This table compiles treatments that can be used as a positive control for our activation-state specific antibodies and should be seen as a starting point for trouble shooting. Please contact Technical Support for more detailed information or any further questions.

Note: To demonstrate phospho-specificity, extracts or nitrocellulose membranes should be subjected to phosphatase treatment. All UV‡ treatments were carried out using a Stratagene Stratalinker UV‡ Crosslinker. All products where “transfected” is indicated in the “cell line” column detect transfected levels of the respective target protein.

Find Antibody by Number:

Antibody Cell Line Treatment Control Extract
Antibody Cell Line Treatment Control Extract
Phospho-(Ser) 14-3-3 Binding Motif (4E2) Mouse mAb #9606JurkatCalyculin A (100 nM for 30 min)
Phospho-(Ser) 14-3-3 Binding Motif Antibody #9601JurkatCalyculin A (100 nM for 30 min)
Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb #2855MCF7 -AA/+Insulin (100 nM, 30 min)4E-BP1 Control Cell Extracts #53167
Phospho-4E-BP1 (Thr37/46) Antibody #9459MCF7 -AA/+Insulin (100 nM, 30 min)4E-BP1 Control Cell Extracts #53167
Phospho-4E-BP1 (Ser65) (174A9) Rabbit mAb #9456MCF7 -AA/+Insulin (100 nM, 30 min)4E-BP1 Control Cell Extracts #53167
Phospho-4E-BP1 (Ser65) (D9G1Q) Rabbit mAb #13443MCF7 -AA/+Insulin (100 nM, 30 min)4E-BP1 Control Cell Extracts #53167
Phospho-4E-BP1 (Ser65) Antibody #9451MCF7 -AA/+Insulin (100 nM, 30 min)4E-BP1 Control Cell Extracts #53167
Phospho-4E-BP1 (Thr70) (D7F6I) Rabbit mAb #13396MCF7 -AA/+Insulin (100 nM, 30 min)4E-BP1 Control Cell Extracts #53167
Phospho-4E-BP1 (Thr70) Antibody #9455MCF7 -AA/+Insulin (100 nM, 30 min)4E-BP1 Control Cell Extracts #53167
Phospho-53BP1 (Ser25/29) Antibody #2674HT-29UV‡ (100 mJ/cm2), 1 hr recovery before lysing
Phospho-53BP1 (Thr543) Antibody #3428HeLaUV‡ (100 mJ/cm2), 1 hr recovery before lysing
Phospho-53BP1 (Ser1618) (D4H11) Rabbit mAb #6209HeLaThymidine (2 mM for 16 hr) followed by Nocadazole (100 ng/ml for 24 hr)
Phospho-53BP1 (Ser1778) Antibody #2675HeLaUV‡ (100 mJ/cm2), 1 hr recovery before lysing
Phospho-c-Abl (Tyr89) (61A6) Rabbit mAb #3098K-562Untreated (K-562 cell line contains Bcr-Abl fusion)
Phospho-c-Abl (Tyr204) (C42B5) Rabbit mAb #3009K-562Untreated (K-562 cell line contains Bcr-Abl fusion)
Phospho-c-Abl (Tyr245) (73E5) Rabbit mAb #2868K-562Untreated (K-562 cell line contains Bcr-Abl fusion)
Phospho-c-Abl (Tyr245) Antibody #2861K-562Untreated (K-562 cell line contains Bcr-Abl fusion)
Phospho-c-Abl (Tyr412) (247C7) Rabbit mAb #2865K-562Untreated (K-562 cell line contains Bcr-Abl fusion)
Phospho-c-Abl (Thr735) Antibody #2864K-562Untreated (K-562 cell line contains Bcr-Abl fusion)
Phospho-Acetyl-CoA Carboxylase (Ser79) (D7D11) Rabbit mAb #11818C2C12Serum-starve overnight, AICAR (0.5 mM for 30 min)AMPK Control Cell Extracts #9158
Phospho-Acetyl-CoA Carboxylase (Ser79) Antibody #3661C2C12Serum-starve overnight, AICAR (0.5 mM for 30 min)AMPK Control Cell Extracts #9158
Phospho-Ack1 (Tyr284) Antibody #3138Transfected†Transfect cells with construct overexpressing Ack1
Phospho-Afadin (Ser1718) Antibody #5485A-431Serum-starve overnight, hEGF (100 ng/ml for 5 min)
Phospho-Akt (Thr308) (D25E6) XP® Rabbit mAb #13038JurkatCalyculin A (100 nM for 30 min)
Phospho-Akt (Thr308) (C31E5E) Rabbit mAb #2965JurkatCalyculin A (100 nM for 30 min)Akt Control Cell Extracts #9273
Phospho-Akt (Thr308) (244F9) Rabbit mAb #4056JurkatCalyculin A (100 nM for 30 min)Akt Control Cell Extracts #9273
Phospho-Akt (Thr308) (L32A4) Mouse mAb #5106JurkatCalyculin A (100 nM for 30 min)Akt Control Cell Extracts #9273
Phospho-Akt (Thr308) Antibody #9275JurkatCalyculin A (100 nM for 30 min)Akt Control Cell Extracts #9273
Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb #4060JurkatUntreated or Calyculin A (100 nM for 30 min); alternative: serum-starve, insulin (100 nM for 10 min); works for most cell linesAkt Control Cell Extracts #9273
Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb (APC Conjugate) #11962JurkatCalyculin A (100 nM for 30 min)
Phospho-Akt (Ser473) (193H12) Rabbit mAb #4058JurkatUntreated or Calyculin A (100 nM for 30 min); alternative: serum-starve, insulin (100 nM for 10 min); works for most cell linesAkt Control Cell Extracts #9273
Phospho-Akt (Ser473) (736E11) Rabbit mAb #3787LNCaPUntreatedSignalSlide® Phospho-Akt (Ser473) IHC Controls #8101
Phospho-Akt (Ser473) (587F11) Mouse mAb #4051JurkatUntreated or Calyculin A (100 nM for 30 min); alternative: serum-starve, insulin (100 nM for 10 min); works for most cell linesAkt Control Cell Extracts #9273
Phospho-Akt (Ser473) (D9W9U) Mouse mAb #12694JurkatCalyculin A (100 nM for 30 min)
Phospho-Akt (Ser473) Antibody #9271JurkatUntreated or Calyculin A (100 nM for 30 min); alternative: serum-starve, insulin (100 nM for 10 min); works for most cell linesAkt Control Cell Extracts #9273
Phospho-Akt Substrate (RXXS*/T*) (110B7E) Rabbit mAb #9614JurkatCalyculin A (100 nM for 30 min)
Phospho-Akt Substrate (RXXS*/T*) (110B7E) Rabbit mAb (HRP Conjugate) #6950JurkatCalyculin A (100 nM for 30 min)
Phospho-Akt (Thr450) (D5G4) Rabbit mAb #12178LNCaP
Phospho-(Ser/Thr) Akt Substrate Antibody #9611JurkatCalyculin A (100 nM for 30 min)
Phospho-Akt (Thr450) Antibody #9267JurkatCalyculin A (100 nM for 30 min)Akt Control Cell Extracts #9273
Phospho-Akt Substrate (RXRXXS*/T*) (23C8D2) Rabbit mAb #10001A431hEGF (100 ng/ml for 5 min)EGF Receptor Control Cell Extracts #5634
Phospho-Akt1 (Ser129) (D4P7F) Rabbit mAb #13461MCF7
Phospho-Akt1 (Ser473) (D7F10) XP® Rabbit mAb (Akt1 Specific) #9018JurkatCalyculin A (100 nM for 30 min)Akt Control Cell Extracts #9273
Phospho-Drosophila Akt (Ser505) Antibody #4054JurkatCalyculin A (100 nM for 30 min)Akt Control Cell Extracts #9273
Phospho-Akt2 (Ser474) (D3H2) Rabbit mAb (Akt2 Specific) #8599JurkatCalyculin A (100 nM for 30 min)Akt Control Cell Extracts #9273
Phospho-ALK (Tyr1078) (D28B4) Rabbit mAb #12127KARPAS-299Untreated (KARPAS-299 cell lines contains NPM-ALK fusion)
Phospho-ALK (Tyr1078) Antibody #4144KARPAS-299Untreated (KARPAS-299 cell lines contains NPM-ALK fusion)
Phospho-ALK (Tyr1096) (D96H9) Rabbit mAb #6962KARPAS-299Untreated (KARPAS-299 cell lines contains NPM-ALK fusion)
Phospho-ALK (Tyr1096) Antibody #4143KARPAS-299Untreated (KARPAS-299 cell lines contains NPM-ALK fusion)
Phospho-ALK (Tyr1278) (D59G10) Rabbit mAb #6941KARPAS-299Untreated (KARPAS-299 cell lines contains NPM-ALK fusion)
Phospho-ALK (Tyr1278) Antibody #3710KARPAS-299Untreated (KARPAS-299 cell lines contains NPM-ALK fusion)
Phospho-ALK (Tyr1278/1282/1283) Antibody #3983KARPAS-299Untreated (KARPAS-299 cell lines contains NPM-ALK fusion)
Phospho-ALK (Tyr1282/1283) (D39B2) Rabbit mAb #9687KARPAS-299Untreated (KARPAS-299 cell lines contains NPM-ALK fusion)
Phospho-ALK (Tyr1282/1283) Antibody #3714KARPAS-299Untreated (KARPAS-299 cell lines contains NPM-ALK fusion)
Phospho-ALK (Tyr1507) (D6F1V) Rabbit mAb #14678KARPAS-299Untreated (KARPAS-299 cell lines contains NPM-ALK fusion)
Phospho-ALK (Tyr1507) (D6F1V) Rabbit mAb #14678KARPAS-299Untreated
Phospho-ALK (Tyr1586) (3B4) Rabbit mAb #3348KARPAS-299Untreated (KARPAS-299 cell lines contains NPM-ALK fusion)
Phospho-ALK (Tyr1586) Antibody #3343KARPAS-299Untreated (KARPAS-299 cell lines contains NPM-ALK fusion)
Phospho-ALK (Tyr1604) Antibody #3341KARPAS-299Untreated (KARPAS-299 cell lines contains NPM-ALK fusion)
Cleaved α-Fodrin (Asp1185) Antibody #2121HeLaSerum-starve overnight, Staurosporine (1.0 μM for 4 hr)
Phospho-AML1 (Ser249) Antibody #4327Jurkat, HELUntreated
Phospho-(Ser/Thr) AMPK Substrate (P-S/T2-102) Rabbit mAb #5759C2C12Serum-starve 30 minAMPK Control Cell Extracts #9158
Phospho-AMPKα (Thr172) (40H9) Rabbit mAb #2535C2C12Serum-starve overnight, AICAR (0.5 mM for 30 min)AMPK Control Cell Extracts #9158
Phospho-AMPKα (Thr172) (D79.5E) Rabbit mAb #4188C2C12Serum-starve overnight, AICAR (0.5 mM for 30 min)AMPK Control Cell Extracts #9158
Phospho-AMPKα (Thr172) Antibody #2531C2C12Serum-starve overnight, AICAR (0.5 mM for 30 min)AMPK Control Cell Extracts #9158
Phospho-AMPKα1 (Ser485)/AMPKα2 (Ser491) Antibody #4185C2C12Serum-starve overnight, AICAR (0.5 mM for 30 min)AMPK Control Cell Extracts #9158
Phospho-AMPKα1 (Ser485) (45F5) Rabbit mAb #2537293Serum-starve overnight, AICAR (0.5 mM for 30 min)AMPK Control Cell Extracts #9158
Phospho-AMPKα1 (Ser485) Antibody #4184C6Serum-starve overnight, Oligomycin (0.5 μM for 30 min)AMPK Control Cell Extracts #9158
Phospho-AMPKβ1 (Ser108) Antibody #4181C2C12Serum-starve overnight, AICAR (0.5 mM for 30 min)AMPK Control Cell Extracts #9158
Phospho-AMPKβ1 (Ser182) Antibody #4186C2C12Serum-starve overnight, AICAR (0.5 mM for 30 min)AMPK Control Cell Extracts #9158
Phospho-AP2M1 (Thr156) (D4F3) Rabbit mAb #7399HeLaH2O2-treated (4 mM for 30 min)
Phospho-AP2M1 (Thr156) Antibody #3843HeLaH2O2 (4 mM for 30 min)
Phospho-APP (Thr668) (D90B8) Rabbit mAb #6986HeLaNocadazole (1 µg/ml for 18 hr)
Phospho-APP (Thr668) Antibody #3823HeLaNocadazole (1 µg/ml for 18 hr)
Phospho-ARHGAP42 (Tyr376) (D45E9) Rabbit mAb #5617HeLaUntreated
Phospho-β-Arrestin 1 (Ser412) (6-24) Mouse mAb #2416293Untreated
Phospho-AS160 (Ser318) (D3D11) Rabbit mAb #8619HeLaSerum-starve overnight, hIGF-1 (100 ng/ml for 15 min)
Phospho-AS160 (Ser588) (D8E4) Rabbit mAb #8730HeLaSerum-starve overnight, hIGF-1 (100 ng/ml for 15 min)
Phospho-AS160 (Thr642) (D27E6) Rabbit mAb #8881HeLaInsulin (150 nM for 15 min)
Phospho-AS160 (Thr642) Antibody #4288HeLaSerum-starve overnight, hIGF-I (100 ng/ml for 15 min)
Phospho-ASK1 (Ser83) Antibody #3761Transfected†Transfect with construct overexpressing ASK1
Phospho-ASK1 (Thr845) Antibody #3765Transfected†Transfect with construct overexpressing ASK1
Phospho-ASK1 (Ser967) Antibody #3764Transfected†Transfect with construct overexpressing ASK1
Phospho-ATF-2 (Thr69/71) Antibody #9225NIH/3T3Anisomycin (25 μg/ml for 30 min)ATF-2 Control Cell Extracts #9223
Phospho-ATF-2 (Thr71) (11G2) Rabbit mAb #5112NIH/3T3Anisomycin (25 μg/ml for 30 min)ATF-2 Control Cell Extracts #9223
Phospho-ATF-2 (Thr71) Antibody #9221NIH/3T3Anisomycin (25 μg/ml for 30 min)ATF-2 Control Cell Extracts #9223
Phospho-Atg13 (Ser355) Antibody #43533Transfected†Transfect with constructs overexpressing Atg13 and ULK1
Phospho-ATM (Ser1981) (D6H9) Rabbit mAb #5883293UV‡ (100 mJ/cm2), 1 hr recovery before lysingSAPK/JNK Control Cell Extracts #9253
Phospho-ATM (Ser1981) (D25E5) Rabbit mAb #13050HCT 116Neocarzinostatin (10 μM for 1 hr)SAPK/JNK Control Cell Extracts #9253
Phospho-ATM (Ser1981) (10H11.E12) Mouse mAb #4526293UV‡ (100 mJ/cm2), 1 hr recovery before lysingSAPK/JNK Control Cell Extracts #9253
Phospho-(Ser/Thr) ATM/ATR Substrate (4F7) Rabbit mAb #2909293UV‡ (50 mJ/cm2), 30 min recoverySAPK/JNK Control Cell Extracts #9253
Phospho-(Ser/Thr) ATM/ATR Substrate (S*/T*QG) (P-S/T2-100) Rabbit mAb #6966293UV‡ (50 mJ/cm2), 30 min recovery
Phospho-ATP-Citrate Lyase (Ser455) Antibody #4331NIH/3T3Serum-starve overnight, PDGF (50 ng/ml for 10 min)
Phospho-Na,K-ATPase α1 (Tyr10) (E1Y9C) Rabbit mAb #13566A-431, HeLa
Phospho-Na,K-ATPase α1 (Tyr10) Antibody #3060A-431Serum-starve overnight, hEGF (100 ng/ml for 5 min)
Phospho-Na,K-ATPase α1 (Ser16) Antibody #4020PC-12Serum-starve overnight, TPA (200 nM for 30 min)
Phospho-Na,K-ATPase α1 (Ser23) Antibody #4006PC-12Serum-starve overnight, TPA (200 nM for 30 min)
Phospho-ATR (Ser428) Antibody #2853HeLaUV‡ (100 mJ/cm2), 1 hr recovery before lysing
Phospho-ATRIP (Ser224) Antibody #5161HeLa, 293TUntreated
Phospho-Aurora A (Thr288) (C39D8) Rabbit mAb #3079HT-29Nocadazole (100 ng/ml for 18 hr) - treatment induces signal
Phospho-Aurora A (Thr288)/Aurora B (Thr232)/Aurora C (Thr198) (D13A11) XP® Rabbit mAb #2914HT-29Nocadazole (100 ng/ml for 18 hr) - treatment induces signal
Phospho-Axl (Tyr702) (D12B2) Rabbit mAb #5724NCI-H1299Gas6 (400 ng/ml for 5 min)
Phospho-Bad (Ser112) (40A9) Rabbit mAb #5284COSSerum-starve overnight, TPA (200 nM for 30 min)Bad Control Proteins #9293
Phospho-Bad (Ser112) (7E11) Mouse mAb #9296COSSerum-starve overnight, TPA (200 nM for 30 min)Bad Control Proteins #9293
Phospho-Bad (Ser112) Antibody #9291COSSerum-starve overnight, TPA (200 nM for 30 min)Bad Control Proteins #9293
Phospho-Bad (Ser136) (D25H8) Rabbit mAb #4366ACHNSerum-starve overnight, hEGF (100 ng/ml for 30 min)Bad Control Proteins #9293
Phospho-Bad (Ser136) (185D10) Rabbit mAb #5286COSSerum-starve overnight, TPA (200 nM for 30 min)Bad Control Proteins #9293
Phospho-Bad (Ser136) Antibody #9295Transfected†Serum-starve overnight, PDGF (100 ng/ml for 30 min)Bad Control Proteins #9293
Phospho-Bad (Ser155) Antibody #9297Transfected†Serum-starve overnight, PDGF (100 ng/ml for 30 min)Bad Control Proteins #9293
Phospho-BAP1 (Ser592) Antibody #9373HeLaSerum-starve overnight, UV‡ (50 mJ/cm2), 1 hr recovery
Phospho-Atg14 (Ser29) Antibody #13155C2C12Starved with Earle’s Balanced Salt Solution (EBSS) for 2 hr
Phospho-Bcl-2 (Thr56) Antibody (Human Specific) #2875RL-7Paclitaxel (50 nM for 20 hr)
Phospho-Bcl-2 (Ser70) (5H2) Rabbit mAb #2827JurkatPaclitaxel (1 μM for 20 hr)
Phospho-BCL9L (Ser915) Antibody #13325MCF7TPA (200 nM, 30 min)
Phospho-Bcr (Tyr177) Antibody #3901K-562Untreated (K-562 cell line contains Bcr-Abl fusion)
Phospho-Beclin-1 (Ser15) (D4B7R) Rabbit mAb #84966Transfected†Transfect with constructs overexpressing Beclin-1 and ULK1
Phospho-Beclin-1 (Ser93) (D9A5G) Rabbit mAb #14717A172CCCP (100 µM for 2 hr)
Phospho-Beclin-1 (Ser93/96) Antibody #12476Transfected†Transfect with construct overexpressing Beclin-1; CCCP (100 µM for 2 hr) - treatment induces signal
Phospho-Bim (Ser55) Antibody #4550A20Serum-starve overnight, TPA (200 nM for 30 min)
Phospho-Bim (Ser69) (D7E11) Rabbit mAb #4585Raji, A20Serum-starve overnight, TPA (200 nM for 30 min)
Phospho-Bim (Ser69) Antibody #4581Raji, A20Serum-starve overnight, TPA (200 nM for 30 min)
Phospho-Bim (Ser77) (D4H12) Rabbit mAb #12433Raji, A20Serum-starve overnight, TPA (200 nM for 30 min)
Phospho-BLNK (Tyr96) Antibody #3601RamosAnti-IgM (12 µg/ml for 2 min)
Phospho-BMAL1 (Ser42) Antibody #13936mouse brain, rat brainUntreated
Phospho-BRCA1 (Ser1524) Antibody #9009HeLaUV‡ (100 mJ/cm2), 1 hr recovery before lysing
Phospho-BACH1/BRIP1 (Thr1133) Antibody #11983293TUntreated
Phospho-Btk (Ser180) (3D3) Mouse mAb #3537THP-1Serum-starve overnight, H2O2 (2 mM for 2 min)
Phospho-Btk (Tyr223) Antibody #5082RamosAnti-IgM (12 μg/ml for 10 min)
Phospho-C/EBPα (Ser21) Antibody #2841differentiated 3T3-L1Serum-starve overnight, insulin (100 nM for 45 min)
Phospho-C/EBPα (Thr222/226) Antibody #2844U-937Untreated
Phospho-C/EBPbeta (Ser105) Antibody (Rat Specific) #3081PC-12Untreated
Phospho-C/EBPbeta (Thr235) Antibody #3084differentiated 3T3-L1Serum-starve overnight, insulin (100 nM for 10 min)
Phospho-CAD (Ser1859) Antibody #12662HeLaUntreated
Phospho-CaMKII (Tyr231) Antibody #3356Rat brain15 min ischemia followed by 4 hr reperfusion
Phospho-CaMKII (Thr286) (D21E4) Rabbit mAb #12716mouse brain, rat brainUntreated
Phospho-CaMKK2 (Ser511) Antibody #12818mouse brain, rat brainUntreated
Phospho-CARD11 (Ser652) Antibody #5189JurkatTPA (40 nM) and calcium ionophore A23187 (2 μM for 15 min)
Cleaved Caspase Substrate Motif [DE(T/S/A)D] MultiMab™ Rabbit mAb mix #8698HeLaStaurosporine #9953 (1 µM for 3 hr)
Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb #9664HeLaSerum-starve overnight, Staurosporine (1 μM for 3 hr)Caspase-3 Control Cell Extracts #9663
Cleaved Caspase-3 (Asp175) (D3E9) Rabbit mAb (PE Conjugate) #12768JurkatEtoposide (25 µM for 5 hr)
Cleaved Caspase-3 (Asp175) (D3E9) Rabbit mAb #9579Jurkat Etoposide (25 µM for 5 hr)
Cleaved Caspase-3 (Asp175) Antibody #9661HeLaSerum-starve overnight, Staurosporine (1 μM for 3 hr)Caspase-3 Control Cell Extracts #9663
Cleaved Caspase-1 (Asp297) (D57A2) Rabbit mAb #4199THP-1TPA (80 nM overnight) plus LPS (1 µg/ml for 8 hr)
Cleaved Caspase-6 (Asp162) Antibody #9761HeLaSerum-starve overnight, Staurosporine (1 μM for 3 hr)Caspase-3 Control Cell Extracts #9663
Cleaved Caspase-7 (Asp198) (D6H1) Rabbit mAb #8438JurketEtoposide (25 µM for 5 hr)Jurkat Apoptosis Cell Lysates (etoposide) #2043
Cleaved Caspase-7 (Asp198) Antibody #9491HeLaSerum-starve overnight, Staurosporine (1 μM for 3 hr)Caspase-3 Control Cell Extracts #9663
Cleaved Caspase-8 (Asp391) (18C8) Rabbit mAb #9496THP-1CHX (10 μg/ml) and hTNF-α (20 ng/ml) overnightJurkat Apoptosis Cell Lysates (etoposide) #2043
Cleaved Caspase-8 (Asp384) (11G10) Mouse mAb #9748THP-1CHX (10 μg/ml) and hTNF-α (20 ng/ml) overnightJurkat Apoptosis Cell Lysates (etoposide) #2043
Cleaved Caspase-8 (Asp387) (D5B2) XP® Rabbit mAb (Mouse Specific) #8592CTLL-2Cycloheximide (10 µg/ml overnight) plus mTNF-α (20 ng/ml for 4 hr)
Cleaved Caspase-8 (Asp387) Antibody (Mouse Specific) #9429CTLL-2Cycloheximide (10 µg/ml overnight) plus mTNF-α (20 ng/ml for 4 hr)
Cleaved Caspase-9 (Asp315) Antibody (Human Specific) #9505JurkatEtoposide (25 μM for 5 hr)Jurkat Apoptosis Cell Lysates (etoposide) #2043
Cleaved Caspase-9 (Asp353) Antibody (Mouse Specific) #9509L-929Serum-starve overnight, Staurosporine (1 µM for 3 hr)
Cleaved Caspase-9 (Asp353) Antibody (Rat Specific) #9507C6Serum-starve overnight, Staurosporine (1 µM for 3 hr)
Cleaved Caspase-9 (Asp330) (D2D4) Rabbit mAb #7237HeLaSerum-starve overnight, Staurosporine (1 µM for 3 hr)
Cleaved Caspase-9 (Asp330) Antibody (Human Specific) #9501JurkatEtoposide (25 μM for 5 hr)Jurkat Apoptosis Cell Lysates (etoposide) #2043
Phospho-β-Catenin (Ser33/37) Antibody #2009SW480Untreated
Phospho-β-Catenin (Ser33/37/Thr41) Antibody #9561SW480Untreated
Phospho-β-Catenin (Ser45) Antibody #9564SW480Untreated
Phospho-β-Catenin (Thr41/Ser45) Antibody #9565SW480Untreated
Phospho-β-Catenin (Ser552) (D8E11) Rabbit mAb #5651SK-N-MCForskolin (50 µM for 30 min)
Phospho-β-Catenin (Ser552) Antibody #9566SK-N-MCForskolin (50 μM for 30 min)
Phospho-β-Catenin (Ser675) (D2F1) XP® Rabbit mAb #4176SK-N-MCForskolin (50 μM for 30 min)
Phospho-β-Catenin (Ser675) Antibody #9567SK-N-MCForskolin (50 μM for 30 min)
Acetyl-β-Catenin (Lys49) (D7C2) Rabbit mAb #9030SW480
Acetyl-β-Catenin (Lys49) Antibody #9534Transfected†Transfect with a construct overexpressing CBP
Phospho-Catenin δ-1 (Tyr228) Antibody #2911A-431Serum-starve overnight, hEGF (100 ng/ml for 15 min)
Phospho-Catenin δ-1 (Ser252) Antibody #8477A-431Untreated
Phospho-Catenin δ-1 (Ser320) Antibody #8016A-431Untreated
Phospho-Catenin δ-1 (Tyr904) Antibody #2910A-431Serum-starve overnight, hEGF (100 ng/ml for 15 min)
Phospho-Caveolin-1 (Tyr14) Antibody #3251HeLaSerum-starve overnight, H2O2 (500 μM for 15 min)
Phospho-c-Cbl (Tyr700) (D16D7) Rabbit mAb #8869JurkatPervanadate (1 mM for 30 min)
Phospho-c-Cbl (Tyr731) Antibody #3554K-562Untreated
Phospho-c-Cbl (Tyr774) Antibody #3555K-562Untreated
Acetyl-CBP (Lys1535)/p300 (Lys1499) Antibody #4771NIH/3T3, 293TSA (400 nM overnight)
Phospho-Cyclin B1 (Ser116) Antibody #13495HeLaNocadazole (50 ng/ml for 20 hr)
Phospho-CD19 (Tyr531) Antibody #3571RamosAnti-IgM (12 μg/ml for 2 min)
Phospho-CD79A (Tyr182) Antibody #5173RamosAnti-IgM (12 μg/ml for 2 min)
Phospho-cdc2 (Thr14) Antibody #2543HeLaNocadazole (50 ng/ml for 20 hr)
Phospho-cdc2 (Tyr15) (10A11) Rabbit mAb #4539HeLaHydroxyurea (10 mM for 20 hr)
Phospho-cdc2 (Tyr15) Antibody #9111HeLaHydroxyurea (10 mM for 20 hr)
Phospho-cdc2 (Thr161) Antibody #9114HeLaNocadazole (50 ng/ml for 20 hr)
Phospho-CDC20 (Ser51) Antibody #8038293TUntreated
Phospho-cdc25C (Thr48) (D2H3) Rabbit mAb #12028HT-29Thymidine (2 mM for 16 hr), add fresh media and Nocadazole (100 ng/ml for 24 hr), collect by mitotic shake off
Phospho-cdc25C (Thr48) Antibody #9527HT-29Thymidine (2 mM for 16 hr), add fresh media and Nocadazole (100 ng/ml for 24 hr), collect by mitotic shake off
Phospho-cdc25C (Ser198) Antibody #9529Transfected†Transfect with construct overexpressing cdc25C; Nocadazole (100 ng/ml for 18 hr) - treatment induces signal
Phospho-cdc25C (Ser216) (63F9) Rabbit mAb #4901HT-29Nocadazole (100 ng/ml for 18 hr) - treatment induces signal
Phospho-cdc25C (Ser216) Antibody #9528HT-29Nocadazole (100 ng/ml for 18 hr) - treatment diminishes signal
Phospho-CDC37 (Ser13) (D8P8F) Rabbit mAb #13248293, C2C12, NIH/3T3, C2C12
Phospho-CDCP1 (Tyr707) Antibody #13111ZR-75
Phospho-CDCP1 (Tyr734) Antibody #9050HCT 116Cub1 mAb
Phospho-CDCP1 (Tyr743) (D2G2J) Rabbit mAb #14965DLD-1
Phospho-CDCP1 (Tyr806) Antibody #13024ZR-75
Phospho-CDK2 (Thr160) Antibody #2561HeLaHydroxyurea (10 mM for 18 hr)
Phospho-CDK9 (Thr186) Antibody #2549HeLaUntreated; site is constitutively phosphorylated
Phospho-CENP-A (Ser7) Antibody #2187HT-29, HeLaThymidine (2 mM for 12 hr), add fresh media and Paclitaxol (500 nM for 16 hr) - treatment induces signal
Phospho-Chk1 (Ser280) Antibody #2347HT-29UV‡ (100 mJ/cm2), 1 hr recovery before lysing
Phospho-Chk1 (Ser296) (D3O9F) Rabbit mAb #90178HeLaUV‡ (100 mJ/cm2), 2 hr recovery before lysing
Phospho-Chk1 (Ser296) Antibody #2349293UV‡ (100 mJ/cm2), 1 hr recovery before lysing
Phospho-Chk1 (Ser317) (D12H3) XP® Rabbit mAb #12302293UV‡ (100 mJ/cm2), 1 hr recovery before lysing
Phospho-Chk1 (Ser317) (D7H2) Rabbit mAb #8191293UV‡ (100 mJ/cm2), 1 hr recovery before lysing
Phospho-Chk1 (Ser317) Antibody #2344HeLaUV‡ (100 mJ/cm2), 1 hr recovery before lysing
Phospho-Chk1 (Ser345) (133D3) Rabbit mAb #2348HeLaUV‡ (100 mJ/cm2), 1 hr recovery before lysing
Phospho-Chk1 (Ser345) Antibody #2341HeLaUV‡ (100 mJ/cm2), 1 hr recovery before lysing
Phospho-Chk2 (Ser19) Antibody #2666HeLaUV‡ (100 mJ/cm2), 1 hr recovery before lysing
Phospho-Chk2 (Ser33/35) Antibody #2665HeLaUV‡ (100 mJ/cm2), 1 hr recovery before lysing
Phospho-Chk2 (Thr68) (C13C1) Rabbit mAb #2197HeLaUV‡ (100 mJ/cm2), 1 hr recovery before lysing
Phospho-Chk2 (Thr68) (C13C1) Rabbit mAb (PE Conjugate) #12812HeLaUV‡ (100 mJ/cm2), 2 hr recovery before lysing
Phospho-Chk2 (Thr68) Antibody #2661HeLaUV‡ (100 mJ/cm2), 1 hr recovery before lysing
Phospho-Chk2 (Ser516) Antibody #2669HeLaUV‡ (100 mJ/cm2), 1 hr recovery before lysing
Phospho-CK2 Substrate [(pS/pT)DXE] MultiMab™ Rabbit mAb mix #8738HeLa Calyculin A #9902 (10 nM, 30 min)
Phospho-Cofilin (Ser3) (77G2) Rabbit mAb #3313C2C12, NIH/3T3, HeLaUntreated
Phospho-Cofilin (Ser3) Antibody #3311C2C12, NIH/3T3, HeLaUntreated
Phospho-Cortactin (Tyr421) Antibody #4569HeLaSerum-starve overnight, H2O2 (2 nM for 2 min)
Phospho-CREB (Ser133) (87G3) Rabbit mAb #9198SK-N-MCForskolin (30 µM) and IBMX (0.5 mM), for 30 minCREB Control Cell Extracts #9193
Phospho-CREB (Ser133) (1B6) Mouse mAb #9196SK-N-MCForskolin (30 µM) and IBMX (0.5 mM), for 30 minCREB Control Cell Extracts #9193
Phospho-CrkII (Tyr221) Antibody #3491K-562Untreated
Phospho-CrkL (Tyr207) Antibody #3181K-562Untreated
Phospho-CRMP-2 (Thr514) Antibody #9397Rat brainUntreated
Phospho-M-CSF Receptor (Tyr546) Antibody #3083Transfected†Transfect cells with a construct overexpressing CSFR1
Phospho-M-CSF Receptor (Tyr699) (D10B11) Rabbit mAb #12251GDM1Human Macrophage Colony Stimulating Factor (hM-CSF) #8929 (100 ng/ml for 10 min; +)
Phospho-M-CSF Receptor (Tyr699) Antibody #3399NKM-1Untreated
Phospho-M-CSF Receptor (Tyr708) (D5F4Y) Rabbit mAb #14591GDM-1Serum-starve overnight; hM-CSF 100 ng/ml for 5 min
Phospho-M-CSF Receptor (Tyr708) (D5F4Y) Rabbit mAb #14591GDM-1Human Macrophage Colony Stimulating Factor (hM-CSF) #8929 (100 ng/ml for 5 min)
Phospho-M-CSF Receptor (Tyr708) Antibody #3080Transfected†Transfect cells with a construct overexpressing CSFR1
Phospho-M-CSF Receptor (Tyr723) (49C10) Rabbit mAb #3155NKM-1Untreated
Phospho-M-CSF Receptor (Tyr723) Antibody #3151NKM-1Untreated
Phospho-M-CSF Receptor (Tyr809) Antibody #3154NKM-1Untreated
Phospho-M-CSF Receptor (Tyr923) Antibody #3406NKM-1Untreated
Phospho-CTDSPL2 (Ser104) Antibody #5294HelaUntreated
Phospho-α-E-Catenin (Ser652) Antibody #13061MCF-7, NIH-3T3, COS
Phospho-α-E-Catenin (Ser655/Thr658) Antibody #13231HeLa, PANC-1, NIH-3T3
Phospho-Connexin 43 (Ser368) Antibody #3511COSSerum-starve overnight, TPA (200 nM for 30 min)
Phospho-Cyclin B1 (Ser133) (9E3) Rabbit mAb #4133HeLaNocadazole (50 ng/ml for 20 hr)
Phospho-Cyclin B1 (Ser147) Antibody #4131HeLaNocadazole (50 ng/ml for 20 hr)
Phospho-Cyclin D1 (Thr286) (D29B3) XP® Rabbit mAb #3300HT-1080MG-132 (10 µM for 4 hr)
Phospho-Cyclin E1 (Thr62) Antibody #4136MCF7Hydroxyurea (10 mM overnight)
Phospho-CYLD (Ser418) Antibody #4500293hTNF-α (20 ng/ml for 20 min)
Phospho-Dab1 (Tyr220) Antibody #3327Transfected†Cotransfect with Dab1 and fyn expression contructs
Phospho-Dab1 (Tyr232) Antibody #3325Transfected†Cotransfect with Dab1 and fyn expression contructs
Phospho-DAPP1/BAM32 (Tyr139) (D7G4G) Rabbit mAb #13703RamosAnti-IgM (12ug/mL for 10 min)
Phospho-DARPP-32 (Thr34) (D27A4) Rabbit mAb #12438MKN-45Forskolin (30 µM for 20 min)
Phospho-DARPP-32 (Thr75) Antibody #2301Rat brain, mouse brain, SHSY5Y, Neuro2APKA (12,500 U/100 μl for 1 hr)
Phospho-DARPP-32 (Ser97) (D11A5) Rabbit mAb #3401Mouse brain
Phospho-DBC1 (Thr454) Antibody #4880293UV‡ (100 mJ/cm2), 4 hr recovery
Cleaved Drosophila Dcp-1 (Asp216) Antibody #9578S2Cycloheximide (10 µM for 5 hr) or Actinomycin D (1 µM for 5 hr)
Phospho-DDR1 (Tyr792) Antibody #11994T47DCollagen (15 μg/ml)
Phospho-DDR1 (Tyr513) (E1N8F) Rabbit mAb #14531T47-DCollagen (20 μg/ml for 18 hr)
Phospho-DDR1 (Tyr513) (E1N8F) Rabbit mAb #14531T-47DCollagen (20 μg/ml for 18 hr)
Phospho-Desmoplakin (Ser165/166) Antibody #5885A-431Untreated
Cleaved DFF45 (Asp224) Antibody #9731JurkatEtoposide (25 µM for 5 hr)Jurkat Apoptosis Cell Lysates (etoposide) #2043
Di-Methyl Lysine Motif [dme-K] Rabbit mAb #14117HeLaAdenosine-2', 3'-dialdehyde (AdOx, 100 µM for 24 hr)
Phospho-DNAJC2/MPP11 (Ser47) Antibody #12397293
Phospho-p56Dok-2 (Tyr351) Antibody #3911Transfected†Transfect cells with a construct overexpressing p56Dok-2
Phospho-Doublecortin (Ser334) (D11B10) Rabbit mAb #14053Fetal rat brainUntreated
Phospho-Doublecortin (Ser297) Antibody #4605Fetal rat brainUntreated
Phospho-Doublecortin (Ser334) Antibody #3453Rat brain, mouse brainUntreated
Phospho-DR6 (Ser562) Antibody #7616A-431Etoposide (25 µM for 5 hr)
Phospho-DRP1 (Ser616) (D9A1) Rabbit mAb #4494HeLaNocadazole (100 mg/ml for 17 hr)
Phospho-DRP1 (Ser616) Antibody #3455HeLaNocadazole (100 mg/ml for 17 hr)
Phospho-DRP1 (Ser637) (D3A4) Rabbit mAb #6319PC-12Forskolin (20 μM for 1 hr)
Phospho-DRP1 (Ser637) Antibody #4867PC-12PKA (12,500 U/100 μl for 1 hr)
Phospho-DUSP1/MKP1 (Ser359) (125E2) Rabbit mAb #2857HeLaALLN (50 µM for 5–6 hr)
Phospho-eEF2 (Thr56) Antibody #2331C6Forskolin (10 µM for 1 hr)
Phospho-eEF2k (Ser366) Antibody #3691MCF7Serum-starve overnight, +hIGF-1 (100 ng/ml for 10 min)p70 S6 Kinase MCF7 Control Cell Extracts #34499
Phospho-EGF Receptor (Thr678) Rabbit Ab #14343A549TPA #4174 (200 nM, 15 min)
Phospho-EGF Receptor (Thr669) (D2F1) Rabbit mAb #8808A431Serum-starve overnight, hEGF (100 ng/ml for 5 min)EGF Receptor Control Cell Extracts #5634
Phospho-EGF Receptor (Thr669) Antibody #3056A431Serum-starve overnight, hEGF (100 ng/ml for 5 min)EGF Receptor Control Cell Extracts #5634
Phospho-EGF Receptor (Tyr845) (D63B4) Rabbit mAb #6963A431Serum-starve overnight, hEGF (100 ng/ml for 5 min)EGF Receptor Control Cell Extracts #5634
Phospho-EGF Receptor (Tyr845) Antibody #2231A431Serum-starve overnight, hEGF (100 ng/ml for 5 min)EGF Receptor Control Cell Extracts #5634
Phospho-EGF Receptor (Tyr998) (C24A5) Rabbit mAb #2641A431Serum-starve overnight, hEGF (100 ng/ml for 5 min)EGF Receptor Control Cell Extracts #5634
Phospho-EGF Receptor (Tyr992) Antibody #2235A431Serum-starve overnight, hEGF (100 ng/ml for 5 min)EGF Receptor Control Cell Extracts #5634
Phospho-EGF Receptor (Tyr1045) Antibody #2237A431Serum-starve overnight, hEGF (100 ng/ml for 5 min)EGF Receptor Control Cell Extracts #5634
Phospho-EGF Receptor (Ser1046/1047) Antibody #2238A431Serum-starve overnight, hEGF (100 ng/ml for 5 min)EGF Receptor Control Cell Extracts #5634
Phospho-EGF Receptor (Tyr1068) (D7A5) XP® Rabbit mAb #3777A431Serum-starve overnight, hEGF (100 ng/ml for 5 min)EGF Receptor Control Cell Extracts #5634
Phospho-EGF Receptor (Tyr1068) (D7A5) XP® Rabbit mAb (PE Conjugate) #14565N/A
Phospho-EGF Receptor (Tyr1068) (1H12) Mouse mAb #2236A431Serum-starve overnight, hEGF (100 ng/ml for 5 min)EGF Receptor Control Cell Extracts #5634
Phospho-EGF Receptor (Tyr1068) Antibody #2234A431Serum-starve overnight, hEGF (100 ng/ml for 5 min)EGF Receptor Control Cell Extracts #5634
Phospho-EGF Receptor (Tyr1086) Antibody #2220A431Serum-starve overnight, hEGF (100 ng/ml for 5 min)EGF Receptor Control Cell Extracts #5634
Phospho-EGF Receptor (Tyr1148) Antibody #4404A431Serum-starve overnight, hEGF (100 ng/ml for 5 min)EGF Receptor Control Cell Extracts #5634
Phospho-EGF Receptor (Tyr1173) (53A5) Rabbit mAb #4407A431Serum-starve overnight, hEGF (100 ng/ml for 5 min)EGF Receptor Control Cell Extracts #5634
Phospho-HER2/ErbB2 (Tyr1248)/EGFR (Tyr1173) Antibody #2244MCF7Serum-starve overnight, neuregulin (100 ng/ml for 5 min)
Phospho-eIF2α (Ser51) (D9G8) XP® Rabbit mAb #3398HeLaThapsigargin (300 nM for 30 min)
Phospho-eIF2α (Ser51) (119A11) Rabbit mAb #3597HeLaThapsigargin (300 nM for 30 min)
Phospho-eIF2α (Ser51) Antibody #9721HeLaThapsigargin (300 nM for 30 min)
Phospho-eIF4B (Ser406) (D1C10) Rabbit mAb #8151MCF7Serum-starve overnight, +hIGF-1 (100 ng/ml for 10 min)p70 S6 Kinase MCF7 Control Cell Extracts #34499
Phospho-eIF4B (Ser406) Antibody #5399MCF7Serum-starve overnight, +hIGF-1 (100 ng/ml for 10 min)p70 S6 Kinase MCF7 Control Cell Extracts #34499
Phospho-eIF4B (Ser422) Antibody #3591MCF7Serum-starve overnight, +hIGF-1 (100 ng/ml for 10 min)p70 S6 Kinase MCF7 Control Cell Extracts #34499
Phospho-eIF4E (Ser209) Antibody #9741MCF7Serum-starve overnight, +hIGF-1 (100 ng/ml for 10 min)p70 S6 Kinase MCF7 Control Cell Extracts #34499
Phospho-eIF4G (Ser1108) Antibody #2441MCF7Serum-starve overnight, +hIGF-1 (100 ng/ml for 10 min)p70 S6 Kinase MCF7 Control Cell Extracts #34499
Phospho-Elk-1 (Ser383) (2B1) Mouse mAb #9186recombinant proteinGST-Elk1 fusion protein phosphorylated in vitro by Erk1/2Elk-1 Control Proteins #9183
Phospho-Elk-1 (Ser383) Antibody #9181recombinant proteinGST-Elk1 fusion protein phosphorylated in vitro by Erk1/2Elk-1 Control Proteins #9183
Phospho-eNOS (Ser113) Antibody #9575BAECUntreated
Phospho-eNOS (Thr495) Antibody #9574BAECUntreated, 3 days post-confluence
Phospho-eNOS (Ser1177) (C9C3) Rabbit mAb #9570BAECVEGF (50 ng/ml for 5 min)
Phospho-eNOS (Ser1177) Antibody #9571BAECVEGF (50 ng/ml for 5 min)
Phospho-ENSA (Ser67)/ARPP19 (Ser62) Antibody #5240HeLaThymidine (2 mM for 16 hr) followed by Nocadazole (100 ng/ml for 24 hr)
Phospho-EphA2 (Tyr588) (D7X2L) Rabbit mAb #12677SNB19EphrinA1-Fc ligand-treated (100 ng/ml for 5 min)
Phospho-EphA2 (Tyr594) Antibody #3970Transfected†Transfect cells with construct overexpressing EphA2
Phospho-EphA2 (Tyr772) Antibody #8244SNB19EphrinA1-Fc ligand (100 ng/ml for 5 min)
Phospho-EphA2 (Ser897) (D9A1) Rabbit mAb #6347SNB19Serum
Phospho-EphA3 (Tyr779) (D10H1) Rabbit mAb #8862SNB19EphrinA5-Fc ligand-treated (100 ng/ml for 5 min)
Phospho-Ephrin B (Tyr324/329) Antibody #3481Transfected†NIH/3T3 cells stably expressing Ephrin B1, stimulated with its cognate receptor EphB2-Fc
Phospho-Estrogen Receptor α (Ser104/106) Antibody #2517MCF7Serum-starve overnight, hEGF (100 ng/ml) plus estradiol (100 nM for 30 min)
Phospho-Estrogen Receptor α (Ser118) (16J4) Mouse mAb #2511MCF7Serum-starve overnight, hEGF (100 ng/ml) plus estradiol (100 nM for 30 min)
Phospho-Estrogen Receptor α (Ser167) (D1A3) Rabbit mAb #5587MCF7Estrodiol/hEGF (100 nM each, together for 30 min)
Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® Rabbit mAb #4370JurkatTPA (200 nM for 10 min)p44/42 MAPK (Erk1/2) Control Cell Extracts #9194
Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® Rabbit mAb (HRP Conjugate) #8544JurkatTPA (200 nM for 10 min)p44/42 MAPK (Erk1/2) Control Cell Extracts #9194
Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (197G2) Rabbit mAb (HRP Conjugate) #14474NIH/3T3untreated (-) or treated with TPA #4174 (200 nM, 10 min; +)
Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (197G2) Rabbit mAb #4377JurkatTPA (200 nM for 10 min)p44/42 MAPK (Erk1/2) Control Cell Extracts #9194
Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (20G11) Rabbit mAb #4376JurkatTPA (200 nM for 10 min)p44/42 MAPK (Erk1/2) Control Cell Extracts #9194
Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (197G2) Rabbit mAb (PE Conjugate) #14095N/A
Phospho-p44/42 MAPK (Erk1) (Tyr204)/(Erk2) (Tyr187) (D1H6G) Mouse mAb #5726HeLaSerum-starve overnight, TPA (200 nM for 15 min)MEK 1/2 Control Cell Extracts #9160
Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (E10) Mouse mAb #9106JurkatTPA (200 nM for 10 min)p44/42 MAPK (Erk1/2) Control Cell Extracts #9194
Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) Antibody #9101JurkatTPA (200 nM for 10 min)p44/42 MAPK (Erk1/2) Control Cell Extracts #9194
Phospho-Erk5 (Thr218/Tyr220) Antibody #3371NIH/3T3Serum-starve overnight, PDGF (100 ng/ml for 5 min); IP with ERK5 Antibody prior to western blot
Phospho-Etk (Tyr40) Antibody #3211JurkatSerum-starve overnight, anti-CD3 (1 μg/ml) plus anti-CD28 (0.5 μg/ml for 10 min)
Phospho-Ezrin (Tyr353) (D66D3) Rabbit mAb #5484A-431Serum-starve, hEGF (100 ng/ml for 2 min)
Phospho-Ezrin (Tyr353) Antibody #3144A-431Serum-starve overnight, hEGF (100 ng/ml for 10 min)
Phospho-Ezrin (Thr567)/Radixin (Thr564)/Moesin (Thr558) (48G2) Rabbit mAb #3726PC-12, MCF7, IGROV-1, COS-7
Phospho-Ezrin (Thr567)/Radixin (Thr564)/Moesin (Thr558) Antibody #3141C2C12, NIH/3T3, HeLaUntreated
Phospho-FADD (Ser194) Antibody (Human Specific) #2781JurkatInhibit with hydroxyurea (4.0 mM for 24 hr); activate with Nocadazole (1.0 μg/ml for 24 hr)
Phospho-FAK (Tyr397) (D20B1) Rabbit mAb #8556A549Untreated
Phospho-FAK (Tyr397) Antibody #3283A549Untreated
Phospho-FAK (Tyr576/577) Antibody #3281A549Untreated
Phospho-FAK (Tyr925) Antibody #3284A549Untreated
Phospho-FGF Receptor (Tyr653/654) (55H2) Mouse mAb #3476A204Serum-starve overnight, hFGF basic/FGF2 (100 ng/ml for 10 min)
Phospho-FGF Receptor (Tyr653/654) Antibody #3471A204Serum-starve overnight, hFGF basic/FGF2 (100 ng/ml for 10 min)
Phospho-FGF Receptor 1 (Tyr766) (1E5) Rabbit mAb #2544Transfected†Transfect with a construct overexpressing FGFR1
Phospho-Filamin A (Ser2152) Antibody #4761293Untreated
Phospho-FLT3 (Tyr589/591) (30D4) Rabbit mAb #3464SEMUntreated
Phospho-FLT3 (Tyr591) (33G6) Rabbit mAb #3474SEMUntreated
Phospho-FLT3 (Tyr591) (54H1) Mouse mAb #3466Transfected†Transfect cells with a FLT3 construct
Phospho-FLT3 (Tyr591) Antibody #3461SEMUntreated
Phospho-FLT3 (Tyr842) (10A8) Rabbit mAb #4577SEMUntreated
Phospho-FLT3 (Tyr969) (C24D9) Rabbit mAb #3463SEMUntreated
Phospho-c-Fos (Ser32) (D82C12) XP® Rabbit mAb #5348HeLaSerum-starve overnight, TPA (200 nM for 4 hr)
Phospho-FoxM1 (Ser35) Antibody #14170ACHNNocadazole (100 ng/ml for 18 hr)
Phospho-FoxM1 (Thr600) (D9M6G) Rabbit mAb #14655HeLaNocadazole (100 ng/ml for 16 hr)
Phospho-FoxO1 (Ser256) Antibody #9461HeLaSerum-starve overnight, 20% serum for 30 min
Phospho-FoxO1 (Ser319) Antibody #2486HeLaSerum-starve overnight, 20% serum for 30 min
Phospho-FoxO1 (Thr24)/FoxO3a (Thr32)/FoxO4 (Thr28) (4G6) Rabbit mAb #2599Any cell lineTransfected protein FoxO1, FoxO3a or FoxO4
Phospho-FoxO1 (Thr24)/FoxO3a (Thr32) Antibody #9464HeLaSerum-starve overnight, 20% serum for 30 min
Phospho-FoxO3a (Ser7) Antibody #14724293T
Phospho-FoxO3a (Ser253) (D18H8) Rabbit mAb #13129293T
Phospho-FoxO3a (Ser253) Antibody #9466HeLaSerum-starve overnight, 20% serum for 30 min
Phospho-FoxO3a (Ser294) Antibody #5538C2C12H2O2 treated
Phospho-FoxO3a (Ser318/321) Antibody #9465HeLaSerum-starve overnight, 20% serum for 30 min
Phospho-FoxO3a (Ser413) (D77C9) Rabbit mAb #8174SH-SY5YMG-132-treated (10 μM for 4 hr)
Phospho-FoxO4 (Ser193) Antibody #9471HeLaSerum-starve overnight, 20% serum for 30 min
Phospho-FRA1 (Ser265) (D22B1) Rabbit mAb #5841HeLaSerum-starve overnight, TPA (200 nM for 4 hr)
Phospho-FRA1 (Ser265) Antibody #3880HeLaSerum-starve overnight, TPA (200 nM for 4 hr)
Phospho-FRS2-α (Tyr196) Antibody #3864NIH/3T3Serum-starve overnight, hFGF basic/FGF2 (100 ng/ml for 10 min)
Phospho-FRS2-α (Tyr436) Antibody #3861NIH/3T3Serum-starve overnight, hFGF basic/FGF2 (100 ng/ml for 10 min)
Phospho-Gab1 (Tyr307) Antibody #3234Hep G2Serum-starve overnight, HGF (40 ng/ml for 10 min)
Phospho-Gab1 (Tyr627) (C32H2) Rabbit mAb #3233T-47DSerum-starve overnight, neuregulin (100 ng/ml for 15 min)
Phospho-Gab1 (Tyr627) Antibody #3231H-4-II-ESerum-starve overnight, HGF (40 ng/ml for 10 min)
Phospho-Gab1 (Tyr659) Antibody #12745T47DSerum starve overnight, treated with neuregulin (100 ng/ml 10 min)
Phospho-Gab1 (Tyr659) Antibody #12745T-47DNeuregulin (100 ng/ml for 15 min)
Phospho-Gab2 (Ser159) Antibody #3884H-4-II-ESerum-starve overnight, HGF (40 ng/ml for 10 min)
Phospho-Gab2 (Tyr452) (C33G1) Rabbit mAb #3882T-47DSerum-starve overnight, HGF (40 ng/ml for 10 min)
Phospho-Gab2 (Tyr452) Antibody #3881H-4-II-ESerum-starve overnight, HGF (40 ng/ml for 10 min)
Phospho-GEF-H1 (Ser886) (E1L6D) Rabbit mAb #14143293T, HT-29
Cleaved GGA3 (Asp313) (D16H3) Rabbit mAb #8068HeLaSerum-starve overnight, Staurosporine (1µM for 3 hr)
Phospho-GIT2 (Tyr392) (D8N9A) Rabbit mAb #11873U-2 OS
Phospho-GIT2 (Tyr592) (D6L1J) Rabbit mAb #13167HeLa
Phospho-Glucocorticoid Receptor (Ser211) Antibody #4161A549Serum-starve overnight, dexamethasone (100 nM for 1 hr)
Phospho-AMPA Receptor (GluR 1) (Ser845) (D10G5) Rabbit mAb #8084Rat brain, mouse brain
Phospho-AMPA Receptor (GluR 2) (Tyr869/Tyr873/Tyr876) Antibody #3921Rat brainischemia and reperfusion
Phospho-AMPA Receptor (GluR 2) (Tyr876) Antibody #4027Rat brainischemia and reperfusion
Phospho-Glycogen Synthase (Ser641) Antibody #38913T3Untreated
Phospho-GRB10 (Ser476) (D4E6) Rabbit mAb #11817NIH3T3Serum starve overnight, treated with insulin (100 nM, 5 min) or hIGF-1 #8917 (100 ng/ml for 5 min),
Phospho-GSK-3α/β (Ser21/9) (D17D2) Rabbit mAb #8566NIH/3T3PDGF (100 μg/ml for 5 min)
Phospho-GSK-3α/β (Ser21/9) (37F11) Rabbit mAb (GSK-3α Preferred) #9327NIH/3T3Serum-starve overnight, PDGF (100 ng/ml for 30 min)
Phospho-GSK-3α/β (Ser21/9) Antibody #9331NIH/3T3Serum-starve overnight, PDGF (100 ng/ml for 30 min)
Phospho-GSK-3α (Ser21) (36E9) Rabbit mAb #9316NIH/3T3Serum-starve overnight, PDGF (100 ng/ml for 30 min)
Phospho-GSK-3α (Ser21) (D1G2) Rabbit mAb #8452NIH/3T3PDGF (100 μg/ml for 5 min)
Phospho-GSK-3α (Ser21) (46H12) Mouse mAb #9337NIH/3T3Serum-starve overnight, PDGF (100 ng/ml for 30 min)
Phospho-GSK-3β (Ser9) (D85E12) XP® Rabbit mAb #5558NIH/3T3PDGF (100 μg/ml for 5 min)
Phospho-GSK-3β (Ser9) (5B3) Rabbit mAb #9323NIH/3T3Serum-starve overnight, PDGF (100 ng/ml for 30 min)
Phospho-GSK-3-beta (Ser9) (D3A4) Rabbit mAb #9322NIH/3T3Serum-starve overnight, PDGF (100 ng/ml for 30 min)
Phospho-GSK-3β (Ser9) (D2Y9Y) Mouse mAb #14630PC-3
Phospho-GSK-3β (Ser9) Antibody #9336NIH/3T3Serum-starve overnight, PDGF (100 ng/ml for 30 min)
Phospho-GSK-3β (Thr390) Antibody #3548HeLaPaclitaxel (100 nM/ml for 20 hr)
Phospho-HDAC4 (Ser246)/HDAC5 (Ser259)/HDAC7 (Ser155) (D27B5) Rabbit mAb #3443HeLaSerum-starve overnight, Pervanadate (1 mM for 15 min)
Phospho-HDAC4 (Ser632)/HDAC5 (Ser498)/HDAC7 (Ser486) Antibody #3424HeLaSerum-starve overnight, Pervanadate (1 mM for 15 min)
Phospho-HDAC3 (Ser424) Antibody #3815NIH/3T3Untreated
Phospho-HER2/ErbB2 (Tyr877) Antibody #2241MCF7Serum-starve overnight, neuregulin (100 ng/ml for 5 min)
Phospho-HER2/ErbB2 (Tyr1196) (D66B7) Rabbit mAb #6942MCF7Serum-starve overnight, neuregulin (100 ng/ml for 5 min)
Phospho-HER2/ErbB2 (Tyr1221/1222) (6B12) Rabbit mAb #2243MCF7Serum-starve overnight, neuregulin (100 ng/ml for 5 min)
Phospho-HER2/ErbB2 (Tyr1221/1222) Antibody #2249MCF7Serum-starve overnight, neuregulin (100 ng/ml for 5 min)
Phospho-HER2/ErbB2 (Tyr1248) Antibody #2247MCF7Serum-starve overnight, neuregulin (100 ng/ml for 5 min)
Phospho-HER3/ErbB3 (Tyr1197) (C56E4) Rabbit mAb #4561MCF7Serum-starve overnight, neuregulin (100 ng/ml for 5 min)
Phospho-HER3/ErbB3 (Tyr1222) (50C2) Rabbit mAb #4784MCF7Serum-starve overnight, neuregulin (100 ng/ml for 5 min)
Phospho-HER3/ErbB3 (Tyr1289) (D1B5) Rabbit mAb #2842MCF7NRG (100 ng/ml for 5 min)
Phospho-HER3/ErbB3 (Tyr1289) (21D3) Rabbit mAb #4791MCF7Serum-starve overnight, neuregulin (100 ng/ml for 5 min)
Phospho-HER3/ErbB3 (Tyr1328) (E1J1T) Rabbit mAb #14525T47DSerum starve overnight, treated with neuregulin (100 ng/ml 5 min)
Phospho-HER3/ErbB3 (Tyr1328) (E1J1T) Rabbit mAb #14525T-47DHuman Neuregulin-1 (hNRG-1) #5218 (100 ng/ml for 5 min)
Phospho-HER4/ErbB4 (Tyr984) Antibody #3790MCF7Serum-starve overnight, neuregulin (100 ng/ml for 5 min)
Phospho-HER4/ErbB4 (Tyr1284) (21A9) Rabbit mAb #4757MCF7Serum-starve overnight, neuregulin (100 ng/ml for 5 min)
Acetyl-Histone H2A (Lys5) Antibody #2576NIH/3T3TSA (400 nM overnight)
Phospho-Histone H2A.X (Ser139) (20E3) Rabbit mAb #9718293UV‡ (100 mJ/cm2), 2 hr recovery before lysing
Phospho-Histone H2A.X (Ser139) Antibody #2577293UV‡ (100 mJ/cm2), 2 hr recovery before lysing
Phospho-Histone H2A.X (Ser139/Tyr142) Antibody #5438293UV‡ (100 mJ/cm2), 2 hr recovery before lysing
Phospho-Histone H2B (Ser14) (D67H2) Rabbit mAb #6959293UV‡ (100 mJ/cm2), 2 hr recovery before lysing
Acetyl-Histone H2B (Lys5) (D5H1S) XP® Rabbit mAb #12799HeLaTSA (400 nM overnight)
Acetyl-Histone H2B (Lys5) Antibody #2574NIH/3T3, HeLaTSA (400 nM overnight)
Acetyl-Histone H2B (Lys12) (D7H4) Rabbit mAb #9861NIH/3T3, HeLaTSA (400 nM overnight)
Acetyl-Histone H2B (Lys12) Antibody #5410NIH/3T3, HeLATSA (400 nM overnight)
Acetyl-Histone H2B (Lys15) (D8H1) XP® Rabbit mAb #9083NIH/3T3, HeLaTSA (400 nM overnight)
Acetyl-Histone H2B (Lys20) Antibody #2571NIH/3T3, HeLaTSA (400 nM overnight)
Phospho-Histone H3 (Thr3) (D5G1I) Rabbit mAb #13576HeLaSerum-starve overnight, add 20% serum for 15 min, then Calyculin A (100 nM for 15 min)
Phospho-Histone H3 (Thr3) Antibody #9714NIH/3T3, HeLaSerum-starve overnight, add 20% serum for 15 min, then Calyculin A (100 nM for 15 min)
Phospho-Histone H3 (Ser10) (D2C8) XP® Rabbit mAb #3377NIH/3T3, HeLaSerum-starve overnight, add 20% serum for 15 min, then Calyculin A (100 nM for 15 min)
Phospho-Histone H3 (Ser10) (6G3) Mouse mAb #9706NIH/3T3, HeLaSerum-starve overnight, add 20% serum for 15 min, then Calyculin A (100 nM for 15 min)
Phospho-Histone H3 (Ser10) Antibody #9701NIH/3T3, HeLaSerum-starve overnight, add 20% serum for 15 min, then Calyculin A (100 nM for 15 min)
Phospho-Histone H3 (Thr11) (C2A6) Rabbit mAb #9767HeLaNocadazole (100 ng/ml 24 hr)
Phospho-Histone H3 (Thr11) Antibody #9764HeLaNocadazole (100 ng/ml 24 hr)
Phospho-Histone H3 (Ser28) Antibody #9713HeLaNocadazole (100 ng/ml 24 hr)
Methyl-Histone H3 (Arg2) Antibody #9707HeLaTSA (400 nM overnight)
Mono-Methyl-Histone H3 (Lys4) (D1A9) XP® Rabbit mAb #5326NIH/3T3, HeLaUntreated
Mono-Methyl-Histone H3 (Lys4) Antibody #9723NIH/3T3, HeLaUntreated
Pan-Methyl-Histone H3 (Lys9) (D54) XP® Rabbit mAb (HRP Conjugate) #6951NIH/3T3, HeLaUntreated
Pan-Methyl-Histone H3 (Lys9) (D54) XP® Rabbit mAb #4473NIH/3T3, HeLaUntreated
Mono-Methyl-Histone H3 (Lys9) (D1P5R) Rabbit mAb #14186HeLa, NIH/3T3Untreated
Mono-Methyl-Histone H3 (Lys36) (D9J1D) Rabbit mAb #14111HeLa, NIH/3T3Untreated
Mono-Methyl-Histone H3 (Lys79) (D5X1S) Rabbit mAb #12522HeLa, NIH/3T3Untreated
Mono-Methyl-Histone H3 (Lys79) Antibody #9398NIH/3T3, HeLaUntreated
Tri-Methyl-Histone H3 (Lys4) (C42D8) Rabbit mAb (PE Conjugate) #62255NIH/3T3, HeLaUntreated
Tri-Methyl-Histone H3 (Lys4) (C42D8) Rabbit mAb #9751NIH/3T3, HeLaUntreated
Tri-Methyl-Histone H3 (Lys4) Antibody #9727NIH/3T3, HeLaUntreated
Tri-Methyl-Histone H3 (Lys9) (D4W1U) Rabbit mAb #13969NIH/3T3, HeLaUntreated
Di/Tri-Methyl-Histone H3 (Lys9) (6F12) Mouse mAb #5327NIH/3T3, HeLaUntreated
Tri-Methyl-Histone H3 (Lys27) (C36B11) Rabbit mAb (PE Conjugate) #40724NIH/3T3, HeLaUntreated
Tri-Methyl-Histone H3 (Lys27) (C36B11) Rabbit mAb #9733NIH/3T3, HeLaUntreated
Tri-Methyl-Histone H3 (Lys36) (D5A7) XP® Rabbit mAb #4909NIH/3T3, HeLaUntreated
Tri-Methyl-Histone H3 (Lys36) Antibody #9763NIH/3T3, HeLaUntreated
Tri-Methyl-Histone H3 (Lys79) Antibody #4260NIH/3T3, HeLaUntreated
Acetyl-Histone H3 (Lys9) (C5B11) Rabbit mAb (HRP Conjugate) #14052HeLaTSA (400 nM overnight)
Acetyl-Histone H3 (Lys9) (C5B11) Rabbit mAb #9649HeLaTSA (400 nM overnight)
Acetyl-Histone H3 (Lys9/Lys14) Antibody #9677HeLaTSA (400 nM overnight)
Acetyl- and Phospho-Histone H3 (Lys9/Ser10) Antibody #9711NIH/3T3Serum-starve plus 400 nM TSA overnight, add 20% serum for 15 min, then 100 nM Calyculin A for 15 min
Acetyl-Histone H3 (Lys14) (D4B9) Rabbit mAb #7627HeLaTSA (400 nM overnight)
Acetyl-Histone H3 (Lys14) Antibody (ChIP Formulated) #5275HeLaTSA (400 nM overnight)
Acetyl-Histone H3 (Lys18) (D8Z5H) Rabbit mAb #13998HeLaTSA (400 nM overnight)
Acetyl-Histone H3 (Lys18) Antibody #9675HeLaTSA (400 nM overnight)
Acetyl-Histone H3 (Lys23) (D6Y7M) Rabbit mAb #14932HeLaTSA (400 nM overnight)
Acetyl-Histone H3 (Lys23) Antibody #8848HeLaTSA (400 nM overnight)
Acetyl-Histone H3 (Lys27) (D5E4) XP® Rabbit mAb (HRP Conjugate) #14056HeLaTSA (400 nM overnight)
Acetyl-Histone H3 (Lys27) (D5E4) XP® Rabbit mAb #8173HeLaTSA (400 nM overnight)
Acetyl-Histone H3 (Lys27) Antibody #4353HeLaTSA (400 nM overnight)
Acetyl-Histone H3 (Lys36) Antibody #11885HeLaTSA (400 nM overnight)
Acetyl-Histone H3 (Lys56) Antibody #4243HeLaTSA (400 nM overnight)
Cleaved Histone H3 (Thr22) (D7J2K) Rabbit mAb #12576NTERA-2Retinoic acid (10 µM for 3 days)
Cleaved Histone H3 (Thr22) Antibody #7469NTERA-2Retinoic acid (10 µM for 3 days)
Di-Methyl-Histone H3 (Lys4) (C64G9) Rabbit mAb #9725NIH/3T3Untreated
Di-Methyl-Histone H3 (Lys9) (D85B4) XP® Rabbit mAb #4658NIH/3T3Untreated
Di-Methyl-Histone H3 (Lys9) Antibody #9753NIH/3T3Untreated
Di-Methyl-Histone H3 (Lys27) (D18C8) XP® Rabbit mAb #9728NIH/3T3Untreated
Di-Methyl-Histone H3 (Lys27) (D18C8) XP® Rabbit mAb (HRP Conjugate) #14040NIH/3T3Untreated
Di-Methyl-Histone H3 (Lys36) (C75H12) Rabbit mAb #2901NIH/3T3Untreated
Di-Methyl-Histone H3 (Lys79) (D15E8) XP® Rabbit mAb #5427NIH/3T3Untreated
Mono-Methyl-Histone H4 (Lys20) Antibody #9724NIH/3T3Untreated
Tri-Methyl-Histone H4 (Lys20) (D84D2) Rabbit mAb #5737NIH/3T3Untreated
Acetyl-Histone H4 (Lys5) (D12B3) Rabbit mAb #8647HeLaTSA (400 nM overnight)
Acetyl-Histone H4 (Lys5) Antibody #9672HeLaTSA (400 nM overnight)
Acetyl-Histone H4 (Lys8) Antibody #2594HeLaTSA (400 nM overnight)
Acetyl-Histone H4 (Lys12) (D2W6O) Rabbit mAb #13944HeLaTSA (400 nM overnight)
Acetyl-Histone H4 (Lys12) Antibody #2591HeLaTSA (400 nM overnight)
Acetyl-Histone H4 (Lys16) (E2B8W) Rabbit mAb #13534HeLaTSA (400 nM overnight)
Di-Methyl-Histone H4 (Lys20) Antibody #9759NIH/3T3Untreated
Phospho-HP1γ (Ser83) Antibody #2600HeLaIBMX (30 µM) plus Forskolin (500 µM for 30 min)
Phospho-HS1 (Tyr397) (D12C1) XP® Rabbit mAb #8714RamosSerum-starve overnight, anti-IgM (12 μg/mL for 10 min)
Phospho-HS1 (Tyr397) Antibody #4507RamosSerum-starve overnight, IgM (12 µg/ml for 10 min)
Phospho-HSL (Ser563) Antibody #4139differentiated 3T3-L1Isoproterenol (10 µM for 20 min)
Phospho-HSL (Ser565) Antibody #4137differentiated 3T3-L1Isoproterenol (10 µM for 20 min)
Phospho-HSL (Ser660) Antibody #4126differentiated 3T3-L1Isoproterenol (10 µM for 20 min)
Phospho-HSP27 (Ser15) Antibody #2404HeLaSerum-starve overnight, UV‡ (40 mJ/cm2), 30 min recovery
Phospho-HSP27 (Ser78) Antibody #2405HeLaSerum-starve overnight, UV‡ (40 mJ/cm2), 30 min recovery
Phospho-HSP27 (Ser82) (D1H2) XP® Rabbit mAb #9709HeLaAnisomycin (25 μg/mL for 30 min)
Phospho-HSP27 (Ser82) Antibody #2401HeLaSerum-starve overnight, UV‡ (40 mJ/cm2), 30 min recovery
Phospho-HSP27 (Ser82) Antibody II #2406HeLaSerum-starve overnight, UV‡ (40 mJ/cm2), 30 min recovery
Phospho-HSP90α (Thr5/7) Antibody #3488HeLaSerum-starve overnight, UV‡ (40 mJ/cm2), 30 min recovery
Cleaved Drosophila ICE (drICE) (Asp230) Antibody #9478S2Cycloheximide (10 µM for 5 hr) or Actinomycin D (1 µM for 5 hr)
Phospho-IGF-I Receptor β (Tyr980) (C14A11) Rabbit mAb #4568293Serum-starve overnight, hIGF-I (100 ng/ml for 5 min)
Phospho-IGF-I Receptor β (Tyr1131)/Insulin Receptor β (Tyr1146) Antibody #3021293Serum-starve overnight, hIGF-I (100 ng/ml for 5 min)
Phospho-IGF-I Receptor β (Tyr1316) Antibody #6113293Serum-starve overnight, hIGF-I (100 ng/ml for 5 min)
Phospho-IGF-I Receptor β (Tyr1135) (DA7A8) Rabbit mAb #3918293Serum-starve overnight, hIGF-I (100 ng/ml for 5 min)
Phospho-IGF-I Receptor β (Tyr1135/1136)/Insulin Receptor β (Tyr1150/1151) (19H7) Rabbit mAb #3024293Serum-starve overnight, hIGF-I (100 ng/ml for 5 min)
Phospho-IκBα (Ser32) (14D4) Rabbit mAb #2859HeLahTNF-α (20 ng/ml for 5 min)NF-κB Control Cell Extracts #9243
Phospho-IκBα (Ser32/36) (5A5) Mouse mAb #9246HeLahTNF-α (20 ng/ml for 5 min)NF-κB Control Cell Extracts #9243
Phospho-IκBβ (Thr19/Ser23) Antibody (Human Specific) #4921HeLahTNF-α (20 ng/ml) plus Calyculin A (50 nM for 5 min)
Phospho-IκBε (Ser18/22) Antibody #4924HeLahTNF-α (20 ng/ml) plus Calyculin A (50 nM for 5 min)NF-κB Control Cell Extracts #9243
Phospho-IKKα (Ser176)/IKKβ (Ser177) (C84E11) Rabbit mAb #2078HelahTNF-α (20 ng/ml for 5 min)NF-κB Control Cell Extracts #9243
Phospho-IKKα/β (Ser176/180) Antibody II #2694HeLahTNF-α (20 ng/ml for 10 min)NF-κB Control Cell Extracts #9243
Phospho-IKKα/β (Ser176/180) (16A6) Rabbit mAb #2697HeLahTNF-α (20 ng/ml for 10 min)NF-κB Control Cell Extracts #9243
Phospho-IKKα/β (Ser176/180) (16A6) Rabbit mAb (PE Conjugate) #14938HeLahTNF-α (20 ng/ml for 10 min)
Phospho-IKKε (Ser172) (D1B7) Rabbit mAb #8766THP-1TPA (80 nM overnight), LPS (1 μg/ml for 15 min)
Phospho-IKKγ (Ser376) Antibody #2689HeLahTNF-α (20 ng/ml for 10 min)NF-κB Control Cell Extracts #9243
Phospho-Insulin Receptor β (Tyr1345) (14A4) Rabbit mAb #3026Transfected†Transfect with IR/IRS-1, serum-starve overnight, insulin (100 nM for 5 min)
Phospho-Insulin Receptor β (Tyr1361) (84B2) Rabbit mAb #3023Transfected†Transfect with IR/IRS-1, serum-starve overnight, insulin (100 nM for 5 min)
Phospho-IP3 Receptor (Ser1756) (D10E3) Rabbit mAb #8548Mouse brain
Phospho-IP3 Receptor (Ser1756) Antibody #3760Mouse brain, rat brainUntreated
Phospho-IRAK4 (Thr345/Ser346) (D6D7) Rabbit mAb #11927KARPAS-299hIL-1β (50 ng/ml for 15 min)
Phospho-IRF-3 (Ser396) (D6O1M) Rabbit mAb (PE Conjugate) #83611RAW264.7LPS (1 μg/ml for 60 min)
Phospho-IRF-3 (Ser396) (4D4G) Rabbit mAb #4947RAW264.7LPS (1 μg/ml for 60 min)
Phospho-IRF-3 (Ser396) (D6O1M) Rabbit mAb #29047RAW264.7LPS (1 μg/ml for 60 min)
Phospho-IRF-7 (Ser471/472) Antibody #5184Transfected†IRF-7 overexpression (transfected)
Phospho-IRF-7 (Ser477) (D7E1W) Rabbit mAb #12390HT-29hIFN-α1 (10 ng/ml, overnight) and poly(I:C) (2.5 μg/ml for 7 hr)
Phospho-IRF-7 (Ser437/438) Antibody (Mouse Specific) #14767RAW264.7LPS (1 μg/ml overnight)
Phospho-IRS-1 (Ser302) (34C7) Rabbit mAb #2491Transfected†Transfect with IR/IRS-1, serum-starve overnight, insulin (100 nM for 15 min)
Phospho-IRS-1 (Ser302) Antibody #2384MCF7Serum-starve overnight, insulin (100 nM for 5 min)
Phospho-IRS-1 (Ser307) Antibody #2381MCF7Serum-starve overnight, insulin (100 nM for 5 min)
Phospho-IRS-1 (Ser318) (D51C3) Rabbit mAb #5610MCF7Serum-starve overnight, insulin (100 nM for 5 min)
Phospho-IRS-1 (Ser332/336) Antibody #2580Transfected†Transfect with IR/IRS-1, serum-starve overnight, insulin (100 nM for 10 min)
Phospho-IRS-1 (Ser612) (C15H5) Rabbit mAb #3203MCF7Serum-starve overnight, insulin (100 nM for 5 min)
Phospho-IRS-1 (Ser612) (L7B8) Mouse mAb #3193MCF7Serum-starve overnight, insulin (100 nM for 5 min)
Phospho-IRS-1 (Ser612) Antibody #2386mIMCD-3Serum-starve overnight, insulin (100 nM for 5 min)
Phospho-IRS-1 (Ser636/639) Antibody #2388MCF7Serum-starve overnight, insulin (100 nM for 5 min)
Phospho-IRS-1 (Ser789) Antibody #2389Transfected†Transfect with IR/IRS-1, serum-starve overnight, insulin (100 nM for 10 min)
Phospho-IRS-1 (Tyr895) Antibody #3070Transfected†Transfect with IR/IRS-1, serum-starve overnight, insulin (100 nM for 10 min)
Phospho-IRS-1 (Ser1101) Antibody #2385Differentiated C2C12 (myotubes)Serum-starve overnight, insulin (100 nM for 5 min)
Phospho-IRS-1 (Tyr1222) Antibody #3066Transfected†Transfect with IR/IRS-1, serum-starve overnight, insulin (100 nM for 10 min)
Phospho-Jak1 (Tyr1022/1023) Antibody #3331HT-29Serum-starve overnight, IL-4 (100 ng/ml for 5–10 min)
Phospho-Jak2 (Tyr221) Antibody #3774CTLL-2Untreated
Phospho-Jak2 (Tyr1008) (D4A8) Rabbit mAb #8082UT7Serum-starve overnight, hGM-CSF (25 ng/ml for 15 min); hGM-CSF (2 ng/ml) should be included during growth and serum-starvation
Phospho-Jak2 (Tyr1007) (D15E2) Rabbit mAb #4406UT7Serum-starve overnight, hGM-CSF (25 ng/ml for 15 min); hGM-CSF (2 ng/ml) should be included during growth and serum-starvation
Phospho-Jak2 (Tyr1007/1008) (C80C3) Rabbit mAb #3776UT7Serum-starve overnight, hGM-CSF (25 ng/ml for 15 min); hGM-CSF (2 ng/ml) should be included during growth and serum-starvation
Phospho-Jak2 (Tyr1007/1008) Antibody #3771UT7Serum-starve overnight, hGM-CSF (25 ng/ml for 15 min); hGM-CSF (2 ng/ml) should be included during growth and serum-starvation
Phospho-Jak3 (Tyr980/981) (D44E3) Rabbit mAb #5031NK-92Cytokine-starve overnight, IL-2 (10 ng/ml for 15 min); IL-2 (5 ng/ml) should be included during growth
Phospho-c-Jun (Ser63) (54B3) Rabbit mAb #2361NIH/3T3UV‡ (40 mJ/cm2), 30 min recovery timec-Jun Control Cell Extracts #9263
Phospho-c-Jun (Ser63) II Antibody #9261NIH/3T3UV‡ (40 mJ/cm2), 30 min recovery timec-Jun Control Cell Extracts #9263
Phospho-c-Jun (Ser73) (D47G9) XP® Rabbit mAb #3270NIH/3T3UV‡ (40 mJ/cm2), 30 min recovery timec-Jun Control Cell Extracts #9263
Phospho-c-Jun (Ser73) Antibody #9164NIH/3T3UV‡ (40 mJ/cm2), 30 min recovery timec-Jun Control Cell Extracts #9263
Phospho-c-Jun (Thr91) Antibody #2303NIH/3T3UV‡ (40 mJ/cm2), 30 min recovery timec-Jun Control Cell Extracts #9263
Phospho-c-Jun (Thr93) Antibody #2993NIH/3T3Untreated; site is constitutively phosphorylatedc-Jun Control Cell Extracts #9263
Phospho-c-Jun (Ser243) Antibody #2994NIH/3T3UV‡ (40 mJ/cm2), 30 min recovery timec-Jun Control Cell Extracts #9263
Phospho-JunB (Thr102/Thr104) (D3C6) Rabbit mAb #8053C6Anisomycin (25 ug/ml for 20 min)p38 MAPK Control Cell Extracts #9213
Phospho-KIF1B (Ser1487) Antibody #11993PC-3
Phospho-c-Kit (Tyr703) (D12E12) Rabbit mAb #3073NCI-H526Serum-starve overnight, hSCF (100 ng/ml for 5 min)
Phospho-c-Kit (Tyr719) Antibody #3391NCI-H526Serum-starve overnight, hSCF (100 ng/ml for 5 min)
Phospho-Keratin 17 (Ser44) Antibody #3519HeLaNocadazole (100 ng/ml for 4 hr)
Phospho-KSR1 (Ser392) Antibody #4951HeLaUntreated; site is constitutively phosphorylated
Cleaved Lamin A (Small Subunit) (30H5) Mouse mAb #2036HeLaSerum-starve overnight, Staurosporine (1.0 μM for 4 hr)
Cleaved Lamin A (Small Subunit) Antibody #2035HeLaSerum-starve overnight, Staurosporine (1.0 μM for 4 hr)
Phospho-Lamin A/C (Ser22) (D2B2E) XP® Rabbit mAb #13448HeLa, NIH/3T3, C6Activate with Paclitaxel (100nM for 20 hr); Inhibit with hydroxyurea (4.0 mM for 20 hr)
Phospho-Lamin A/C (Ser22) Antibody #2026THP-1Nocadazole (1 μg/ml for 20 hr)
Cleaved Lamin A (Asp230) Antibody #2031HeLaSerum-starve overnight, Staurosporine (1.0 μM for 4 hr)
Phospho-LAT (Tyr171) Antibody #3581JurkatAnti-CD3 (10 µg/ml for 2 min)
Phospho-LAT (Tyr191) Antibody #3584JurkatAnti-CD3 (10 µg/ml for 2 min)
Phospho-LATS1 (Thr1079) (D57D3) Rabbit mAb #8654HeLaOkadaic Acid (1 μM for 1 hr)
Phospho-LATS1 (Ser909) Antibody #9157HeLaTPA (200 nM for 30 min)
Phospho-Lck (Tyr505) Antibody #2751JurkatAnti-CD3 (10 µg/ml for 2 min)
Phospho-LCP1 (Tyr28) Antibody #5277MOLT-15Untreated
Phospho-LDHA (Tyr10) Antibody #8176K562Untreated
Phospho-LIMK1 (Thr508)/LIMK2 (Thr505) Antibody #3841Transfected†Transfect with construct overexpressing LIMK1
Phospho-LIN28A (Ser200) Antibody #70690NCCITUntreated
Phospho-LKB1 (Thr189) Antibody #3054Transfected†Transfect with construct overexpressing LKB1
Phospho-LKB1 (Ser334) Antibody #3055Transfected†Transfect with construct overexpressing LKB1
Phospho-LKB1 (Ser428) (C67A3) Rabbit mAb #3482CakiTPA (200 nM for 30 min)
Phospho-5-Lipoxygenase (Ser271) Antibody #3748Transfected†Transfect cells with construct overexpressing 5-lipoxygenase
Phospho-5-Lipoxygenase (Ser663) Antibody #3749Mouse brain, rat brainUntreated
Phospho-LRP6 (Ser1490) Antibody #2568HeLaWnt3a-conditioned media for 5 hr (Conditioned media from L cells transfected with Wnt3a ligand)
Phospho-Lyn (Tyr507) Antibody #2731RamosAnti-IgM (12 μg/ml for 10 min)
Tri-Methyl Lysine Motif [tme-K] (D1L1X) Rabbit mAb #14680HeLaUntreated
Acetylated-Lysine (Ac-K2-100) Rabbit mAb #9814COSTSA (0.4 μM for 18 hr)
Acetylated-Lysine (Ac-K2-100) Rabbit mAb (HRP Conjugate) #6952COSTSA (0.4 µM for 18 hr)
Acetylated-Lysine Mouse mAb (Ac-K-103) #9681COSTSA (0.4 μM for 18 hr)
Acetylated-Lysine Antibody #9441COSTSA (0.4 μM for 18 hr)
Malonyl-Lysine [Mal-K] MultiMab™ Rabbit mAb mix #14942SIRT5 knock-out mouse liver
Phospho-MAP2 (Ser136) Antibody #4541PC-12Positive control: Nocadazole (1 µg/ml for 16 hr); negative control: λ phosphatase (800 U/500 μl for 1 hr)
Phospho-MAP2 (Thr1620/1623) Antibody #4544PC-12Positive control: Nocadazole (1 µg/ml for 16 hr); negative control: λ phosphatase (800 U/500 μl for 1 hr)
Phospho-MAPK Substrates Motif [PXpTP] MultiMab™ Rabbit mAb mix #14378HCT 116Thymidine 2 mM for 18 hr followed by Nocadazole (50 ng/ml for 20 hr)
Phospho-MAPK Substrates Motif [PXpTP] Rabbit mAb #14378HCT 116Thymidine (2 mM for 18 hr) followed by Nocadazole #2190 (50 ng/ml for 20 hr)
Phospho-CDK Substrate [pTPXK] (D9V5N) Rabbit mAb #14371HCT 116Thymidine (2 mM for 18 hr), followed by Nocadazole #2190 (50 ng/ml for 20 hr)
Phospho-CDK Substrate [pTPXK] (D9V5N) Rabbit mAb #14371HCT 116Thymidine (2 mM for 18 hr) followed by Nocadazole #2190 (50 ng/ml for 20 hr)
Phospho-MAPKAPK-2 (Thr222) (9A7) Rabbit mAb #3316HeLaSerum-starve overnight, UV‡ (40 mJ/cm2), 20 min recovery
Phospho-MAPKAPK-2 (Thr334) (27B7) Rabbit mAb #3007HeLaSerum-starve overnight, UV‡ (40 mJ/cm2), 20 min recovery
Phospho-MAPKAPK-2 (Thr334) Antibody #3041HeLaSerum-starve overnight, UV‡ (40 mJ/cm2), 20 min recovery
Phospho-p38 MAPK (Thr180/Tyr182) (D3F9) XP® Rabbit mAb #4511C6Anisomycin (25 μg/ml for 20 min)p38 MAP Kinase Control Cell Extracts #9213
Phospho-p38 MAPK (Thr180/Tyr182) (12F8) Rabbit mAb #4631C6Anisomycin (25 μg/ml for 20 min)p38 MAP Kinase Control Cell Extracts #9213
Phospho-p38 MAPK (Thr180/Tyr182) (3D7) Rabbit mAb #9215C6Anisomycin (25 μg/ml for 20 min)p38 MAP Kinase Control Cell Extracts #9213
Phospho-p38 MAPK (Thr180/Tyr182) (28B10) Mouse mAb #9216C6Anisomycin (25 μg/ml for 20 min)p38 MAP Kinase Control Cell Extracts #9213
Phospho-p38 MAPK (Thr180/Tyr182) Antibody #9211C6Anisomycin (25 μg/ml for 20 min)p38 MAP Kinase Control Cell Extracts #9213
Phospho-MARCKS (Ser159/163) (D13D2) Rabbit mAb #11992HeLa, NIH-3T3, C6
Phospho-MARCKS (Ser152/156) Antibody #2741HeLa, NIH/3T3Serum-starve overnight, TPA (200 nM for 15 min)
Phospho-MARCKS (Ser167/170) (D13E4) XP® Rabbit mAb #8722SK-N-MCTPA (200 nM for 15 min)
Phospho-MARK Family (Activation Loop) Antibody #4836Raji, BaF3Untreated
Phospho-Mcl-1 (Ser64) Antibody #13297HCT 116, KARPAS-299MG-132 (10 µM overnight)
Phospho-Mcl-1 (Ser159/Thr163) Antibody #4579NCI-H929MG-132 (10 μM for 2 hr)
Phospho-Mcl-1 (Thr163) (D5M9D) Rabbit mAb #14765Raji, KARPAS-299Serum-starve overnight, TPA (200 nM for 30 min)
Phospho-MCM2 (Ser139) (D1Z8X) XP® Rabbit mAb #12958Hela, C2C12Untreated
Phospho-MCM2 (Ser139) Antibody #8861HeLa, C2C12Untreated
Phospho-MCM3 (Ser112) (D3S4M) Rabbit mAb #12686HelaNocadazole (100 ng/ml for 24 hr)
Phospho-MDM2 (Ser166) Antibody #3521MCF7hIGF-I (100 ng/ml for 15 min)
Phospho-MEF2A (Ser408) Antibody #9737Transfected†Transfect with construct overexpressing MEF2A
Phospho-MEK1 (Thr286) Antibody #9127HeLaSerum-starve overnight, Nocadazole (0.2 μg/ml for 16 hr)MEK1/2 HeLa Control Cell Extracts #9160
Phospho-MEK1 (Thr292) Antibody #51265A431Untreated; site is constitutively phosphorylated
Phospho-MEK1 (Thr292) Antibody #51265A431Untreated
Phospho-MEK1 (Ser298) Antibody #9128HeLaSerum-starve overnight, TPA (200 nM for 15 min)MEK1/2 HeLa Control Cell Extracts #9160
Phospho-MEK1/2 (Ser217/221) (41G9) Rabbit mAb #9154HeLaSerum-starve overnight, TPA (200 nM for 15 min)MEK1/2 HeLa Control Cell Extracts #9160
Phospho-MEK1/2 (Ser221) (166F8) Rabbit mAb #2338HeLaSerum-starve overnight, TPA (200 nM for 15 min)MEK1/2 HeLa Control Cell Extracts #9160
Phospho-MEK1/2 (Ser217/221) Antibody #9121HeLaSerum-starve overnight, TPA (200 nM for 15 min)MEK1/2 HeLa Control Cell Extracts #9160
Phospho-Merlin (Ser518) (D5A4I) Rabbit mAb #13281PC-3Untreated
Phospho-Merlin (Ser518) Antibody #9163PC-3Untreated
Phospho-Met (Tyr1003) (13D11) Rabbit mAb #3135HCC827Untreated
Phospho-Met (Tyr1234/1235) (D26) XP® Rabbit mAb #3077HCC827Untreated
Phospho-Met (Tyr1234/1235) (3D7) Rabbit mAb #3129H-4-II-ESerum-starve overnight, HGF (40 ng/ml for 10 min)
Phospho-Met (Tyr1234/1235) Antibody #3126H-4-II-ESerum-starve overnight, HGF (40 ng/ml for 10 min)
Phospho-Met (Tyr1349) (130H2) Rabbit mAb #3133H-4-II-ESerum-starve overnight, HGF (40 ng/ml for 10 min)
Phospho-Met (Tyr1349) Antibody #3121H-4-II-ESerum-starve overnight, HGF (40 ng/ml for 10 min)
Symmetric Di-Methyl Arginine Motif [sdme-RG] Rabbit mAb #13222MCF7Adenosine-2', 3'-dialdehyde (AdOx, 100 µM for 24 hr)
Symmetric Di-Methyl Arginine Motif [sdme-RG] Rabbit mAb #13522MCF7Adenosine-2', 3'-dialdehyde (AdOx, 100 µM for 24 hr)
Phospho-MKK3 (Ser189)/MKK6 (Ser207) (22A8) Rabbit mAb #9236NIH/3T3UV‡ (40 mJ/cm2), 30 min recovery timeMKK3/MKK6 Control Cell Extracts #9233
Phospho-MKK3 (Ser189)/MKK6 (Ser207) (D8E9) Rabbit mAb #12280HeLaTPA (200 nM for 15 min)
Phospho-MKK3 (Ser189)/MKK6 (Ser207) Antibody #9231NIH/3T3UV‡ (40 mJ/cm2), 30 min recovery timeMKK3/MKK6 Control Cell Extracts #9233
Phospho-MKK7 (Ser271/Thr275) Antibody #4171U-937Sorbitol (400 mM for 15 min)
Phospho-MLKL (Ser358) (D6H3V) Rabbit mAb #91689HT-29Pre-treat Z-VAD (20 μM for 30 min) followed by addition of CHX (10 μg/ml) and hTNF-α (20 ng/ml) overnight
Phospho-Mnk1 (Thr197/202) Antibody #2111HeLaSerum-starve overnight, 20% serum for 20 min or Anisomycin (25 µg/ml for 30 min)p38 MAP Kinase Control Cell Extracts #9213
Phospho-MOB1 (Thr35) (D2F10) Rabbit mAb #8699MCF7Untreated or Treated with hydrogen peroxide (H202) (2.5 mM for 30 min)
Phospho-MOB1 (Thr12) (D2E3) Rabbit mAb #8843MCF7Untreated or Treated with hydrogen peroxide (H202) (2.5 mM for 30 min)
Phospho-Mre11 (Ser676) Antibody #4859HeLaUV‡ (100 mJ/cm2), 1 hr recovery before lysing
Phospho-MSK1 (Ser360) Antibody #9594Transfected†Transfect with construct overexpressing MSK1
Phospho-MSK1 (Ser376) Antibody #9591293UV‡ (40 mJ/cm2), 30 min recovery timeSAPK/JNK Control Cell Extracts #9253
Phospho-MSK1 (Thr581) Antibody #9595293UV‡ (40 mJ/cm2), 30 min recovery timeSAPK/JNK Control Cell Extracts #9253
Phospho-Mst1 (Thr183)/Mst2 (Thr180) Antibody #3681WEHIStaurosporine (1 µM for 30 min – 3 hr)
Phospho-mTOR (Ser2448) (D9C2) XP® Rabbit mAb #5536MCF7Serum-starve overnight, +hIGF-1 (100 ng/ml for 10 min)p70 S6 Kinase MCF7 Control Cell Extracts #34499
Phospho-mTOR (Ser2448) (49F9) Rabbit mAb (IHC Specific) #2976MCF7Serum-starve overnight, +hIGF-1 (100 ng/ml for 10 min)p70 S6 Kinase MCF7 Control Cell Extracts #34500
Phospho-mTOR (Ser2448) Antibody #2971MCF7Serum-starve overnight, +hIGF-1 (100 ng/ml for 10 min)p70 S6 Kinase MCF7 Control Cell Extracts #34501
Phospho-mTOR (Ser2481) Antibody #2974MCF7Serum-starve overnight, +hIGF-1 (100 ng/ml for 10 min)p70 S6 Kinase MCF7 Control Cell Extracts #34502
Phospho-c-Myc (Ser62) (E1J4K) Rabbit mAb #13748HCT 116MG-132 (10 µM overnight)
Phospho-Myosin IIa (Ser1943) (D7Z7T) Rabbit mAb #14611293T, ZR-75
Phospho-Myosin IIa (Ser1943) Antibody #5026HeLa, A-431, 293TUntreated
Phospho-Myosin Light Chain 2 (Thr18/Ser19) Antibody #3674C2C12Untreated
Phospho-Myosin Light Chain 2 (Ser19) Mouse mAb #3675C2C12Untreated
Phospho-Myosin Light Chain 2 (Ser19) Antibody #3671C2C12Untreated
Phospho-MYPT1 (Ser507) Antibody #3040HeLaSerum-starve overnight, Calyculin A (100 nM for 30 min)
Phospho-MYPT1 (Ser668) Antibody #3048HeLaSerum-starve overnight, Calyculin A (100 nM for 30 min)
Phospho-MYPT1 (Thr696) Antibody #5163293Untreated
Phospho-MYPT1 (Thr853) Antibody #4563HeLa, NIH/3T3Untreated
Phospho-Myt1 (Ser83) Antibody #4281HeLa, HT-29Nocadazole (50 ng/ml for 20 hr)
Phospho-MERIT40 (Ser29) Antibody #12110A-431Untreated
Phospho-p95/NBS1 (Ser343) Antibody #3001HeLaUV‡ (50 mJ/cm2), 2 hr recovery before lysing
Phospho-NDRG1 (Ser330) (D3A12) Rabbit mAb #11899A549, LNCaP, MCF7
Phospho-NDRG1 (Ser330) Antibody #3506JurkatCalyculin A (100 nM, 20 min)
Phospho-NDRG1 (Thr346) (D98G11) XP® Rabbit mAb #5482C2C12Untreated
Phospho-NDRG1 (Thr346) (D98G11) XP® Rabbit mAb (HRP Conjugate) #8947JurkatAkt Control Cell Extracts #9273
Phospho-NDRG1 (Thr346) Antibody #3217C2C12Insulin, 30 min
Phospho-NEDD4L (Ser342) (D16D6) Rabbit mAb #12146SK-MEL-28
Phospho-NEDD4L (Ser448) Antibody #8063SK-MEL-28Untreated
Phospho-NF-κB2 p100 (Ser866/870) Antibody #4810Transfected†NIK and p100 overexpression (cotransfected)
Phospho-NF-κB p105 (Ser933) (18E6) Rabbit mAb #4806HeLahTNF-α (20 ng/ml for 5 min)NF-κB Control Cell Extracts #9243
Phospho-NF-κB p65 (Ser468) Antibody #3039HeLahTNF-α (20 ng/ml for 5 min)NF-κB Control Cell Extracts #9243
Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb #3033HeLahTNF-α (20 ng/ml for 5 min)NF-κB Control Cell Extracts #9243
Phospho-NF-κB p65 (Ser536) (E1Z1T) Mouse mAb #13346differentiated THP-1TPA (80 nM, overnight), LPS (1 μg/ml for 15 min)
Phospho-NF-κB p65 (Ser536) (7F1) Mouse mAb #3036HeLahTNF-α (20 ng/ml for 5 min)NF-κB Control Cell Extracts #9243
Phospho-NF-κB p65 (Ser536) Antibody #3031HeLahTNF-α (20 ng/ml for 5 min)NF-κB Control Cell Extracts #9243
Methyl-NF-κB p65 (Lys310) Antibody #13188HT-1080, OVCAR8Untreated
Acetyl-NF-κB p65 (Lys310) (D2S3J) Rabbit mAb #12629Transfected†NF-κB p65 and CBP/p300 overexpression (cotransfected)
Acetyl-NF-κB p65 (Lys310) Antibody #3045Transfected†NF-κB p65 and CBP/p300 overexpression (cotransfected)
Phospho-NMDAR1 (Ser890) Antibody #3381Transfected†Transfect with construct expressing NMDAR1 and treat with TPA (100 nM for 5 min)
Phospho-NMDAR1 (Ser896) Antibody #3384Transfected†Transfect with construct expressing NMDAR1 and treat with TPA (100 nM for 5 min)
Phospho-NMDAR1 (Ser897) Antibody #3385Transfected†Transfect with construct expressing NMDAR1 and treat with Forskolin (30 μM for 20 min)
Phospho-NMDAR2A (Tyr1246) Antibody #4206Rat brainUntreated
Phospho-NMDAR2B (Tyr1070) Antibody #4209Rat brainUntreated
Phospho-NMDAR2B (Ser1284) Antibody #5355Fetal rat brain
Phospho-NMDAR2B (Tyr1472) Antibody #4208Rat brainUntreated
Cleaved Notch1 (Val1744) (D3B8) Rabbit mAb #4147MOLT-4, JurkatUntreated
Phospho-NPM (Ser4) (D19C1) XP® Rabbit mAb #3520HeLaNocadazole (50 ng/ml for 20 hr)
Phospho-NPM (Thr95) Antibody #3517HeLaNocadazole (50 ng/ml for 20 hr)
Phospho-NPM (Thr199) Antibody #3541HeLaNocadazole (50 ng/ml for 20 hr)
Phospho-NuMA (Ser395) Antibody #3429M059KUV‡ (100 mJ/cm2), 1 hr recovery
Phospho-NuMA (Thr2055) Antibody #14951HT-29Thymidine (2 mM for 16 hr), add fresh media and Nocadazole (100 ng/ml 24 hr), collect by mitotic shake off
Phospho-Numb (Ser276) (D5C2) Rabbit mAb #9878HeLaTPA (200 nM for 15-30 min)MEK1/2 HeLa Control Cell Extracts #9160
Phospho-Numb (Ser276) Antibody #4140RamosTPA (200 nM for 30 min)
Phospho-Nur77 (Ser351) (D22G5) Rabbit mAb #5095Jurkat cellsTPA #4174 (40 nM), A23187 (2 µM) both for 4 hr
Phospho-μ-Opioid Receptor (Ser375) Antibody #3451Transfected†Transfect 293 cells with mu-opioid receptor and treat with DAMGO (1 mM for 5 min)
Phospho-p130 Cas (Tyr165) Antibody #4015NIH/3T3Untreated
Phospho-p130 Cas (Tyr249) Antibody #4014NIH/3T3Untreated
Phospho-p130 Cas (Tyr410) Antibody #4011NIH/3T3Untreated
Phospho-p40phox (Thr154) Antibody #4311THP-1Serum-starve overnight, TPA (200 nM for 15 min)
Phospho-p53 (Ser6) Antibody #9285HT29, COS , 293, MCF7UV‡ (75 mJ/cm2), 0.5 to 2 hr recovery, 10 µM Etoposide (timing varies), 0.5 µM Doxorubicin (timing varies), 1 µM Camptothecin (timing varies), IR (10 Gy) wth recovery in fresh mediaSAPK/JNK Control Cell Extracts #9253
Phospho-p53 (Ser9) Antibody #9288HT29, COS , 293, MCF7UV‡ (75 mJ/cm2), 0.5 to 2 hr recovery, 10 µM Etoposide (timing varies), 0.5 µM Doxorubicin (timing varies), 1 µM Camptothecin (timing varies), IR (10 Gy) wth recovery in fresh mediaSAPK/JNK Control Cell Extracts #9253
Phospho-p53 (Ser15) (D4S1H) XP® Rabbit mAb (Rodent Specific) #12571L-929Etoposide (30 µM for 2 hr)
Phospho-p53 (Ser15) (16G8) Mouse mAb #9286HT29, COS , 293, MCF7UV‡ (75 mJ/cm2), 0.5 to 2 hr recovery, 10 µM Etoposide (timing varies), 0.5 µM Doxorubicin (timing varies), 1 µM Camptothecin (timing varies), IR (10 Gy) wth recovery in fresh mediaSAPK/JNK Control Cell Extracts #9253
Phospho-p53 (Ser15) Antibody #9284HT29, COS , 293, MCF7UV‡ (75 mJ/cm2), 0.5 to 2 hr recovery, 10 µM Etoposide (timing varies), 0.5 µM Doxorubicin (timing varies), 1 µM Camptothecin (timing varies), IR (10 Gy) wth recovery in fresh mediaSAPK/JNK Control Cell Extracts #9253
Phospho-p53 (Thr18) Antibody #2529HT29, COS , 293, MCF7Doxorubicin (0.5 µM for 24 hr)
Phospho-p53 (Ser20) Antibody #9287HT29, COS , 293, MCF7UV‡ (75 mJ/cm2), 0.5 to 2 hr recovery, 10 µM Etoposide (timing varies), 0.5 µM Doxorubicin (timing varies), 1 µM Camptothecin (timing varies), IR (10 Gy) wth recovery in fresh mediaSAPK/JNK Control Cell Extracts #9253
Phospho-p53 (Ser33) Antibody #2526HT29, COS , 293, MCF7Nocadazole (50 ng/ml for 24–48 hr)
Phospho-p53 (Ser37) Antibody #9289HT29, COS , 293, MCF7UV‡ (75 mJ/cm2), 0.5 to 2 hr recovery, 10 µM Etoposide (timing varies), 0.5 µM Doxorubicin (timing varies), 1 µM Camptothecin (timing varies), IR (10 Gy) wth recovery in fresh mediaSAPK/JNK Control Cell Extracts #9253
Phospho-p53 (Ser46) Antibody #2521HT29, COS , 293, MCF7UV‡ (75 mJ/cm2), 2 hr recovery
Phospho-p53 (Thr81) Antibody #2676HeLaNocadazole (50 ng/ml for 24–48 hr)
Phospho-p53 (Ser315) Antibody #2528HT29, COS , 293, MCF7Nocadazole (50 ng/ml for 24–48 hr)
Phospho-p53 (Ser392) Antibody #9281HT29, COS , 293, MCF7Hydroxyurea (20 mM for 30 hr)
Acetyl-p53 (Lys382) Antibody #2525HT29, COS , 293, MCF7Doxorubicin (0.5 µM for 24 hr); 400 nM TSA can be used in conjunction to stabilize activated protein
Acetyl-p53 (Lys379) Antibody #2570HT29, COS , 293, MCF7Doxorubicin (0.5 µM for 24 hr); 400 nM TSA can be used in conjunction to stabilize activated protein
Phospho-p57 Kip2 (Thr310) Antibody #2558HeLaDexamethasone (50 nM for 16 hr)
Phospho-p63 (Ser160/162) Antibody #4981ME-180Nocadazole (50 ng/ml for 24 hr)
Phospho-p70 S6 Kinase (Ser371) Antibody #9208MCF7Serum-starve overnight, +hIGF-1 (100 ng/ml for 10 min)p70 S6 Kinase MCF7 Control Cell Extracts #34499
Phospho-Drosophila p70 S6 Kinase (Thr398) Antibody #9209MCF7Serum-starve overnight, +hIGF-1 (100 ng/ml for 10 min)p70 S6 Kinase MCF7 Control Cell Extracts #34499
Phospho-p70 S6 Kinase (Thr389) (108D2) Rabbit mAb #9234MCF7Serum-starve overnight, +hIGF-1 (100 ng/ml for 10 min)p70 S6 Kinase MCF7 Control Cell Extracts #34499
Phospho-p70 S6 Kinase (Thr389) (1A5) Mouse mAb #9206MCF7Serum-starve overnight, +hIGF-1 (100 ng/ml for 10 min)p70 S6 Kinase MCF7 Control Cell Extracts #34499
Phospho-p70 S6 Kinase (Thr389) Antibody #9205MCF7Serum-starve overnight, +hIGF-1 (100 ng/ml for 10 min)p70 S6 Kinase MCF7 Control Cell Extracts #34499
Phospho-p70 S6 Kinase (Thr421/Ser424) Antibody #9204MCF7Serum-starve overnight, +hIGF-1 (100 ng/ml for 10 min)p70 S6 Kinase MCF7 Control Cell Extracts #34499
Phospho-p73 (Tyr99) Antibody #4665HT-1376Pervanadate (1 mM for 20 min)
Asymmetric-Methyl-PABP1 (Arg455/Arg460) (C60A10) Rabbit mAb #3505HeLa, C6, COSUntreated
Phospho-PAK1 (Ser199/204)/PAK2 (Ser192/197) Antibody #2605Guinea pig neutrophilsfMLP (1 µM for 30 sec)
Phospho-PAK1 (Thr423)/PAK2 (Thr402) Antibody #2601Guinea pig neutrophilsfMLP (1 µM for 30 sec)
Phospho-PAK1 (Ser144)/PAK2 (Ser141) Antibody #2606Guinea pig neutrophilsfMLP (1 µM for 30 sec)
Phospho-PAK2 (Ser20) Antibody #2607Guinea pig neutrophilsfMLP (1 µM for 30 sec)
Phospho-PAK4 (Ser474)/PAK5 (Ser602)/PAK6 (Ser560) Antibody #3241Guinea pig neutrophilsfMLP (1 µM for 30 sec)
Phospho-PAR-4 (Thr163) Antibody #2329HeLaCalyculin A (100 nM for 5 min)
Cleaved PARP (Asp214) (D64E10) XP® Rabbit mAb #5625HeLaSerum-starve overnight, Staurosporine (1 µM for 3 hr)
Cleaved PARP (Asp214) (19F4) Mouse mAb (Human Specific) #9546HeLaSerum-starve overnight, Staurosporine (1 µM for 3 hr)Jurkat Apoptosis Cell Lysates (etoposide) #2043
Cleaved PARP (Asp214) (7C9) Mouse mAb (Mouse Specific) #9548L-929Serum-starve overnight, Staurosporine (1 µM for 3 hr)
Cleaved PARP (Asp214) Antibody (Mouse Specific) #9544L-929Serum-starve overnight, Staurosporine (1 µM for 3 hr)
Cleaved PARP (Asp214) Antibody (Rat Specific) #9545C6Serum-starve overnight, Staurosporine (1 µM for 3 hr)
Cleaved PARP (Asp214) Antibody (Human Specific) #9541HeLaSerum-starve overnight, Staurosporine (1 µM for 3 hr)Jurkat Apoptosis Cell Lysates (etoposide) #2043
Phospho-Paxillin (Tyr118) Antibody #2541C2C12, NIH/3T3, A-431, C6Untreated
Phospho-PBK/TOPK (Thr9) Antibody #4941HT-29Nocadazole (50 ng/ml for 20 hr)
Phospho-PDGF Receptor α (Tyr849)/PDGF Receptor β (Tyr857) (C43E9) Rabbit mAb #3170NIH/3T3Serum-starve overnight, PDGF-β (100 ng/ml for 5 min)
Phospho-PDGF Receptor α (Tyr754) (23B2) Rabbit mAb #2992NIH/3T3Serum-starve overnight, PDGF-β (100 ng/ml for 5 min)
Phospho-PDGF Receptor α (Tyr762) (D9B1N) Rabbit mAb #24188NCI-H1703hPDGF-AA (100 ng/ml for 10 min)
Phospho-PDGF Receptor α (Tyr762) (D9B1N) Rabbit mAb #24188NCI-H1703Human Platelet-Derived Growth Factor AA (hPDGF-AA) #8913 (100 ng/ml for 10 min)
Phospho-PDGF Receptor α (Tyr1018) Antibody #4547NIH/3T3Serum-starve overnight, PDGF-β (100 ng/ml for 5 min)
Phospho-PDGF Receptor β (Tyr740) (32A9) Rabbit mAb #3168NIH/3T3Serum-starve overnight, PDGF-β (100 ng/ml for 5 min)
Phospho-PDGF Receptor β (Tyr751) (C63G6) Rabbit mAb #4549NIH/3T3Serum-starve overnight, PDGF-β (100 ng/ml for 5 min)
Phospho-PDGF Receptor β (Tyr751) (88H8) Mouse mAb #3166NIH/3T3Serum-starve overnight, PDGF-β (100 ng/ml for 5 min)
Phospho-PDGF Receptor β (Tyr751) Antibody #3161NIH/3T3Serum-starve overnight, PDGF-β (100 ng/ml for 5 min)
Phospho-PDGF Receptor β (Tyr771) (76D6) Rabbit mAb #3173NIH/3T3Serum-starve overnight, PDGF-β (100 ng/ml for 5 min)
Phospho-PDGF Receptor β (Tyr1009) (42F9) Rabbit mAb #3124NIH/3T3Serum-starve overnight, PDGF-β (100 ng/ml for 5 min)
Phospho-PDGF Receptor β (Tyr1021) (6F10) Rabbit mAb #2227NIH/3T3Serum-starve overnight, PDGF-β (100 ng/ml for 5 min)
Phospho-PDK1 (Ser241) (C49H2) Rabbit mAb #3438JurkatUntreated
Phospho-PDK1 (Ser241) Antibody #3061HeLaUntreated
Phospho-PEA-15 (Ser104) Antibody #2776C6TPA (200 nM for 1 hr)
Phospho-PERK (Thr980) (16F8) Rabbit mAb #3179C6Thapsigargin (1 μM for 20 min)
Phospho-PFKFB2 (Ser483) (D4R1W) Rabbit mAb #13064HeLaSerum-starve overnight; IGF-1 (100 ng/ml for 5 min)
Phospho-PFKFB2 (Ser483) (D4R1W) Rabbit mAb #13064HeLaIGF-1 (100 ng/ml for 5 min)
Phospho-(Ser/Thr) Phe Antibody #9631JurkatCalyculin A (100 nM for 30 min)Akt Control Cell Extracts #9273
Phospho-PI3 Kinase p85 (Tyr458)/p55 (Tyr199) Antibody #4228NIH/3T3/SrcUntreated
Phospho-PI3 Kinase Class III (Ser249) Antibody #13857Transfected
Phospho-PKA C (Thr197) (D45D3) Rabbit mAb #5661NIH/3T3Untreated
Phospho-PKA C (Thr197) Antibody #4781NIH-3T3
Phospho-PKCα/β II (Thr638/641) Antibody #9375NIH/3T3Serum-starve overnight, PDGF (100 ng/ml for 30 min)
Phospho-PKCδ (Ser359) (D2X1P) Rabbit mAb #14787OVCAR8, HeLa
Phospho-PKCdelta (Tyr311) Antibody #2055C6Serum-starve overnight, TPA (200 nM for 30 min)
Phospho-PKCδ (Thr505) Antibody #9374NIH/3T3Serum-starve overnight, PDGF (100 ng/ml for 30 min)
Phospho-PKCδ/θ (Ser643/676) Antibody #9376NIH/3T3Serum-starve overnight, PDGF (100 ng/ml for 30 min)
Phospho-PKCζ/λ (Thr410/403) Antibody #9378293Serum-starve overnight, hEGF (100 ng/ml for 10 min)
Phospho-PKC (pan) (gamma Thr514) (D6Y3D) Rabbit mAb #38938OVCAR8, Ramos
Phospho-PKC (pan) (gamma Thr514) Antibody #9379NIH/3T3Serum-starve overnight, PDGF (100 ng/ml for 30 min)
Phospho-PKC (pan) (βII Ser660) Antibody #9371NIH/3T3Serum-starve overnight, PDGF (100 ng/ml for 30 min)
Phospho-PKC (pan) (zeta Thr410) (190D10) Rabbit mAb #2060COSSerum-starve overnight, TPA (200 nM for 30 min)
Phospho-PKCθ (Thr538) Antibody #9377HeLaSerum-starve overnight, TPA (200 nM for 30 min)
Phospho-(Ser/Thr) PKD Substrate Antibody #4381HeLaTPA (200 nM for 30 min)MEK1/2 HeLa Control Cell Extracts #9160
Phospho-PKD/PKCμ (Ser744/748) Antibody #2054NIH/3T3Serum-starve overnight, PDGF (100 ng/ml for 30 min)
Phospho-PKD/PKCμ (Ser916) Antibody #2051NIH/3T3Serum-starve overnight, PDGF (100 ng/ml for 30 min)
Phospho-PKM2 (Tyr105) Antibody #3827A549Untreated
Phospho-cPLA2 (Ser505) Antibody #2831HeLaSerum-starve overnight, TPA (200 nM for 15 min)MEK1/2 HeLa Control Cell Extracts #9160
Phospho-Phospholamban (Ser16/Thr17) Antibody #8496spontaneous hypertensive (SHR) rat heart
Phospho-PLCbeta3 (Ser537) Antibody #2481HeLaSerum-starve overnight, TPA (200 nM for 15 min)MEK1/2 HeLa Control Cell Extracts #9160
Phospho-PLCbeta3 (Ser1105) Antibody #2484HeLaSerum-starve overnight, TPA (200 nM for 15 min)MEK1/2 HeLa Control Cell Extracts #9160
Phospho-PLCγ1 (Tyr783) (D6M9S) Rabbit mAb #14008A-431, NIH-3T3
Phospho-PLCγ1 (Tyr783) Antibody #2821NIH/3T3Serum-starve overnight, PDGF (50 ng/ml for 30 min)
Phospho-PLCγ1 (Ser1248) (D25A9) Rabbit mAb #8713NIH/3T3hPDGF-BB (50 ng/ml for 15 min)
Phospho-PLCγ1 (Ser1248) Antibody #4510COSTPA (200 nM for 20 min)
Phospho-PLCγ2 (Tyr759) Antibody #3874Ramos, RajiIgM (12 µg/ml for 2 min)
Phospho-PLCγ2 (Tyr1217) Antibody #3871Ramos, RajiIgM (12 µg/ml for 2 min)
Phospho-PLD1 (Thr147) Antibody #3831HeLaSerum-starve overnight, TPA (200 nM for 15 min)MEK1/2 HeLa Control Cell Extracts #9160
Phospho-PLD1 (Ser561) Antibody #3834HeLaSerum-starve overnight, TPA (200 nM for 15 min)MEK1/2 HeLa Control Cell Extracts #9160
Phospho-PLK1 (Thr210) (D5H7) Rabbit mAb #9062HeLa, HT-29Thymidine (2 mM for 16 hr), add fresh media and Nocadazole (100 ng/ml for 24 hr), collect by mitotic shake off
Phospho-PLK1 (Thr210) Antibody #5472HeLa, HT-29Thymidine (2 mM for 16 hr), add fresh media and Nocadazole (100 ng/ml for 24 hr), collect by mitotic shake off
Phospho-PNK1 (Ser114/Thr118) Antibody #3522HeLaUV‡ (100 mJ/cm2), 1 hr recovery
Phospho-Rpb1 CTD (Ser2/Ser5) (D1G3K) Rabbit mAb #13546C2C12, H-4-II-EUntreated
Phospho-Rpb1 CTD (Ser2) (E1Z3G) Rabbit mAb #13499C2C12, H-4-II-EUntreated
Phospho-Rpb1 CTD (Ser2/5) Antibody #4735MCF7Doxorubicin (0.5 µM for 36 hr)
Phospho-Rpb1 CTD (Thr4) Antibody #14934C2C12, H-4-II-EUntreated
Phospho-Rpb1 CTD (Ser5) (D9N5I) Rabbit mAb #13523C2C12, H-4-II-EUntreated
Phospho-Rpb1 CTD (Ser7) (E2B6W) Rabbit mAb #13780C2C12, H-4-II-EUntreated
Phospho-PP1α (Thr320) Antibody #2581HeLa, COS, NIH/3T3, C6Untreated
Phospho-PPIG (Ser376) Antibody #3804JurkatUntreated
Phospho-PRAS40 (Ser183) Antibody #5936MCF7Serum-starve overnight, +hIGF-1 (100 ng/ml for 10 min)p70 S6 Kinase MCF7 Control Cell Extracts #34499
Phospho-PRAS40 (Thr246) (D4D2) XP® Rabbit mAb #13175MCF7 -AA/+Insulin (100 nM, 30 min)4E-BP1 Control Cell Extracts #53167
Phospho-PRAS40 (Thr246) (C77D7) Rabbit mAb #2997MCF7Serum-starve overnight, +hIGF-1 (100 ng/ml for 10 min)p70 S6 Kinase MCF7 Control Cell Extracts #34499
Phospho-PRAS40 (Thr246) Antibody #2640MCF7Serum-starve overnight, +hIGF-1 (100 ng/ml for 10 min)p70 S6 Kinase MCF7 Control Cell Extracts #34499
Phospho-Prdx1 (Tyr194) (D1T9C) Rabbit mAb #14041A431EGF (50 ng/ml for 15 min)
Phospho-PRK1 (Thr774)/PRK2 (Thr816) Antibody #2611JurkatUntreated or Calyculin A (100 nM for 30 min)Akt Control Cell Extracts #9273
Phospho-Progesterone Receptor (Ser190) Antibody #3171T-47DSerum-starve overnight, promegestone (100 nM for 1 hr)
Phospho-Progesterone Receptor (Ser294) Antibody #13736T-47DPromegestone (100 nM for 1 hr)
Phospho-Progesterone Receptor (Ser345) Antibody #12783T-47DPromegestone (100 nM for 1 hr)
Phospho-Progesterone Receptor (Ser345) Antibody #12783T-47DPromegestone (R5020-treated, 100 nM for 1 hr)
Phospho-PSD93 (Tyr340) Antibody #2930Rat brainUntreated
Phospho-PSD95 (Tyr236/Tyr240) Antibody #3919Rat brainUntreated
Phospho-PSD95 (Ser295) (A8F8Z) Rabbit mAb #45737mouse brain, rat brainUntreated
Phospho-PTEN (Ser380/Thr382/383) (44A7) Rabbit mAb #9549HeLaUntreated
Phospho-PTEN (Ser380/Thr382/383) Antibody #9554HeLaUntreated
Phospho-PTEN (Ser380) Antibody #9551HeLaUntreated
Phospho-Thr-Pro-Pro Motif [pTPP] (D61C3) Rabbit mAb #5757HCT 116G0 cells (-): serum starved and contact inhibited (36 hr). Mitotic cells (+) thymidine (2 mM for 18 hr) followed by Nocadazole (50 ng/ml for 20 hr)
Phospho-(Ser) CDKs Substrate (P-S2-100) Rabbit mAb #9477HeLaNocadazole (1 μg/ml for 12 hr)
Phospho-Thr-Pro-Arg Motif [pTPR] MultiMab™ Rabbit mAb mix #8142Jurkatuntreated (-) or treated with Calyculin A (10 ng/ml for 20 min; +)AKT control cell extracts #9273
Phospho-(Ser) PKC Substrate (P-S3-101) Rabbit mAb #6967HeLaTPA (200 nM for 30 min)MEK1/2 HeLa Control Cell Extracts #9160
Phospho-MAPK/CDK Substrates (PXS*P or S*PXR/K) (34B2) Rabbit mAb #2325HeLaNocadazole (1 μg/ml for 12 hr)
Phospho-(Ser) PKC Substrate Antibody #2261HeLaTPA (200 nM for 30 min)MEK1/2 HeLa Control Cell Extracts #9160
Phospho-Threonine-X-Arginine Antibody #2351JurkatCalyculin A (100 nM for 30 min)Akt Control Cell Extracts #9273
Phospho-PTPα (Tyr789) Antibody #4481293, COS, C6, C2C12Untreated
Phospho-Pyk2 (Tyr402) Antibody #3291JurkatSerum-starve overnight, anti-CD3 plus anti-CD28 (both 1 µg/ml for 10 min)
Phospho-PZR (Tyr241) (D6F9) Rabbit mAb #8131BAECH2O2 (0.3 mM for 30 min)
Phospho-PZR (Tyr263) (D6A5) Rabbit mAb #8088BAECH2O2 (0.3 mM for 30 min)
Phospho-PZR (Tyr263) Antibody #5543BAECH2O2 (0.3 mM for 30 min)
Phospho-Rac1/cdc42 (Ser71) Antibody #2461A-431Serum-starve overnight, hEGF (100 ng/ml for 10 min)
Phospho-Rad17 (Ser635) (D6K9P) Rabbit mAb #13404A549UV‡ (100 mJ/cm2), 2 hr recovery before lysing
Phospho-Rad17 (Ser645) (D5H5) Rabbit mAb #6981MRC-5UV‡ (100 mJ/cm2), 2 hr recovery
Phospho-Rad18 (Ser403) (D2T6W) Rabbit mAb #14978HeLaUV‡ (100 mJ/cm2), 2 hr recovery before lysing
Phospho-Rad18 (Ser403) Antibody #8393HeLaUV‡ (100 mJ/cm2), 2 hr recovery
Phospho-Rad50 (Ser635) Antibody #14223293UV‡ (100 mJ/cm2), 2 hr recovery before lysing
Phospho-A-Raf (Ser299) Antibody #4431HeLaSerum-starve overnight, TPA (200 nM for 15 min)MEK1/2 HeLa Control Cell Extracts #9160
Phospho-B-Raf (Ser445) Antibody #2696HeLaSerum-starve overnight, TPA (200 nM for 30 min)MEK1/2 HeLa Control Cell Extracts #9160
Phospho-c-Raf (Ser259) Antibody #9421HeLaSerum-starve overnight, TPA (200 nM for 15 min)MEK1/2 HeLa Control Cell Extracts #9160
Phospho-c-Raf (Ser289/296/301) Antibody #9431HeLaSerum-starve overnight, TPA (200 nM for 30 min)MEK1/2 HeLa Control Cell Extracts #9160
Phospho-c-Raf (Ser296) Antibody #9423HeLaSerum-starve overnight, TPA (200 nM for 15 min)MEK1/2 HeLa Control Cell Extracts #9160
Phospho-c-Raf (Ser338) (56A6) Rabbit mAb #9427HeLaSerum-starve overnight, TPA (200 nM for 15 min)MEK1/2 HeLa Control Cell Extracts #9160
Phospho-RanBP3 (Ser58) Antibody #9380HeLaSerum-starve overnight, hEGF (100 ng/ml for 10 min)
Phospho-Raptor (Ser792) Antibody #2083293Oligomycin (0.5 µM for 30 min)
Phospho-Ras-GRF1 (Ser916) Antibody #3321Transfected†Transfect with construct overexpressing Ras-GRF1, Forskolin (30 µM for 5 min)
Phospho-Rb (Ser608) (D10F2) Rabbit mAb #8147HT-29Nocadazole (100 ng/ml for 18 hr); treatment induces slight phospho-shiftRb Control Proteins #9303
Phospho-Rb (Ser608) Antibody #2181HT-29Nocadazole (100 ng/ml for 18 hr); treatment induces slight phospho-shiftRb Control Proteins #9303
Phospho-Rb (Ser780) (C84F6) Rabbit mAb #3590HT-29Nocadazole (100 ng/ml for 18 hr); treatment induces slight phospho-shiftRb Control Proteins #9303
Phospho-Rb (Ser780) (D59B7) Rabbit mAb #8180HT-29Nocadazole (100 ng/ml for 18 hr); treatment induces slight phospho-shiftRb Control Proteins #9303
Phospho-Rb (Ser780) Antibody #9307HT-29Nocadazole (100 ng/ml for 18 hr); treatment induces slight phospho-shiftRb Control Proteins #9303
Phospho-Rb (Ser795) Antibody #9301HT-29Nocadazole (100 ng/ml for 18 hr); treatment induces slight phospho-shiftRb Control Proteins #9303
Phospho-Rb (Ser807/811) (D20B12) XP® Rabbit mAb #8516MCF7UntreatedRb Control Proteins #9303
Phospho-Rb (Ser807/811) Antibody #9308MCF7UntreatedRb Control Proteins #9303
Phospho-RCC1 (Ser11) (D18B5) Rabbit mAb #5500HeLaThymidine (2 mM 16 hr), add fresh media and Nocadazole (100 ng/ml 24 hr), collect by mitotic shake off
Phospho-RCC1 (Ser11) Antibody #4210HT-29Thymidine (2 mM for 16 hr) followed by Nocadazole (100 ng/ml for 24 hr)
Phospho-RelB (Ser552) (D41B9) XP® Rabbit mAb #5025Jurkat, RajiTPA (200 nM for 30 min)
Phospho-RelB (Ser552) Antibody #4999Jurkat, RajiTPA (200 nM for 30 min)
Phospho-REPS1 (Ser709) (D8C1) Rabbit mAb #12005JurkatAkt Control Cell Extracts #9273
Phospho-Ret (Tyr905) Antibody #3221TTUntreated
Phospho-Rev-erbα (Ser55/59) Antibody #2129Transfected†Transfect with a construct overexpressing Rev-Erbα
Phospho-Rictor (Thr1135) (D30A3) Rabbit mAb #3806MCF7Serum-starve overnight, +hIGF-1 (100 ng/ml for 10 min)p70 S6 Kinase MCF7 Control Cell Extracts #34499
Phospho-RIP2 (Ser176) (E1I9J) Rabbit mAb #14397THP-1TPA (80 nM for 24 hr) plus LPS (1 µg/ml for 15 min)
Phospho-RIP (Ser166) (D1L3S) Rabbit mAb #65746HT-29Pre-treat Z-VAD (20 μM for 30 min) followed by addition of CHX (10 μg/ml) and hTNF-α (20 ng/ml) overnight
Phospho-ROS1 (Tyr2274) Antibody #3078HCC78HCC78 cells express a SLC3482-Ros fusion protein
Phospho-p90RSK (Thr359) (D1E9) Rabbit mAb #8753HeLaSerum-starve overnight, TPA (200 nM for 15 min)MEK 1/2 Control Cell Extracts #9160
Phospho-p90RSK (Thr573) Antibody #9346HeLaTPA (200 nM for 30 min)MEK1/2 HeLa Control Cell Extracts #9160
Phospho-RSK2 (Ser227) (D53A11) Rabbit mAb #3556HeLaTPA (200 nM for 30 min)MEK1/2 HeLa Control Cell Extracts #9160
Phospho-p90RSK (Ser380) (D5D8) Rabbit mAb #12032HeLaSerum-starve overnight, TPA (200 nM for 15 min)MEK 1/2 Control Cell Extracts #9160
Phospho-p90RSK (Ser380) (9D9) Rabbit mAb #9335HeLaTPA (200 nM for 30 min)MEK1/2 HeLa Control Cell Extracts #9160
Phospho-p90RSK (Ser380) (D3H11) Rabbit mAb #11989HeLaSerum-starve overnight, TPA (200 nM for 15 min)MEK 1/2 Control Cell Extracts #9160
Phospho-RSK3 (Thr356/Ser360) Antibody #9348HeLaSerum-starve overnight, TPA (200 nM for 15 min)MEK1/2 HeLa Control Cell Extracts #9160
Phospho-p90RSK (Ser380) Antibody #9341HeLaTPA (200 nM for 30 min)MEK1/2 HeLa Control Cell Extracts #9160
Phospho-p90RSK (Thr359/Ser363) Antibody #9344HeLaTPA (200 nM for 30 min)MEK1/2 HeLa Control Cell Extracts #9160
Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb #4858MCF7Serum-starve overnight, +hIGF-1 (100 ng/ml for 10 min)p70 S6 Kinase MCF7 Control Cell Extracts #34499
Phospho-S6 Ribosomal Protein (Ser235/236) (91B2) Rabbit mAb #4857MCF7Serum-starve overnight, +hIGF-1 (100 ng/ml for 10 min)p70 S6 Kinase MCF7 Control Cell Extracts #34499
Phospho-S6 Ribosomal Protein (Ser235/236) (2F9) Rabbit mAb #4856MCF7Serum-starve overnight, +hIGF-1 (100 ng/ml for 10 min)p70 S6 Kinase MCF7 Control Cell Extracts #34499
Phospho-S6 Ribosomal Protein (Ser235/236) Antibody #2211MCF7Serum-starve overnight, +hIGF-1 (100 ng/ml for 10 min)p70 S6 Kinase MCF7 Control Cell Extracts #34499
Phospho-S6 Ribosomal Protein (Ser240/244) (D68F8) XP® Rabbit mAb #5364MCF7Serum-starve overnight, +hIGF-1 (100 ng/ml for 10 min)p70 S6 Kinase MCF7 Control Cell Extracts #34499
Phospho-S6 Ribosomal Protein (Ser240/244) Antibody #2215MCF7Serum-starve overnight, +hIGF-1 (100 ng/ml for 10 min)p70 S6 Kinase MCF7 Control Cell Extracts #34499
Phospho-SAMHD1 (Thr592) Antibody #15038THP-1Untreated
Phospho-GKAP (Ser346) Antibody #13638mouse brain, rat brainUntreated
Phospho-SAPK/JNK (Thr183/Tyr185) (81E11) Rabbit mAb #4668293UV‡ (40 mJ/cm2), 30 min recovery timeSAPK/JNK Control Cell Extracts #9253
Phospho-SAPK/JNK (Thr183/Tyr185) (98F2) Rabbit mAb #4671293UV‡ (40 mJ/cm2), 30 min recovery timeSAPK/JNK Control Cell Extracts #9253
Phospho-SAPK/JNK (Thr183/Tyr185) (G9) Mouse mAb #9255293UV‡ (40 mJ/cm2), 30 min recoverySAPK/JNK Control Cell Extracts #9253
Phospho-SAPK/JNK (Thr183/Tyr185) Antibody #9251293UV‡ (40 mJ/cm2), 30 min recoverySAPK/JNK Control Cell Extracts #9253
Phospho-SATB1 (Ser47) Antibody #4028JurkatUntreated
Phospho-Scribble (Ser1220) (D8A2) Rabbit mAb #12316293T, PANC-1, HCT 116
Phospho-SEK1/MKK4 (Ser80) Antibody #9155NIH/3T3PDGF (100 ng/ml for 5 min)
Phospho-SEK1/MKK4 (Ser257) (C36C11) Rabbit mAb #4514HeLaUV‡ (50 mJ/cm2), 30 min recovery
Phospho-SEK1/MKK4 (Ser257/Thr261) Antibody #9156HeLaUV‡ (50 mJ/cm2), 30 min recovery
Phospho-SEK1/MKK4 (Thr261) Antibody #9151HeLaUV‡ (50 mJ/cm2), 30 min recovery
Phospho-Semaphorin 4B (Ser825) Antibody #5622MKN-45Untreated
Phospho-SF3B1 (Thr313) (D8D8V) Rabbit mAb #25009HeLa, 3T3, Cos-7Untreated
Phospho-SGK1 (Ser78) (D36D11) Rabbit mAb #5599SK-MEL-28H2O2 (4 mM for 15 min)
Phospho-SGK (Ser78) Antibody #3271Transfected†Transfect with construct overexpressing SGK
Phospho-SGK3 (Thr320) (D30E6) Rabbit mAb #5642MCF7H2O2 (4 mM for 15 min)
Phospho-SGTA (Ser305) (D23E10) Rabbit mAb #8664HeLa
Phospho-Shc (Tyr239/240) Antibody #2434Hep G2Serum-starve overnight, hEGF (100 ng/ml for 5 min)
Phospho-Shc (Tyr317) Antibody #2431Hep G2Serum-starve overnight, hEGF (100 ng/ml for 5 min)
Phospho-SHIP1 (Tyr1020) Antibody #3941RamosAnti-human IgM (12 µg/ml for 2 min)
Phospho-SHIP2 (Tyr986/987) Antibody #2008K-562Untreated
Phospho-SHIP2 (Tyr1135) (D33C6) XP® Rabbit mAb #5445K-562Untreated
Phospho-SHIP2 (Tyr1135) Antibody #2007K-562Untreated
Phospho-SHP-1 (Tyr564) (D11G5) Rabbit mAb #8849JurkatAkt Control Cell Extracts #9273
Phospho-SHP-2 (Tyr542) Antibody #3751NIH/3T3Serum-starve overnight, PDGF (100 ng/ml for 5 min)
Phospho-SHP-2 (Tyr580) (D66F10) Rabbit mAb #5431C6 cellsSerum-starved overnight and treated with PDGF-BB (50 ng/ml for 5 min)
Phospho-SHP-2 (Tyr580) (D66F10) Rabbit mAb (PE Conjugate) #13328NIH-3T3Serum-starved overnight and treated with PDGF-BB (50 ng/ml for 5 min)
Phospho-SHP-2 (Tyr580) Antibody #3703NIH/3T3Serum-starve overnight, PDGF-β (100 ng/ml for 5 min)
Phospho-SirT1 (Ser27) Antibody #2327293Untreated
Phospho-SirT1 (Ser47) Antibody #2314293Untreated
Phospho-Skp2 (Ser64) Antibody #14865293T, HeLa
Phospho-SLP-76 (Tyr145) Antibody #14770JurkatAnti-CD3 (10 µg/ml for 2 min)
Phospho-SLP-76 (Ser376) (D9D6E) Rabbit mAb #14745JurkatAnti-CD3 (10 µg/ml for 2 min)
Phospho-Smad1 (Ser206) (D40B7) Rabbit mAb #5753HeLaSerum-starve overnight, UV‡ (50 mJ/cm2), 1 hr recovery
Phospho-Smad1 (Ser206) Antibody #9553HeLaSerum-starve overnight, UV‡ (50 mJ/cm2), 1 hr recovery
Phospho-Smad1/5 (Ser463/465) (41D10) Rabbit mAb #9516HeLa, HT-1080, C2C12, C6Serum-starve overnight, BMP (50 ng/ml for 30 min)
Phospho-Smad1 (Ser463/465)/ Smad5 (Ser463/465)/ Smad9 (Ser465/467) (D5B10) Rabbit mAb #13820HelaBMP2 (50mg, 30min)
Phospho-Smad1 (Ser463/465)/ Smad5 (Ser463/465)/ Smad9 (Ser465/467) (D5B10) Rabbit mAb (ChIP formulated) #11971MCF7BMP2 (50mg, 60min)
Phospho-Smad2 (Ser245/250/255) Antibody #3104HeLaSerum-starve overnight, TPA (200 nM for 30 min)
Phospho-Smad2 (Ser465/467) (138D4) Rabbit mAb #3108HeLa, HT-1080, C2C12, KNRKSerum-starve overnight, TGF-β (10 ng/ml for 30 min)Smad2/3 Control Cell Extracts #12052
Phospho-Smad2 (Ser465/467) Antibody #3101HeLa, HT-1080, C2C12, KNRKSerum-starve overnight, TGF-β (10 ng/ml for 30 min)Smad2/3 Control Cell Extracts #12052
Phospho-Smad2 (Ser465/467)/Smad3 (Ser423/425) (D27F4) Rabbit mAb #8828HT-1080, C2C12, KNRKUntreated or Treated with TGF-β (10 ng/ml for 30 min)Smad2/3 Control Cell Extracts #12052
Phospho-Smad3 (Ser423/425) (C25A9) Rabbit mAb #9520HT-1080, C2C12, KNRKSerum-starve overnight, TGF-β (10 ng/ml for 30 min)Smad2/3 Control Cell Extracts #12052
Phospho-SMC1 (Ser360) Antibody #4029293UV‡ (100 mJ/cm2), 4 hr recovery before lysing
Phospho-SMC1 (Ser957) (5D11G5) Mouse mAb #4805RAW 264.7, 293UV‡ (100 mJ/cm2), 1 hr recovery before lysingSAPK/JNK Control Cell Extracts #9253
Phospho-SMC1 (Ser957) Antibody #4801RAW 264.7, 293UV‡ (100 mJ/cm2), 1 hr recovery before lysingSAPK/JNK Control Cell Extracts #9253
Phospho-Sox2 (Ser250/Ser251) Antibody #77627NCCITUntreated
Phospho-SQSTM1/p62 (Thr269/Ser272) Antibody #13121ACHN Untreated
Phospho-SQSTM1/p62 (Ser349) Antibody #95697293TSodium arsenite (15 µM overnight)
Phospho-Src (Ser17) (D7F2Q) Rabbit mAb #12432JurkatUntreated
Phospho-Src (Ser17) Antibody #5473MCF7Untreated
Phospho-Src (Tyr527) Antibody #2105COLO-201Untreated
Phospho-Src Family (Tyr416) (D49G4) Rabbit mAb #6943COLO-201Untreated
Phospho-Src Family (Tyr416) Antibody #2101COLO-201Untreated
Phospho-SRC-3 (Thr24) Antibody #2979MCF7Untreated
Phospho-SREBP-1c (Ser372) Antibody #9874Transfectedserum-starved cells treated with resveratrol (50 µM for 1 hr)
Phospho-SRF (Ser103) Antibody #4261HeLaSerum-starve overnight, TPA (200 nM for 15 min)MEK1/2 HeLa Control Cell Extracts #9160
Phospho-SSH3 (Ser37) Antibody #13873293T, ZR-75
Phospho-Stat1 (Tyr701) (58D6) Rabbit mAb #9167HeLaSerum-starve overnight, IFN-α (100 ng/ml for 5 min)Stat1 Control Cell Extracts #9173
Phospho-Stat1 (Tyr701) (D4A7) Rabbit mAb #7649HeLaSerum-starve overnight, IFN-α (100 ng/ml for 5 min)Stat1 Control Cell Extracts #9173
Phospho-Stat1 (Ser727) (D3B7) Rabbit mAb #8826HeLaUV‡ (50 mJ/cm2), 2 hr recovery
Phospho-Stat1 (Ser727) Antibody #9177HeLaUV‡ (50 mJ/cm2), 2 hr recovery
Phospho-Stat2 (Tyr690) (D3P2P) Rabbit mAb #88410U266, A-431hIFN-α1 (10 ng for 5min)
Phospho-Stat2 (Tyr690) Antibody #4441HeLaSerum-starve overnight, IFN-α (100 ng/ml for 15 min)
Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb #9145HeLaSerum-starve overnight, IFN-α (100 ng/ml for 5 min)Stat3 Control Cell Extracts #9133
Phospho-Stat3 (Tyr705) (3E2) Mouse mAb #9138HeLaSerum-starve overnight, IFN-α (100 ng/ml for 5 min)Stat3 Control Cell Extracts #9133
Phospho-Stat3 (Tyr705) (M9C6) Mouse mAb #4113HeLaSerum-starve overnight, IFN-α (100 ng/ml for 5 min)Stat3 Control Cell Extracts #9133
Phospho-Stat3 (Tyr705) Antibody #9131HeLaSerum-starve overnight, IFN-α (100 ng/ml for 5 min)Stat3 Control Cell Extracts #9133
Phospho-Stat3 (Ser727) (6E4) Mouse mAb #9136A-431Serum-starve overnight, hEGF (100 ng/ml for 5–10 min)
Phospho-Stat3 (Ser727) Antibody #9134A-431Serum-starve overnight, hEGF (100 ng/ml for 5–10 min)
Acetyl-Stat3 (Lys685) Antibody #2523Transfected†Cotransfect with contructs expressing Stat3 and CBP (p300/CREB Binding Protein)
Phospho-Stat4 (Tyr693) (D2E4) Rabbit mAb #4134NK-92Cytokine-starve overnight, IL-2 (10 ng/ml for 15 min); IL-2 (5 ng/ml) should be included during growth
Phospho-Stat4 (Tyr693) Antibody #5267NK-92Cytokine-starve overnight, IL-2 (10 ng/ml for 15 min); IL-2 (5 ng/ml) should be included during growth
Phospho-Stat5 (Tyr694) (D47E7) XP® Rabbit mAb #4322HeLaSerum-starve overnight, IFN-α (100 ng/ml for 5 min)Stat5 Control Cell Extracts #9353
Phospho-Stat5 (Tyr694) (C71E5) Rabbit mAb #9314HeLaSerum-starve overnight, IFN-α (100 ng/ml for 5 min)Stat5 Control Cell Extracts #9353
Phospho-Stat5 (Tyr694) (C11C5) Rabbit mAb #9359HeLaSerum-starve overnight, IFN-α (100 ng/ml for 5 min)Stat5 Control Cell Extracts #9353
Phospho-Stat5 (Tyr694) (14H2) Mouse mAb #9356HeLaSerum-starve overnight, IFN-α (100 ng/ml for 5 min)Stat5 Control Cell Extracts #9353
Phospho-Stat5 (Tyr694) Antibody #9351HeLaSerum-starve overnight, IFN-α (100 ng/ml for 5 min)Stat5 Control Cell Extracts #9353
Phospho-Stat6 (Tyr641) (C11A12) Rabbit mAb #9364HeLaSerum-starve overnight, IL-4 (100 ng/ml for 15 min)
Phospho-Stat6 (Tyr641) Antibody #9361HeLaSerum-starve overnight, IL-4 (100 ng/ml for 15 min)
Phospho-Stathmin (Ser16) Antibody #3353HeLaNocadazole (100 ng/ml for 16 hr)
Phospho-Stathmin (Ser38) (D19H10) Rabbit mAb #4191HT-29Thymidine (2 mM for 16 hr) followed by Nocadazole (100 ng/ml for 24 hr)
Phospho-Stathmin (Ser38) Antibody #3426HeLaNocadazole (100 ng/ml for 16 hr)
Phospho-PLK Binding Motif (ST*P) (D73F6) Rabbit mAb #5243HeLaNocadazole (100 ng/ml for 16 hr)
Phospho-PKA Substrate (RRXS*/T*) (100G7E) Rabbit mAb #9624JurkatCalyculin A (100 nM for 30 min)Akt Control Cell Extracts #9273
Phospho-Thr-Pro-Glu (C32G12) Rabbit mAb #3004HeLaNocadazole (100 ng/ml for 16 hr)
Phospho-ATM/ATR Substrate (S*Q) (D23H2/D69H5) Rabbit mAb #9607293UV‡ (50 mJ/cm2), 30 min recovery
Phospho-(Ser/Thr) PDK1 Docking Motif (18A2) Mouse mAb #9634JurkatCalyculin A (100 nM for 30 min)Akt Control Cell Extracts #9273
Phospho-Threonine-Proline Mouse mAb (P-Thr-Pro-101) #9391JurkatNocadazole (1 µg/ml for 12 hr)
Phospho-(Ser/Thr) PKA Substrate Antibody #9621JurkatCalyculin A (100 nM for 30 min)Akt Control Cell Extracts #9273
Phospho-Threonine Antibody (P-Thr-Polyclonal) (HRP Conjugate) #6949NIH/3T3PDGF-BB (100 ng/ml for 5 min)
Phospho-(Ser/Thr) ATM/ATR Substrate Antibody #2851293UV‡ (50 mJ/cm2), 30 min recoverySAPK/JNK Control Cell Extracts #9253
Phospho-(Ser) Arg-X-Tyr/Phe-X-pSer Motif Antibody #2981JurkatCalyculin A (100 nM for 30 min)
Phospho-Survivin (Thr34) (D2E11) Rabbit mAb #8888HeLaThymidine (2 mM for 16 hr) followed by Nocadazole (100 mg/ml for 24 hr)
Phospho-Syk (Ser297) Antibody #14140SRTPA (200 nM for 15 min)
Phospho-Syk (Tyr323) Antibody #2715RamosAnti-IgM (12 µg/ml for 2 min)
Phospho-Syk (Tyr525/526) (C87C1) Rabbit mAb #2710RamosAnti-IgM (12 µg/ml for 2 min)
Phospho-Syk (Tyr525/526) Antibody #2711RamosAnti-IgM (12 µg/ml for 2 min)
Phospho-Synapsin-1 (Ser605) (D4B9I) Rabbit mAb #88246mouse brain, rat brainUntreated
Phospho-Synapsin (Ser9) Antibody #2311PC-12NGF (100 ng/ml for 2 hr)
Phospho-TAB2 (Ser372) (D5A4) Rabbit mAb #8155293Untreated
Phospho-TACC3 (Ser558) (D8H10) XP® Rabbit mAb #8842HT-29Thymidine (2 mM for 16 hr), add fresh media and Nocadazole (100 ng/ml 24 hr), collect by mitotic shake off
Phospho-TACC3 (Ser558) Antibody #5645HT-29Thymidine (2 mM for 16 hr), add fresh media and Nocadazole (100 ng/ml 24 hr), collect by mitotic shake off
Phospho-TAK1 (Thr184/187) (90C7) Rabbit mAb #4508HeLahTNF-α or IL-1β (20 ng/ml), both with Calyculin A (100 nM for 10 min)
Phospho-TAK1 (Thr184) Antibody #4537HeLahTNF-α or IL-1β (20 ng/ml), both with Calyculin A (100 nM for 10 min)
Phospho-TAK1 (Thr184/187) Antibody #4531HeLahTNF-α or IL-1β (20 ng/ml), both with Calyculin A (100 nM for 10 min)
Phospho-TAK1 (Thr187) Antibody #4536HeLahTNF-α or IL-1β (20 ng/ml), both with Calyculin A (100 nM for 10 min)
Phospho-TAK1 (Ser412) Antibody #9339K-562Untreated
Phospho-Talin (Ser425) (D2P2M) Rabbit mAb #13589293T
Phospho-Talin (Ser425) Antibody #5426293Untreated
Phospho-Tau (Thr181) (D9F4G) Rabbit mAb #12885mouse brain, rat brainUntreated
Phospho-Tau (Ser202) Antibody #11834Mouse brain, rat brain, human cerebellum
Phospho-Tau (Ser396) (PHF13) Mouse mAb #9632Mouse brain, rat brainUntreated
Phospho-Tau (Ser400/Thr403/Ser404) Antibody #11837mouse brain, rat brainUntreated
Phospho-Tau (Ser416) (D7U2P) Rabbit mAb #15013mouse brain, rat brainUntreated
Phospho-TBC1D1 (Thr590) Antibody #6927Transfected†Transfect with construct overexpressing TBC1D1
Phospho-TBC1D1 (Ser660) Antibody #6928Transfected†Transfect with construct overexpressing TBC1D1
Phospho-TBC1D1 (Ser700) Antibody #6929Transfected†Transfect with construct overexpressing TBC1D1
Phospho-TBK1/NAK (Ser172) (D52C2) XP® Rabbit mAb #5483THP-1TPA (80 nM overnight), LPS (1 µg/ml for 8 hr)
Phospho-TCTP (Ser46) Antibody #5251HT-29Thymidine (2 mM for 16 hr), add fresh media and Nocadazole (100 ng/ml 24 hr), collect by mitotic shake off
Phospho-Threonine (42H4) Mouse mAb #9386NIH/3T3PDGF-BB (100 ng/ml for 5 min)
Phospho-Threonine Antibody (P-Thr-Polyclonal) #9381NIH/3T3PDGF-BB (100 ng/ml for 5 min)
Phospho-Tie2 (Tyr992) Antibody #4221Transfected†293 cells transfected with wild-type Tie2
Phospho-Tie2 (Ser1119) Antibody #4226Transfected†Sf9 cells overexpressing GST-human Tie2 kinase domain fusion protein
Phospho-TIF1β (Ser824) Antibody #4127293Etoposide (30 µM for 3 hr)
Phospho-TLK1 (Ser695) Antibody #4121HeLaHydroxyurea (2 mM overnight)
Phospho-TNK1 (Tyr277) (D46E7) Rabbit mAb #5638L-540Untreated (L-540 cells express a 58 kDa TNK1-C17orf61 fusion protein containing 466 amino acids from the amino terminus of TNK1)
Phospho-TNK1 (Tyr277) Antibody #4553L-540untreated (L-540 cells express a 58 kDa TNK1-C17orf61 fusion protein containing 466 amino acids from the amino terminus of TNK1)
Phospho-Topoisomerase IIα (Ser1469) (D4F5) Rabbit mAb #13072293TUntreated
Phospho-Torc1/Crtc1 (Ser151) Antibody #3359Transfected†Transfect with construct overexpressing Torc1/Crtc1
Phospho-Tpl2 (Ser400) Antibody #4491Transfected†Transfect with a construct overexpressing Tpl2
Phospho-TPOR (Tyr626) (D3H7B) Rabbit mAb #12712M-07eThrombopoietin (100 ng/ml for 10 min)
Phospho-TRAF2 (Ser11) (E2B6L) Rabbit mAb #13908THP-1TPA (80 nM overnight) plus LPS (1 µg/ml for 30 min)
Phospho-TrkA (Tyr490) Antibody #9141PC-12NGF (100 ng/ml for 2–5 min)
Phospho-TrkA (Tyr490)/TrkB (Tyr516) (C35G9) Rabbit mAb #4619PC-12NGF (100 ng/ml for 2–5 min)
Phospho-TrkA (Tyr785)/TrkB (Tyr816) (C67C8) Rabbit mAb #4168PC-12NGF (100 ng/ml for 2–5 min)
Phospho-TrkA (Tyr674/675)/TrkB (Tyr706/707) (C50F3) Rabbit mAb #4621PC-12NGF (100 ng/ml for 2–5 min)
Phospho-Troponin I (Cardiac) (Ser23/24) Antibody #4004Primary cardiac myocytesIsoproterenol (1 µM for 5 min)
Phospho-TTF-1 (Ser327) Antibody #13608NCI-H3225, TT, H441
Phospho-Tuberin/TSC2 (Ser939) Antibody #3615NIH/3T3PDGF (50 ng/ml for 30 min)
Phospho-Tuberin/TSC2 (Ser1254) Antibody #3616MCF7Untreated
Phospho-Tuberin/TSC2 (Ser1387) Antibody #5584SHY5YUntreated
Phospho-Tuberin/TSC2 (Thr1462) (5B12) Rabbit mAb #3617MCF7hEGF (10 ng/ml for 30 min)
Phospho-Tuberin/TSC2 (Thr1462) Antibody #3611MCF7hEGF (10 ng/ml for 30 min)
Phospho-Tuberin/TSC2 (Tyr1571) Antibody #3614NIH/3T3PDGF (50 ng/ml for 30 min)
Acetyl-α-Tubulin (Lys40) (D20G3) XP® Rabbit mAb #5335HeLaTSA (400 nM for 16 hr)
Acetyl-α-Tubulin (Lys40) (D20G3) XP® Rabbit mAb (HRP Conjugate) #14652HeLaTSA (400 nM for 16 hr)
Acetyl-α-Tubulin (Lys40) (6-11B-1) Mouse mAb #12152HeLaTSA (400 nM for 16 hr)
Acetyl-α-Tubulin (Lys40) Antibody #3971HeLaTSA (400 nM for 16 hr)
Phospho-Tyk2 (Tyr1054/1055) Antibody #9321HeLaSerum-starve overnight, IFN-α (100 ng/ml for 15 min)
Phospho-Tyrosine (P-Tyr-1000) Rabbit mAb #8954JurkatPervanadate (1 mM pervanadate for 30 min)
Phospho-Tyrosine (P-Tyr-1000) MultiMab™ Rabbit mAb mix (HRP Conjugate) #12695A431Serum starved / 100 ng/ml hEGF for 5 minEGF Control Cell Extracts #5634
Phospho-Tyrosine Mouse mAb (P-Tyr-100) #9411JurkatPervanadate (1 mM for 30 min)
Phospho-Tyrosine Mouse mAb (P-Tyr-100) (HRP Conjugate) #5465JurkatPervanadate (1 mM for 30 min)
Phospho-Tyrosine Mouse mAb (P-Tyr-102) #9416JurkatPervanadate (1 mM for 30 min)
Phospho-Tyrosine Hydroxylase (Ser40) Antibody #2791PC-12Forskolin (30 µM for 5 min)MEK1/2 HeLa Control Cell Extracts #9160
Phospho-Tyrosine Hydroxylase (Ser31) (D6I9V) Rabbit mAb #13041PC-12Untreated
Phospho-Tyrosine Hydroxylase (Ser31) Antibody #3370PC-12Untreated
Phospho-ULK1 (Ser317) (D2B6Y) Rabbit mAb #12753MCF7, MEFCCCP (100 µM for 2 hr)
Phospho-ULK1 (Ser467) Antibody #4634Transfected†Untreated
Phospho-ULK1 (Ser555) (D1H4) Rabbit mAb #5869MCF7Oligomycin (0.5 μM for 30 min)
Phospho-ULK1 (Ser638) (D8K9O) Rabbit mAb #14205A172, MCF7Untreated
Phospho-ULK1 (Ser757) (D7O6U) Rabbit mAb #14202A172, MCF7Untreated
Phospho-ULK1 (Ser757) Antibody #6888A172, MCF7Untreated
Phospho-VASP (Ser157) (D1C8O) Rabbit mAb #84519A-431, NIH-3T3
Phospho-VASP (Ser157) Antibody #3111A-431Serum-starve overnight, Forskolin (10 µM for 30 min)
Phospho-VASP (Ser239) Antibody #3114A-431Serum-starve overnight, Forskolin (10 µM for 30 min)
Phospho-VEGF Receptor 2 (Tyr1059) (D5A6) Rabbit mAb #3817HUVECSerum-starve overnight, VEGF (100 ng/ml for 5 min)VEGF Receptor 2 Control Proteins #2904
Phospho-VEGF Receptor 2 (Tyr951) (15D2) Rabbit mAb #4991HUVECSerum-starve overnight, VEGF (100 ng/ml for 5 min)VEGF Receptor 2 Control Proteins #2904
Phospho-VEGF Receptor 2 (Tyr951) (7H11) Mouse mAb #2476Transfected†CKR/PAE cells expressing chimeric receptors containing human CSF-1 extracellular binding domain/mouse VEGFR-2 intracellular domainsVEGF Receptor 2 Control Proteins #2904
Phospho-VEGF Receptor 2 (Tyr951) Antibody #2471HUVECSerum-starve overnight, VEGF (100 ng/ml for 5 min)VEGF Receptor 2 Control Proteins #2904
Phospho-VEGF Receptor 2 (Tyr996) Antibody #2474Transfected†CKR/PAE cells expressing chimeric receptors containing human CSF-1 extracellular binding domain/mouse VEGFR-2 intracellular domainsVEGF Receptor 2 Control Proteins #2904
Phospho-VEGF Receptor 2 (Tyr1175) (19A10) Rabbit mAb #2478HUVECSerum-starve overnight, VEGF (100 ng/ml for 5 min)VEGF Receptor 2 Control Proteins #2904
Phospho-VEGF Receptor 2 (Tyr1175) (D5B11) Rabbit mAb #3770HUVECSerum-starve overnight, VEGF (100 ng/ml for 5 min)VEGF Receptor 2 Control Proteins #2904
Phospho-VEGF Receptor 2 (Tyr1212) (11A3) Rabbit mAb #2477Transfected†CKR/PAE cells expressing chimeric receptors containing human CSF-1 extracellular binding domain/mouse VEGFR-2 intracellular domainsVEGF Receptor 2 Control Proteins #2904
Phospho-Vimentin (Ser39) Antibody #13614PANC-1, ZR-75
Phospho-Vimentin (Ser56) (D5H2) Rabbit mAb #7391HeLaPaclitaxel (100 nM for 20 hr)
Phospho-Vimentin (Ser56) Antibody #3877HeLaPaclitaxel (100 ng/ml for 20 hr)
Phospho-Vimentin (Ser83) (D5A2D) Rabbit mAb #12569HeLaPaclitaxel #9807 (100 nM, G2/M arrested
Phospho-Vimentin (Ser82) Antibody #3878C2C12, C6Untreated
Phospho-Wee1 (Ser642) (D47G5) Rabbit mAb #4910A-431Serum-starve overnight, hEGF (100 nM for 5 min)
Phospho-WIPI2 (Ser413) Antibody #13571Raji, KARPAS-299Serum-starve overnight, TPA (200 nM for 30 min)
Phospho-WNK1 (Thr60) Antibody #4946HT-29Serum-starve overnight, hIGF-I (100 ng/ml for 30 min)
Phospho-YAP (Ser127) (D9W2I) Rabbit mAb #13008Hela, MEF, C6, C2C12
Phospho-YAP (Ser127) Antibody #4911HeLaUntreated
Phospho-YAP (Ser397) (D1E7Y) Rabbit mAb #13619Hela, MEF, C6, C2C12
Phospho-YB1 (Ser102) (C34A2) Rabbit mAb #2900MCF7Serum-starve overnight, hIGF-I (100 ng/ml for 30 min)
Phospho-Zap-70 (Tyr493) Antibody #2704JurkatAnti-CD3 (10 µg/ml for 2 min)
Phospho-Zap-70 (Tyr319)/Syk (Tyr352) (65E4) Rabbit mAb (PE Conjugate) #14791Jurkat, RamosAnti-CD3 (10 µg/ml for 2 min) or anti-IgM (12 µg/ml for 2 min)
Phospho-Zap-70 (Tyr319)/Syk (Tyr352) (65E4) Rabbit mAb #2717JurkatAnti-CD3 (10 µg/ml for 2 min)
Phospho-Zap-70 (Tyr319)/Syk (Tyr352) Antibody #2701JurkatAnti-CD3 (10 µg/ml for 2 min)
Phospho-Zyxin (Ser142/143) (D1E8) Rabbit mAb #8467A-431Untreated
Phospho-Zyxin (Ser142/143) Antibody #4863A-431hEGF (100 ng/ml for 30 min)
Phospho-Sin1 (Thr86) (D4U9L) Rabbit mAb #14716MCF7Serum-starve overnight +hIGF-1 (100 ng/ml for 10 min)p70 S6 Kinase MCF7 Control Cell Extracts #34499
Propionyl-Lysine [Prop-K] (D3A9R) Rabbit mAb #15159293TSodium-Propionate (Na-Prop, 50 mM; 16 hr)
Phospho-Ser-Pro-Pro Motif [pSPP] Rabbit mAb #14390HCT 116G0 cells (-): serum starved and contact inhibited (36 hr). Mitotic cells (+) thymidine (2 mM for 18 hr) followed by Nocadazole (50 ng/ml for 20 hr)
Phospho-Ser-Pro-Pro Motif [pSPP] Rabbit mAb #14390HCT 116Thymidine (2 mM for 18 hr) followed by Nocadazole #2190 (50 ng/ml for 20 hr)

† Antibody detects transfected levels of target protein.
‡ UV‡ treatment performed with a Stratalinker UV‡ Crosslinker.