Render Target: STATIC
Render Timestamp:
3/25/2025, 7:30:05 AM EDT
3/25/2025, 11:30:05 AM UTC
Commit: 8d93f7ebe45006d66c127727d817fc3f57c4fe9a
XML generation date: 2025-03-07 13:15:55.643
Product last modified at: 2025-01-01T09:03:41.992Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

TUT7 Antibody #46970

Filter:
  • WB
  • IP

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 205
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:100

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    TUT7 Antibody recognizes endogenous levels of total TUT7 protein.

    Species Reactivity:

    Human

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val741 of human TUT7 protein. Antibodies are purified by peptide affinity chromatography.

    Background

    Terminal uridylyl transferase 4 and 7 (TUT4/7), also known as ZCCHC11 and ZCCHC6 respectively, are enzymes that posttranscriptionally uridylate mRNAs and miRNAs at the 3’ end (1,2). The TUT enzymes can monouridylate double-stranded RNA with a single nucleotide overhang, which in turn creates a recognition site for Dicer to process miRNAs (3). Many of the miRNAs recognized by TUT4/7 are involved in cell differentiation such as the HOX gene cluster (4). TUT4 and TUT7 have been shown to be recruited by Lin28 in stem cells to uridylate and block expression of let-7 miRNA, a microRNA with tumor suppressive qualities (3,5,6). TUT4 and TUT7 play a role in the mRNA decay pathway, where they polyuridylate targeted mRNAs to promote turnover (7). TUT4 and TUT7 have also been implicated in T cell activation, innate immune response, and antiviral defenses (8-10). Both TUT proteins have also been shown to prevent retrotransposition of LINE-1 elements, indicating a role in maintaining genomic stability (11).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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