1537-1548.e29.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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