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Multiplex Solutions for Spatial Biology

SignalStar™ multiplex immunohistochemical analysis of paraffin-embedded head and neck carcinoma.

Fast, Reliable Multiplex Spatial Insights at Scale


Fuel your next breakthrough with a diverse, validated portfolio of low- to high-plex solutions compatible with the sample types, platforms, and workflows used for multiplex spatial biology and multiplex imaging.

We’ve partnered with leading technology platforms to further streamline your research workflows and provide enhanced flexibility, efficiency, and reproducibility.

Visit Partnerships & Licensing to learn more.

Primary Antibody-Fluorophore Conjugates

Get fast results with off-the-shelf conjugates for commonly used targets.

Validated for use in FFPE tissue & frozen tissue.

Examples of compatible spatial biology platforms and workflows:


Secondary Antibody-Fluorophore Conjugates

Pair with primary unconjugated antibodies for increased signal-to-noise ratio.

Validated for use in frozen tissue.

Examples of compatible spatial biology platforms and workflows:

  • COMET System by Lunaphore
  • Cyclic Immunofluorescence (CyCIF)
  • Sequential Immunofluorescence (SeqCIF)

Find unconjugated primary antibodies validated for use in:

FFPE tissue

Frozen tissue


SignalStar® Multiplex IHC

Generate 8-plex results in 2 days with maximum signal-to-noise for accurate target detection.

Validated for use in FFPE tissue.

Easy-to-use panel design matches antibodies, fluorophores, and imaging rounds to create flexible and highly-validated antibody panels.

Automated protocols and compatible autostainers:

  • BOND RX and BOND RXm Automated Stainers by Leica Biosystems
  • ONCORE PRO X System by Biocare Medical
  • Spatial Station by Parhelia Bio

Chimeric Antibodies

Multiplex with ease by integrating recombinant monoclonal antibodies in different host species into existing protocols.

Validated for use in frozen tissue.

More host species to choose from that use the same trusted clones to detect proteins of interest.

Compatible workflows and products:

  • Seamless integration into your IF-frozen multiplexing workflow
  • Fully validated and compatible secondary antibodies available

Custom Antibody Conjugation & Labeling Services

Antibody conjugates to match your preferred multiplex spatial biology platforms.

  • CST frozen- and FFPE-validated antibodies to fluorescent dyes, oligonucleotides, and more
  • Partner with CST scientists on custom antibody conjugation and labeling

Customized & Carrier-Free Formulations

Conjugation-ready antibodies provided in carrier-free formulations.

  • Conjugation-ready formulations for assay platform compatibility
  • BSA- and azide-free at ≥1 mg/ml in 100 µl
  • Partner with CST scientists on custom formulations and formats

High-Performing & Flexible Solutions for Multiplex Spatial Biology


Image showing SignalStar multiplex immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma.

Multiplex spatial biology enables the detection of multiple protein targets simultaneously to reveal cellular dynamics within native spatial context, leading to a more comprehensive understanding of complex biological processes and systems.

Antibody-based techniques like multiplex immunofluorescence and multiplex immunohistochemistry are widely used to detect and visualize how different cell populations behave within their spatial context. These spatial workflows are complex, time-consuming, and costly to conduct. Antibody-based detection assays are only as good as the specificity of the antibodies used. Unreliable antibodies increase the risk of experiment failure, inaccurate and uninterpretable results, and squander limited tissue samples.

Maximize resources and get fast, reliable insights at scale with validated, flexible multiplex solutions for spatial biology across all your workflows.

Why Choose CST for Multiplex Spatial Biology


Reproducibility: Generate reliable, reproducible results and standardize your workflows—across users, platforms, and institutions—with rigorously validated antibodies and assays that guarantee functionality, specificity, and sensitivity.
Compatibility: Explore a diverse portfolio of validated multiplex solutions for spatial biology compatible with popular instruments, platforms, and methods that offer flexibility to match your current workflows and adapt as your research evolves.

Visit Partnerships & Licensing to learn more about our technology platform partners. 
 
Optimize Workflows and Resources: Save time, resources, and valuable samples. Our application-specific testing minimizes the validation and optimization required, and automated staining platform compatibility reduces hands-on time. Streamline assay development with intuitive and easy-to-use assay design tools.
Scientific Expertise and Support: Efficiently design your complex spatial biology experiments with guidance from CST experts and scientists. From troubleshooting technical issues to partnering with us to tailor solutions to your specific needs, you’ll get the right support from a company of dedicated scientists.
Reliable Supply: Keep your spatial biology projects on track with lot reservations and bulk orders for the amount and project timeline you need. More than 99% of products are available for immediate shipping.

Learn How CST Can Support Your Assay Design


Improve the quality and speed of your assay development and experiments. CST Assay Design Tools facilitate assay optimization and empower you to focus on your results with these resources:

  • Fluorescence Spectra Viewer
  • Flow Cytometry Panel Builder
  • SignalStar Multiplex IHC Panel Builder

Multiplex Spatial Biology FAQs

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