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In the CST video library, you can watch how-to videos from the Tech Tips series to improve your technique, research webinars presented by scientists from CST and other institutions, and videos relevant for specific applications or disease research areas.

The CST protocol for Fluorescent Multiplex Immunohistochemistry allows you to characterize 6 or more targets in one sample with flexible experimental design by employing a serial labeling strategy. In this video, Jen highlights five key protocol steps you can optimize to help get a balanced set of signals.
5 Tips to Start Your Multiplex IHC Optimization
How sample type, processing steps, and characteristics of the target protein can impact detection and inform protocol optimization in immunofluorescence.
A Guide to Detecting Your Favorite Protein by IF
This presentation recorded at SLAS describes the use of antibodies to probe targets in the TGF-β/Smad Signaling pathway using high throughput, automated quantitative ELISA and fluorescence-based microscopy assays.
A Multiscale Approach to Quantitatively Evaluate the SMAD Signaling Pathway
Dr. Gygi describes Bioplex, a project to systematically map protein interactions. Knowing these interactions can provide insight into a protein's biological function, place it within signaling networks, and generate mechanisms and hypotheses underlying biological processes.
A Protein Interaction Network Created From Thousands of Protein Immunopurifications
SignalStar® Multiplex IHC for spatial biology. Up to 8-plex protein detection generated in just 2 days. Watch the on-demand webinar.
Accelerate Spatial Discoveries Up to 70% Faster with SignalStar® Multiplex IHC
Exploring the plasticity of tumor-associated macrophages (TAMs) and challenges in distinguishing M1- versus M2-polarized macrophages.
Advances and Challenges for Understanding Macrophages in the Tumor Microenvironment
Dr. Reuben Shaw discusses advances in AMPK and autophagy signaling, identification and characterization of novel AMPK substrates including ULK1/Atg1, and the potential for ULK1 inhibitors in cancer therapeutics.
Advances in AMPK and Autophagy Signaling
Watch this 3-D animation to see how flow cytometry enables the simultaneous characterization of intracellular signaling states and phenotyping of rare cell populations in a heterogeneous sample.
An Introduction to Flow Cytometry
This presentation showcases the use of antibodies immunofluorescence and flow cytometry, and the importance of validation.
Analysis of Cellular Signaling by Fluorescent Imaging and Flow Cytometry
Watch this webinar to learn about a streamlined workflow for generation of CD19 CAR-T cells using BioTechne analytical instrumentation and anti-CAR linker antibodies from CST.
Analytical Tools to Evaluate CAR-T Cell Signaling & Activation
Visualize immune cell identity, function, and spatial context with CST’s validated IHC antibodies. Maximize your research impact with precision tools.
Analyze Immune Cell Phenotype & Function
Is the performance an unconjugated antibody enough to validate a conjugated version of the same clone for your assay? Not necessarily! Learn why in this video.
Antibody Conjugates: What You Need to Know
Our continuing goal is to make the highest quality, most consistent antibodies that work the first time, every time.
Antibody Validation at CST
Answers to common questions about antibody validation and the roles of both the vendors and the scientists who use them in their research.
Antibody Validation Q&A
Before you start your next immunofluorescence (IF) experiment, ask these three questions to ensure you will get reliable results and avoid headaches: 1) Is your antibody specific? 2) Is your antibody supported by an optimized IF protocol? 3) Is your antibody performing consistently?
Ask these 3 questions before your next IF experiment
Learn how phosphorylation, ubiquitination, & Atg8ylation impact autophagy, a key cellular process linked to health & disease. Watch the on-demand webinar featuring Reuben Shaw & Vojo Deretic.
Autophagy's hidden drivers: Exploring the role of post-translational modifications
This video highlights considerations for optimizing antibodies and adjusting the protocols when transfering protocols from manual to automated immunohistochemistry.
Autostainer IHC Protocol Transfer Q&A
Meet Gary Kasof PhD, who over his career has developed some of the most-cited antibodies antibodies for studying cell death. Gary also shares advice for young scientists.
Behind the Antibodies with Gary Kasof
You probably know that a highly specific, high affinity primary antibody is key to a successful immunohistochemistry. Companion reagents such as buffers are just as important. We will describe an example of our experience with protocol optimization and highlight the role of Antigen Retrieval for IHC.
Better IHC Intro and Step 1: Antigen Retrieval
Good IHC protocols provide the proper conditions for a specific binding event to occur. It is therefore essential that you calibrate the ionic strength and pH of the antibody diluent to the specific antigen-epitope interaction you are trying to detect.
Better IHC Step 2: Antibody Dilution
Detection reagents bind to the primary antibody, either directly or indirectly, through a secondary antibody intermediate. When a chromogenic substate is introduced, a precipitate forms that deposits at the site of the primary antibody antigen binding event.
Better IHC Step 3: Detection
In designing your experiment, choose a chromogen that produces a color with appropriate contrast to your counterstain, and of a high enough intensity to reveal the antigen you are trying to detect.
Better IHC Step 4: Chromogen
Watch this webinar for applications, tips, and tricks for Simple Western, for fast, reliable, and hands-free automated western blotting using CST antibodies. See an overview of the CST-Bio-Techne partership, brining westerns into the 21st century.
Bringing the Western Blot into the 21st Century with Automated Simple Western – A Customer's Perspective
The success of your ChIP experiment relies on the antibody you choose. This video describes the different validation approaches our scientists use to ensure the antibodies we recommend for ChIP-qPCR and ChIP-seq will perform as you expect.
Can You Trust Your ChIP Results?
How does antibody validation affect your IHC results? Learn about the approaches scientists at CST use to validate antibodies for IHC in this video: cell pellets, multiple tissues, and treatments with activators, inhibitors, or blocking peptides.
Can You Trust Your Immunohistochemistry Results?
This video describes the application of robust multiplex imaging technologies to interrogate myeloid and lymphoid lineages to provide spatial insights of many cell types in the tumor microenvironment.
Capturing the Spatial Landscape of Tumor and Immune Cell Lineages in the Microenvironment of Human Cancer Tissues
Keep your cell and tissue culture free of contamination - watch this how-to video for tips and advice from our scientists.
Cell Culture: Aseptic/Sterile Technique
Dr. Nathanael Gray of the Stanford Cancer Institute discusses efforts to synthesize and characterize small molecule degraders of cancer targets, including kinases and transcriptional regulators.
Challenges and Opportunities in Protein Degradation
Combining analysis of surface markers and intracellular signaling readouts through multiplexed flow cytometry adds power to your experiments. This can present challenges when designing antibody panels. This video walks through an example experiment to illustrate how antibodies can be selected to assess activation of T cell subpopulations.
Characterizing Immune Responses using Flow Cytometry
1.ChIP和ChIP seq的区别和应用范围； 2.ChIP和ChIP seq 实验的操作步骤和成功要素； 3.酶解和超声的优缺点及如何正确选择； 4.如何选择合适的用于ChIP和ChIP seq的抗体； 5.CST 标准 DNA文库建立方法及ChIP-Seq测序数据分析解读
ChIP & ChIP-Seq实验原理与成功要素及NGS测序数据分析解读
Need to decide between different lysis buffers for your Western Blot (WB)? Before you start running your proteins with RIPA buffer, cell lysis buffer, or SDS, Sarah has some questions you should consider.
Choosing a Lysis Buffer for Western Blot (WB)
Learn how to select antibodies with application-specific validation to ensure reproducibility and success in your IF, IHC, and multiplex imaging assays.
Choosing the Right Antibody for Imaging Success
Learn how the optimal crosslinking time in Chromatin Immunoprecipitation (ChIP) can be influenced by sample type, and by target protein (histone, transcription factor, or cofactor). Longer crosslinking time is beneficial in certain situations, but over-crosslinking can make it harder to shear genomic DNA. Fang breaks it down in this Tech Tip.
Chromatin Crosslinking: How Much Time? Chromatin Immunoprecipitation (ChIP)
Watch this video to learn how CST® CUT&Tag kits, reagents, and antibodies fuel your research with consistent, reliable, and reproducible results.
Chromatin Profiling Just Got Faster with CUT&Tag
Cigall Kadoch PhD keeps a running list of targets to advance mechanistic studies of human disease. Here, Dr. Kadoch discusses the value of collaborating with CST to develop novel reagents to probe ATP-dependent chromatin remodeling complexes.
Cigall Kadoch, PhD: Science is a Team Sport
As the importance of the mammalian SWI/SNF (BAF) complexes in contributing to cancer became apparent, Dr. Kadoch realized there was a real lack of reagents to effectively study this class of proteins.
Cigall Kadoch, PhD: Specific, High Affinity Antibodies for Discovery
To help cancer researchers to push their field forward, CST scientists dig into the literature to identify unmet needs. Dr. Cigall Kadoch shares her thoughts on what makes CST antibody development and validation unique in this video.
Cigall Kadoch, PhD: What Makes CST Unique
This video explains the roles of the bait and prey proteins in co-IP, and applications of immunoprecipitation for studying protein-nucleic acid interactions.
Co-IP Bait and Prey, and other applications of Immunoprecipitation (IP)
Watch this video to learn how two different methods (antibody-based immunoaffinty enrichment and IMAC) combine for improved coverage of the phosphoproteome.
Complementary Technologies for Phospho-Peptide Enrichment
Learn how spatial biology and multiplex IHC can be used to understand the immune system's role in disease and therapy. This webinar covers assay design, antibody selection, and methods for various plex sizes.
Context is Key: Leveraging Spatial Biology in Immunology and Immunotherapy
This presentation explores multidimensional, image-based methods for immunophenotyping of cancer immune microenvironments and the design of antibody panels for high-plex platforms.
Critical Considerations For Optimizing Imaging Mass Cytometry
Video: the updated CUT&RUN kit provides improved results, confirmed compatibility with fixed frozen cells, tissues, and primary cells, uses even fewer cells.
CST CUT&RUN Kit: Validated for Flexibility
该视频重点介绍肿瘤微环境当中缺氧代谢与血管生成相关的调节分子和信号通路，并为各位听众提供在上述领域的主要评价指标作为参考。
CST肿瘤微环境主题系列讲座---第一讲：缺氧代谢与血管生成
该视频将重点介绍与细胞骨架、细胞基质动态调控及EMT相关的主要调节分子和信号通路，并为各位听众提供在上述领域的主要评价指标作为参考。
CST肿瘤微环境主题系列讲座---第三讲：细胞粘附、细胞骨架、细胞基质、EMT
该视频重点介绍肿瘤免疫微环境相关的主要参与细胞、调节分子和信号通路，并为各位听众提供在上述领域的主要评价指标和新的研究方法作为参考。
CST肿瘤微环境主题系列讲座---第二讲：肿瘤免疫微环境
Drug discovery and development can incorporate proteomics studies which enable accelerated target identification and deeper insights of mechanism of action and off-target effects.
Customized Proteomics Solutions for Expedited Drug Discovery
A comprehensive, step-by-step video of the CUT&RUN Protocol, from sample preparation to CUT&RUN DNA purification and qPCR quantification. Expert tips for critical steps and experimental design are highlighted to help you successfully complete the assay.
CUT&RUN Protocol: A Visual Guide
Learn how CUT&RUN can be performed in as little as 1-2 days using low cell number samples, and how CUT&RUN has been employed to study epigenetic remodeling and alternative splicing directed by the NF-κB pathway downstream of a viral oncoprotein.
CUT&RUN: A New Way to Analyze Epigenetic Marks and Mechanisms
Accumulation of senescent cells is considered a hallmark of aging and can drive many age-related pathologies including dementias, arthritis, atherosclerosis, and others.
Deciphering Aging: Linking Senescence with DNA Damage and the Cell Cycle
Tumor heterogeneity is a major clinical challenge and is driven by DNA damage and genomic instability. This webinar describes several mechanisms of tumorigenesis caused by DNA damage, including changes to copy number, and discusses combinatorial therapeutics that increase replication stress in order to halt cancer cell division and tumor growth.
Deciphering Cancer: Genomic Instability in Cancer
This webinar will examine how, by targeting proteins responsible for the crosstalk between epigenetics and metabolism, we may be able to develop new and effective therapeutic options for cancer treatment.
Deciphering Cancer: The Intersection of Epigenetics, Metabolism, and Tumorigenesis
Understanding the functional mechanisms and signaling pathways that regulate activation of specific T cell subpopulations is crucial to optimizing effectiveness of the next generation of CAR-T therapies. Presented by Wendell Lim and Stanley Riddell.
Deciphering CAR-T Cells: Exploring Functional Mechanisms to Drive Next-Generation Immunotherapy
Drs. Schietinger and Youngblood review the underlying mechanisms and epigenetic programs determining tumor-specific T-cell dysfunction and reprogrammability and discuss current challenges and outstanding research questions in cancer immunotherapy.
Deciphering epigenetic regulation of the immune response in cancer
Uncovering the mechanisms underlying RNA modifications and RBP function are emerging frontiers in medicine that take the study of gene regulation a step beyond epigenetics
Deciphering Epitranscriptomics: The importance of RNA modifications and RNA-binding proteins in disease
Understanding the underlying biology of fibrosis is critical for the diagnosis, treatment, and management of cardiac fibrosis, nonalcoholic steatohepatitis (NASH), and other fibrotic diseases
Deciphering Fibrosis: Drivers of Fibrotic Disease in the Liver and Heart
Key signaling pathways modulate immune cell activation and tumor checkpoint evasion. Improved understanding of tumor microenvironment crosstalk and mechanisms of response/reistance will be key for next-generation cancer immunotherapies.
Deciphering Immuno-Oncology: Targeting Cellular Mechanisms of the Tumor Immune Response
This webinar will focus on how therapeutic interventions targeting dendritic cells can promote tumor immunity and enhance the efficacy of cancer therapy.
Deciphering Immuno-Oncology: Targeting Innate Immunity in Cancer
Drs. Michael Heneka and Christian Haass present recent findings on how signaling involving TREM2 in microglia triggers the innate immune response in neurodegenerative diseases including Alzheimer's.
Deciphering Microglia: Exploring Cellular Pathways in Neurodegenerative Diseases
This webinar will focus on the potential role of innate immunity in neurodegeneration and cognitive function, particularly in Alzheimer's disease.
Deciphering neurodegeneration: Inflammation, immune response, and Alzheimer's
Drs. Gary Schwartz and J. Julian Blow discuss advances in understanding CDKs and how CDK inhibitors can be used in combination with cancer therapeutics.
Deciphering the cell cycle: The role of cell cycle control in cancer
Drs. Michael Keebler and Chaolin Zhang present work on protein-RNA dynamics in neuronal synapses and RNA splice regulation in neuronal development.
Deciphering the Role of RNA-Binding Proteins in Neurodevelopment and Disease
Innovative anti-linker monoclonal antibodies from CST eliminate the need to develop unique detection reagents for every new chimeric antigen receptor (CAR) molecule developed, speeding detection in flow cytometry panels.
Detecting CAR Expression with Versatile, Innovative Antibodies
A comprehensive, step-by-step video of the CUT&RUN DNA Library Prep Protocol for Illumina systems, picking up after the CUT&RUN protocol video. Key protocol steps and tips are highlighted.
DNA Library Prep for CUT&RUN: A Visual Guide
This video highlights the role of histone modifications and epigenetic regulation in driving progression of breast cancer. Targets include, Estrogen Receptor, Ezh2, UTX, and HK27Me3.
Epigenetic Drivers of Breast Cancer
This video highlights epigenetic regulators that contribute to progression of these blood cancers: mixed lineage leukemia, myelodysplastic syndrome, and acute myeloid leukemia. Here are several targets involved in epigenetics to consider for your MLL, MDS, or AML research: MLL1, p300/CBP, DOT1L, DNMT3A, TET2, and 5-methylcytosine (5-MC).
Epigenetic Drivers of Leukemias
Epigenetic regulators contribute to the progression of prostate cancer through altered androgen receptor signaling, as well as other mechanisms. This video introduces three epigenetic targets: Androgen Receptor, Glucocorticoid Receptor, and Ezh2.
Epigenetic Drivers of Prostate Cancer
The chromatin immunoprecipitation (ChIP) assay is a powerful technique used for probing protein-DNA interactions in studies of chromatin regulation and epigenetics. This webinar discusses important factors to consider when performing ChIP, including use of highly validated antibodies, optimized protocols and reagents, and the advantages of using enzyme-based chromatin digestion.
Epigenetics Analysis using Highly Validated Antibodies and Optimized ChIP Assays
Designing or troubleshooting flow cytometry experiments to analyze intracellular signaling? Here’s what you need to know.
Experimental Design: Analyzing Intracellular Signaling With Flow Cytometry
Good controls can help you get your experiment back on track if something goes wrong, and can help you have confidence in your results. In this Tech Tip video, Ginny goes over three types of controls for your immunofluorescence (IF) experiment: positive controls, endogenous controls, and negative controls.
Experimental Design: Controls for Immunofluorescence (IF)
This webinar provides practical advice to optimize flow cytometry protocols to reveal essential signaling insights needed to uncover the activity and function of rare immune cell subsets.
Feel the Flow: Immune Cell Signaling Cascades Reveal Phenotype and Function
Explore the difference between two major types of fixatives for immunofluorescence: crosslinking aldehydes including formaldehyde, and alcohols including methanol. John describes how choice of fixative can impact the structure of epitopes and the fluorescent properties of the sample itself, and affect your results.
Formaldehyde vs. Alcohol Fixation for Immunofluorescence (IF)
Should you switch from western blot to flow? We’ll go over situations where flow cytometry can take your research further in this Tech Tip video with Sarah.
Four Situations Where Flow Cytometry Can Help
To enable surface characterization of chimeric antigen receptors (CARs), scientists at CST developed versatile and specific antibodies that detect the linker sequence of scFv domains. Watch the poster presentation from AACR.
Generation and Validation of Anti-Linker Monoclonal Antibodies for the Surface Detection of scFv-based CARs
Learn how to boost sensitivity and reproducibility in protein detection to tackle low-abundance targets where traditional western blotting fails.
Going Beyond the Limits of Traditional Westerns
Imaging Mass Cytometry (IMC) is a novel technique used to visualize over 50 antibodies simultaneously on tissues with sub-cellular resolution. This webinar will reveiw this technique and how antibody quality impacts highly multiplexed techniques.
Highly Multiplexed Single Cell Analysis of Tumor Heterogeneity Through Time and Space by Mass Cytometry
This video describes the simplified PTMScan® HS workflow for LC-MS analysis of ubiquitination and SUMOylation that improves readout sensitivity, reduces sample prep time, and eliminates lyophilization, 9M urea, and antibody elution steps.
Highly Sensitive Post-Translationally Modified Peptide Enrichment for LC-MS Analysis
Learn about the history of Cell Signaling Technology and our commitment to providing reproducible tools to move your science forward.
History of CST
This animated explainer shows how the CUT&RUN method works for mapping protein-DNA interactions in native chromatin, while using fewer cells and saving you time.
How CUT&RUN Profiles Chromatin
Learn how choice of antibody diluent can affect your IHC results in this Tech Tip with Chris. Differences in antibody diluent properties can influence antibody-epitope interactions and specificity, and ultimately, the strength of your staining.
How Does Antibody Diluent Affect Staining in Immunohistochemistry (IHC)?
More antibody isn't always better! The optimal amount of antibody to use in a ChIP assay varies, depending on the antibody's affinity, sensitivity and specificity. See how the amount of antibody used can affect your Chromatin Immunoprecipitation (ChIP) results in this Tech Tip featuring Fang.
How Much Antibody Should I use in ChIP assays?
The success of your ChIP experiment depends on the fragmentation of chromatin, a critical step in the ChIP protocol. This can be accomplished with either sonication or enzymatic digestion. But how do you decide which chromatin fragmentation protocol to use in your ChIP experiments? This video will show you the way.
How to Choose Enzymatic or Sonication Protocol for ChIP
Optimizing chromatin fragmentation can impact the quality of ChIP-qPCR and ChIP-seq results. This video provides guidance on choosing a fragmentation protocol, ChIP-seq library QC, and troubleshooting ChIP-qPCR results.
How to Choose the Appropriate ChIP Protocol and Generate Reliable Data
If you’re only looking at surface markers in your flow cytometry, you’re missing out! We’ll discuss several protocol approaches to multiplexing so that you can analyze both surface markers and intracellular targets.
How to Combine Surface & Intracellular Targets in Flow Cytometry
Adding intracellular signaling targets to your flow cytometry analysis? You'll need to fix and permeabilize your cells to allow antibodies to access their epitopes. Rob explains the protocol variations.
How to Fix and Permeabilize Cells for Flow Cytometry
Selecting antibodies and a protocol for flow cytometry comes with several considerations which are discussed in this webinar.
How to Flow: Antibody Staining Walkthrough
Reliably identify senescence signaling in your samples using biomarkers with a 3-stage approach outlined in this video. No single assay is sufficient, but screening, co-staining, and verification with multiple biomarkers can improve your confidence.
How to Identify Senescence in Cells and Tissue
When setting up Immunohistochemistry (IHC) experiments, cell pellet controls can be used to test antibody specificity and tell you whether your protocol is working or needs to be adjusted. In this Tech Tip, Chris highlights situations where cell pellet controls can be valuable.
How to Incorporate Cell Pellet Controls in IHC Experiments
The goal of this video is to guide you through chromatin fragmentation, a critical step in a Chromatin Immunoprecipitation (ChIP) experiment. It is important to consider whether the target protein is a histone modification, transcription factor, or transcription co-factor since each protein binds differently to DNA. Due to these binding differences, additional measures should be taken into account when optimizing chromatin fragmentation for each protein class, whether performing sonication or enzymatic digestion.
How to Optimize your Chromatin Fragmentation for ChIP
Antigen retrieval can influence the strength of staining obtained in IHC. In this Tech Tip, Franny and Abigail show you why antigen retrieval (AR) is important for immunohistochemistry (IHC), and give you tips on how to set up Heat Induced Epitope Retreival in your microwave.
How To Perform Antigen Retrieval (AR) in Immunohistochemistry (IHC)
Protein enrichment by immunoprecipitation is frequently carried out prior to analysis. In this Tech Tip, Sarah presents considerations for choosing antibodies and beads for IP.
How to Select an Antibody for Immunoprecipitation
Chromatin fragmentation can be performed using either sonication or enzymatic digestion. Despite its name, the enzymatic protocol still requires brief sonication. In this Tech Tip, Fang shows you what to look for to ensure sufficient chromatin yield.
How to Sonicate Chromatin: Enzymatic ChIP Protocol
Chromatin fragmentation can be performed using either sonication or enzymatic digestion. This Tech Tip video covers the sonication protocol, in which sonication facilitates both cell and nuclear lysis as well as fragmentation of the chromatin. Varying the sonication time affects target enrichment and chromatin yield.
How to Sonicate Chromatin: Sonication ChIP Protocol
This video demonstrates the technique used for splitting adherent cell lines with trypsinization.
How to Split Adherent Cell Lines: Step-By-Step Demonstration
This video demonstrates the technique used for splitting suspension cell lines.
How to Split Suspension Cell Lines: Step-By-Step Demonstration
How do you know if your antibody is working properly in immunohistochemistry (IHC)? In this Tech Tip, Abbey discusses the use of positive and negative tissue controls, isotype controls, and controls for phospho-specific and modification-specific antibodies.
IHC Tissue Controls & More
帮助科研工作者更好地理解 IHC 在疾病诊断中的核心作用，通过应用实例讨论 IHC 技术操作过程、结果分析中的细节和注意事项。介绍 mIHC 的原理方法，并以研究实例讨论 mIHC 在疾病研究中的价值。
IHC 和 mIHC 在疾病研究诊断中的方法介绍及注意事项
This video provides a step-by-step guide to the immunohistochemistry protocol for paraffin-embedded tissue tissue sections, used by scientists in Cell Signaling Technology's IHC group.
Immunohistochemistry Protocol for Paraffin-Embedded Tissue Sections
Immunohistochemistry Protocol for Paraffin-embedded Tissue Sections (Japanese Audio)
Immunohistochemistry Protocol for Paraffin-embedded Tissue Sections (Japanese Audio)
Watch this video for a step-by-step demonstration of the Immunoprecipitation Protocol for Native Protein for Western Blot analysis. Includes guidance for experimental design, choosing antibodies and beads, and tips and tricks for successful protein IP.
Immunoprecipitation Protocol: A Visual Guide
Researchers have been investigating several potential avenues to counter tumor immunosuppression and re-activate the immune system. One of these is CAR-T therapy, which is designed to harness the potent cytotoxicity of T cells by re-engineering their surface receptors. This video gives an overview of this exciting field. What’s on the horizon for the CAR-T therapy field, and what challenges are researchers and clinicians going to tackle to enable the next success? We’ll explore this exciting frontier in part two of our CAR-T video series.
Immunotherapy and CAR-T Cells
In this video, our experts provide protocol guidance for lysate preparation steps prior to protein electrophoresis and western blotting.
Impacts of Lysis Buffer and Sonication on Western Blotting Outcomes
Discover advanced methods for studying TREM2 and downstream signaling in human-relevant iPSC-derived microglia to advance Alzheimer’s disease research.
Interrogating Cell Signaling Pathways in iPSC-Derived Microglia
Learn how InTraSeq™ Single Cell Analysis enables unbiased quantitation of RNA alongside intracellular and cell surface proteins at the single-cell level.
InTraSeq™ Single Cell Analysis: A Multimodal Single-Cell Method for Simultaneous Measurement of RNA and Proteins to Uncover New Single-Cell Biology
By combining single cell analysis of intracellular protein phosphorylation states with RNAseq, InTraSeq technology provides deeper understanding of cellular heterogeneity and function.
InTraSeq™ Single Cell Analysis: Get More Data from Your scRNAseq
This webinar will explore the idea that a better understanding of developmental biology signaling pathways will advance our understanding of adult tumors and cancer stem cells as well as boost our ability to create effective therapeutics to fight a broad array of cancers.
Key Signaling Pathways in Cancer - Links to Developmental Biology
Accelerated metabolism is a hallmark of both immune responses and cancer. This webinar describes the roles of aerobic glycolysis, lipid metabolism, and mitochondrial remodeling in immune metabolism, and explains how metabolic changes impact innate immunity.
Key Signaling Pathways in Cancer: Emerging Trends in Immunometabolism
What to know about widefield versus confocal imaging settings for your microscope to get optimal immunofluorescence data. What to know about widefield versus confocal imaging settings for your microscope to get optimal immunofluorescence data.
Learning Lab LIVE! Imaging Q&A with IF expert Virginia Bain, PhD
The CUT&RUN assay enables faster results from smaller samples to enable your analysis of protein-DNA interactions. Our CUT&RUN expert offers helpful protocol tips to get your CUT&RUN experiments running.
Learning Lab LIVE! Q&A with CUT&RUN Expert Fang Chen, PhD
How can intracellular and extracellular protein readouts be combined in flow cytometry? This video offers protocol advice from our flow expert.
Learning Lab LIVE! Q&A with Flow Cytometry Expert Robert MacDonald, PhD
Need to choose fluorophores and antibodies when designing your immunofluorescence experiment? Our immunofluorescence expert has some advice.
Learning Lab LIVE! Q&A with IF Expert Virginia Bain, PhD
Why are antigen retrieval and antibody diluent important for the immunohistochemistry protocol? Our IHC expert offers protocol advice.
Learning Lab LIVE! Q&A with IHC Expert Chris Grange
Antibody optimization in pilot experiments is recommended prior to running full-scale immunohistochemistry assays.
Learning Lab LIVE! Q&A with IHC Expert Jessica Simendinger
How does protein expression level factor in your ability to detect proteins and post-translational modifications by western blot?
Learning Lab LIVE! Q&A with Western Blot Expert Srikanth Subramanian, PhD
Drs. Cominelli and Kugathasan describe new techniques to identify and target inflammatory signaling in gastrointestinal immune cells in this webinar.
Listen to Your Gut: Deciphering the Role of Immune Cells in Inflammatory Gut Disorders
To characterize activation of immune cells, measurement of intracellular signaling using phospho-specific antibodies combined with immune phenotyping using surface markers is ideal. Owing to the unique capability of flow cytometry to measure events at a single-cell level, “Phospho Flow” allows for the identification of rare signaling events during processes such as co-stimulatory signaling and manipulation of checkpoint inhibition.
Mastering Phospho-Flow: Immune Cell Activation
Infiltrating myeloid cells are analyzed in the tumor microenvironment using multiplex IHC, employing new monoclonal antibodies to IDO1 and Arginase.
Mechanisms of Myeloid Immunosuppression: Functional Characterization of the Tumor Microenvironment Using Single and Multiplex IHC
How does choice of blocking protein - nonfat dry milk vs bovine serum albumen (BSA) - affect your Western Blot (WB) results? In this Tech Tip, Srikanth explains our protocol recommendations to reduce background caused by nonspecific binding.
Milk or BSA? Choosing a Blocking Protein for Western Blotting (WB)
How to combine modulators with selection of antibody targets and controls in western blot experiments.
Modulating Signaling Activities with Activators & Inhibitors for Western Blot Experiments
Optimizing the order of serial antibody labeling steps for fluorescent multiplex IHC can help you achieve a balanced set of signals. This Tech Tip video shows how to evaluate epitope sensitivity to guide antibody panel construction.
Multiplex IHC Optimization: Antibody Order
When designing an antibody panel for multiplex IHC, it’s important to optimize each antibody’s dilution and which fluorophore it’s paired with. This will help your staining achieve a balanced set of signals.
Multiplex IHC Optimization: Antibody Pairing & Titration
Advances in multiplex IHC using Opal and validated CST rabbit primary antibodies to reliably stain for 6 markers in a mouse model of breast cancer; using Akoya Bioscience's Phenoptics platform to perform quantitative analysis of tumor biology.
Multiplexing on Mouse tissue using CST antibodies and OPAL IHC method
Targeted protein degradation selectively alters protein abundance by co-opting the cellular degradation machinery. The degradation tag (dTAG) system can be used for target validation and mechanistic studies.
New Horizons for Protein Degradation: Immediate, Selective Protein Control Using the dTAG System
Unlock Th17 cellular mechanisms with InTraSeq single-cell analysis. Watch the webinar to learn about RNA & protein co-quantification & more.
No More (Data) Secrets: Unlock the Molecular Mechanisms of Pathogenic and Non-Pathogenic Th17 Cells
Rapidly advancing single-cell and multi-omic technologies generate complex datasets, but how can they drive deeper insights and actionable discoveries?
Not All Antibodies Are Created Equal: Why Application‑Specific Validation Defines Single‑Cell Insights
Watch this webinar to learn how the new CAR linker antibodies can interrogate the expression of an entire panel of CARs.
Novel Antibodies for the Characterization of CAR-T Cells
Webinar about Post-translational Modification Enrichment and Quantitation for Precision Medicine and Drug Development
Post-translational Modification Enrichment and Quantitation for Precision Medicine and Drug Development
PTMScan® from CST uses the K-e-GG ubiquitin remnant motif antibody, applying it to perform large-scale quantitative analysis of the ubiquitylome.
Post-translational Modification: Antibody Enrichment for Mass Spectrometry-based Proteomics
This webinar will focus on the use of simultaneous affinity enrichment workflows by combining different PTM antibodies for ‘one-pot enrichment’ approaches
Post-translational Modifications and Data-Independent Acquisitions - Challenges and Opportunities
Highly Multiplexed IHC Assays to Examine Immune Checkpoints and Biomarkers for Immunotherapy
Poster - Highly Multiplexed IHC Assays to Examine Immune Checkpoints and Biomarkers for Immunotherapy
This video illustrates how the CD133 (D4W4N) XP® Rabbit mAb was validated by CST. The D4W4N clone is approved for western blotting and IHC, and detects human CD133 protein with exceptional specificity and sensitivity. It outperforms the currently available CD133 antibodies in market, setting a new industry standard for the detection of CD133 protein.
Poster - Validation of a Glycosylation-Independent Antibody Against the Cancer Stem Cell Marker CD133
Quantitative analysis of the SMAD signaling pathway using ELISA, high content imaging, and high-resolution confocal approaches were used to study the regulation of angiogenesis and cell proliferation.
Poster: A Multiscale Approach to Quantitatively Evaluate the SMAD Signaling Pathway
In this video, our flow expert compares different cell dissociation methods and how to assess their effects on cell viability, protein expression, and signaling.
Preparing for Flow: Cell Dissociation Methods
To keep your projects moving forward, you need to count on reagents that will work as expected and be available throughout the lifetime of your study.
Protect Your Drug Discovery Pipeline
Webinar on mass spectometry used to investigate intracellular signaling activities via antibody enrichment of post-translational modifications
Proteomics Meets Cellular Signaling
This webinar will focus on the utility of the PTMScan method in early drug discovery and the future of automation in multiplexed analysis of cellular signaling.
Proteomics Profiling of Post-Translational Modifications in Early Drug Discovery
This video introduces PTMScan technology, a platform for the enrichment of post-translational modifications (PTMs) in mass spectrometry-based proteomics studies.
PTMScan: Proteomics of Post-translational Modifications
This video describes considerations for optimizing capture and detection antibody pairs to enable consistent ELISA results.
Reliable Results with Optimized ELISA
Reproducibility Issues in Life Science Research: Antibody Validation Challenges
Reproducibility Issues in Life Science Research: Antibody Validation Challenges
Dr. Reuben Shaw provides his perspective as a cancer researcher on the value of developing research tools including modification-specific and phosphorylation-specific antibodies, in collaboration with Cell Signaling Technology, and how those tools enable the research community to push forward and make new discoveries.
Reuben Shaw, PhD: How CST Fuels Discovery
Dr. Reuben Shaw discusses the collaborative relationship his lab has developed with scientists at Cell Signaling Technology. This collaboration has led to the development and validation of substrate motif antibodies that has enabled profiling of the signaling activities in metabolic diseases and cancers in Dr. Shaw’s lab and around the world.
Reuben Shaw, PhD: Partnering for Success with CST
What's the benefit of sonicating lysates before loading on a gel? If you don't perform sonication as part of your western blot workflow, you might want to check out this quick Tech Tip presented by Srikanth.
Should You Sonicate Your Western Blot Lysates?
This video shows how you can amplify up to 8 targets in mouse or human tissue in just 2 days, with flexible panel design and high-quality antibodies from CST.
SignalStar® Multiplex IHC for Spatial Biology
Watch this 20-minute webinar for tips and tools to optimize simultaneous incubation of different antibodies, plus insignts into maximing multiplexing efficiency while maintaining assay quality.
Simplified Staining: Signaling Analysis in Heterogeneous Populations by Flow Cytometry
Dr. David Rimm of Yale School of Medicine discusses multiplexing spatial imaging technologies and their development to improve response prediction for immunotherapy. This webinar was recorded at AACR 2022.
Spatial Imaging as a Tool for Discovery of New Candidates to Better Predict Response to Immunotherapies
This video highlights three essential tips for splitting mammalian cell cultures, and explains how to use ratios or a cell counter to when propagating cells.
Splitting Cells: Why It's Important and 3 Essential Tips
Drs. Benjamin Wolozin and Dorothee Dormann present recent research on phase separation mechanisms regulating stress granule proteins implicated in ALS, frontotemporal dementia, and other neurodegenerative diseases.
Stress granules: Deciphering the connections to neurodegeneration
Speakers discuss the assembly and biological architecture of mammalian SWI/SNF complexes and the role of cancer-specific SWI/SNF complex mutations (present in 20% of cancers) in driving oncogenicity. Also, novel approaches toward the identification of small molecule therapeutics for this class of human tumors and the development and validation of new high quality recombinant monoclonal antibodies for ChIP and ChIP-seq. And finally, the important factors to consider for optimizing different types of proteins—including histones, transcription factors, and cofactors—for ChIP.
Structure and Function of Epigenetic Regulators in Human Disease
Understanding the activity of intracellular signaling pathways are crucial to developing therapies that maximize efficacy and minimize toxicity. Mass spectroscopy-based proteomics and intracellular flow cytometry can further our understanding of immune regulation in cancer and improve therapeutic potentials.
Studying Immune Regulation: Global Phosphorylation Profiling and Flow Cytometry
By selectively targeting previously inaccessible oncogenic proteins for degradation, researchers are enabling functional studies with superior kinetic resolution and discovering new therapeutic targets.
Targeted protein degradation: Controlling and assessing therapeutic targets to advance oncology drug discovery
This webinar explores how aberrant histone modifications can affect the expression of key genes and contribute to the pathological progression of cancer. Specific epigenetic pathways are candidates for therapeutic intervention.
Targeting Cancer Pathways: The Epigenetics Question
The abnormal tumor microenvironment fuels oncogenic progression and treatment resistance. By better understanding stromal/tumor interactions and remodeling of surrounding matrix and blood vessels, we can develop more effective treatment options, including immunotherapy.
Targeting Cancer Pathways: The Tumor Microenvironment
This webinar explores how cancer cells reprogram their metabolic pathways to increase energy production. The roles of metabolic changes, autophagy, and cell death in influencing tumor progression, and potential therapeutic interventions are considered.
Targeting Cancer Pathways: Tumor Metabolism and Proliferation
Some cancers develop resistance to drugs that target signaling pathways and become increasingly aggressive. By understanding how cancer cells re-wire their signaling, more effective clinical strategies leveraging new and existing therapies can be designed.
Targeting Cancer Pathways: Tumor Resistance
Tumors exploit immune checkpoint pathways to evade detection and destruction by the immune system. Learn how immunotherapy re-activates the immune system to kill cancer cells by targeting PD-1 / PD-L1, CTLA-4, and other targets.
Targeting Cancer Pathways: Understanding Immune Checkpoints
An overview of oh mass spectrometry-based proteomics studies can be a powerful tool for drug discovery and development
Targeting Proteomics to Decipher Biology for the Drug Development Pipeline
Know what to look for and dig deeper into the validation data for your antibody to avoid the frustration of ambiguous or misleading results.
The CST Hallmarks of Validation
Watch this webinar on the critical role of antibody validation in experimental success. Learn strategies to ensure immunoassay reproducibility and optimize your protocols.
The Importance of Antibody Validation and Its Impact on Your Research
In this presentation, we'll cover different IHC controls to determine if your observed staining (or lack thereof) is accurate and the antibody is specific.
The Importance of Controls in Immunohistochemistry
The complexity of the proteome makes discovery research seem like trying to find a needle in a haystack. Learn how enrichment with PTMScan decreases sample complexity and increases the likelihood that you'll find what you're looking for.
The Potential of Proteomics: A Clear Path to Discovery (Part I)
Learn how PTMScan technology facilitates translational discovery, identification of disease drivers, and development of markers and treatments, as exemplified in the study of non-small cell lung cancer (NSCLC).
The Potential of Proteomics: A Clear Path to Discovery (Part II)
CST has always believed that the only way to produce and validate our products is to do it in-house. That way, we can guarantee that they are rooted in sound science, and that our antibodies will be specific and reproducible tools available to research scientists.
The Story of an Antibody, Whiteboard Edition
Watch this video to learn how epitope tags can be leveraged in your experiments when your protein of interest is newly discovered, expressed at low abundance, or poorly immunogenic.
The Utility of Epitope Tags in Western Blot and Immunoprecipitation
Learn insider tips and tricks for successful SignalStar® Multiplex IHC experiments. This video covers critical steps, troubleshooting, and optimization for both manual and automated protocols to help you maximize experimental success and confidently interpret your results.
Tips & Tricks for Successful SignalStar® Experiments
What antibodies can be used in a CUT&RUN protocol? What controls should be included to measure signal-to-noise? These and other frequently asked questions are answered in this edition of CST Tech Tips.
Top 5 Frequently Asked CUT&RUN Questions
Does your immunofluorescence (IF) look like a bright mess? In this edition of Tech Tips, Ginny talks about what too much signal means, potential causes, and solutions for this problem.
Troubleshooting Immunofluorescence: Excess Bright Signal
This video presents tips to help your immunoprecipitation experiment: how to avoid IgG masking; optimizing epitope tags; experimental design (controls); what to consider when choosing lysis buffers, antibodies, and beads.
Validating Protein Interactions with Co-Immunoprecipitation Using Endogenous and Tagged Protein Models
Microglia, critical mediators of neuroinflammation, are shown in the context of a whole mouse brain by probing with anti-Iba1/AIF1.
Visualizing Microglia in Whole-Tissue Cleared Mouse Brain
本次研讨会将通过介绍CST Western Blot标准操作步骤，向您阐释WB操作中的关键点及注意事项，特别是影响最终结果呈现的样本制备和抗体孵育与检测。通过真实失败案例，如无信号、高背景、条带大小不正确、条带弯曲拖尾等，分析WB常见的问题及原因，并提供相应的解决策略。
WB标准操作流程与常见问题解答
Why is antibody validation important? How can vendors, researchers, and journals improve the reproducibility of research? This webinar addresses these questions and highlights considerations for selecting antibodies and designing experiments.
Webinar - Achieving Reproducibility: Don't let Antibodies be a Variable in your Experiment
The reproducibility of scientific research is a rising concern in both academia and industry. This webinar will highlight how antibody specificity affects reproducibility in biomedical research and identify key requirements for proper antibody validation.
Webinar - Antibody Validation Roundtable: Specificity + Sensitivity + Reproducibility = Validation
This webinar discusses the application of both traditional IHC and new tissue processing methods in transgenic mouse models of Alzheimer’s disease. These methods enable whole-brain analysis and insights into molecular and neural circuit mechanisms underlying Alzheimer’s disease.
Webinar - Immunolabeling for Neurodegenerative Diseases
Multiplex immunohistochemistry (mIHC) supports detection and spatial characterization of multiple (>6) proteins in preserved tissue. Learn how mIHC enables tumor immunology and immune checkpoint studies.
Webinar - Mulitplex IHC and Novel Monoclonal Antibodies as Enabling Tools for the Study of Tumor Immunology
Lysate controls for Western Blot (WB) experiments are from a sample with known expression of your protein of interest, giving you an idea of how much signal to expect from your experimental samples. In this Tech Tip, Alex discusses how to use positive lysate controls, how they differ from loading controls, and considerations for phospho-specific antibodies.
Western Blot (WB) Experimental Design: Choosing Positive Controls
Common problems encountered in western blotting include low signal or high background. This video helps you troubleshoot problems in your Western Blot experiment, tips to help diagnose problems, and provides solutions to ensure you get the expected results in the shortest amount of time.
Western Blot Troubleshooting Guide
Western Blot Troubleshooting Guide (Japanese Audio)
Western Blot Troubleshooting Guide (Japanese Audio)
Western Blot Troubleshooting Guide (CN)
Western Blot Troubleshooting Guide 实验操作步骤视频解析
Western blotting (WB) is widely used to analyze specific protein expression in cell or tissue extracts. We understand that western blotting experiments are time consuming and critical to your research progress. For that reason, we provide optimized protocols along with reference information and technical support to make your western blotting experience successful.
Western Blotting Protocol
Western Blotting Protocol (Japanese Audio)
Western Blotting Protocol (Japanese Audio)
Western Blotting Protocol (CN)
Western Blotting Protocol 蛋白印迹实验步骤
Studies of neurodegeneration using mouse models of Alzheimer's disease can now employ whole-brain tissue clearing techniques to better visualize expression of β-Amyloid and other hallmarks of disease.
Whole-Brain Imaging of β-Amyloid Staining with Tissue Clearing Techniques
To develop effective therapeutics and vaccines to fight the COVID-19 pandemic, solid experimental work using reliable, validated reagents and antibodies are as important as ever.
Why Antibody Validation is Crucial for SARS-CoV-2 Research
This presentation gives an overview of the chromatin immunoprecipitation (ChIP) technique and three keys to success: optimized protocol & quality reagents, chromatin crosslinking & fragmentation, and validation.
Your Guide to a Successful ChIP Assay
Western Blot Basic Webinar (JP)
ウェスタンブロッティング (WB) の基礎
ChIP Basic Webinar (JP)
クロマチン免疫沈降 (ChIP) の基礎
Immunohistochemistry Basic Webinar (JP)
免疫組織化学染色 (IHC) の基礎
Immunofluorescence Basic Webinar (JP)
免疫蛍光染色 (IF) の基礎
本讲座将为大家系统地介绍cGAS-STING信号通路在天然免疫和肿瘤免疫研究领域的广泛作用，以及作为干预靶标的巨大潜力。
具有巨大潜力的天然免疫和肿瘤免疫干预靶标——cGAS-STING信号通路
本场讲座将逐一介绍多种细胞程序性死亡（凋亡、自噬、程序性坏死、细胞焦亡）相关信号通路关键蛋白和研究策略，并就多种细胞程序性死亡形式之间的联系和转化展开讨论，为大家全面认识细胞程序性死亡和开展系统研究提供参考。
凋亡相关信号通路研究进展和关键蛋白研究策略
本讲座全面讲解如何深入开展信号通路的研究，从大数据挖掘到锁定目标信号通路，进一步探讨疾病分子机制，并涉及到相关主流的实验技术，如修饰蛋白质组数据库使用、WB、ChIP、IHC、PTMScan蛋白质翻译后修饰高通量筛选技术等。
如何让你的发现可靠可重复？千里之行始于足下
作为指导方针，本次讲座将与您分享CST流式细胞术专家在日常的试验工作中经常使用的经验和技巧。
掌控Phospho-Flow： 巧用细胞内、外流式细胞术优化免疫细胞活化分析
随着流式细胞术近几年的飞速发展，它已成为生物科学领域最有力的分析技术之一。对于这一技术，有的人却望尘莫及，问题层出不穷，比如，胞内染色染不出，找不到合适的抗体，修饰蛋白不知道怎么刺激等等。其实，跳出墨守陈规的流式应用，深度挖掘更深层次的流式实验，助力科研及临床的发展，是所有 Flower 为之奋斗的目标。本讲座将带您了解 Cell Signaling Technology (CST) 流式产品在细胞通路研究中的独到之处，让我们一起在广阔的流式世界中，携手同行，共辟蹊径。
流式（Flow）入通路，蹊径何处寻 | Webinar
Immunohistochemistry Protocol for Paraffin-embedded Tissue Sections (CN)
用于石蜡包埋的组织切片的免疫组织化学实验步骤 | Immunohistochemistry Protocol for Paraffin-embedded Tissue Sections
本讲座将概述细胞代谢相关的信号通路，并举例说明系列重要细胞代谢研究靶点，和精彩研究案例思路
细胞代谢相关信号通路概览，重要靶点和研究案例分享
细胞焦亡与其他多种细胞程序性死亡形式之间存在相互联系和转化，共同构成细胞程序性死亡的复杂体系，维持细胞和机体的内稳态。
细胞焦亡信号通路的关键蛋白和研究动态
细胞程序性死亡(Programmed Cell Death, PCD)，是指细胞接受某种信号或受到某些因素刺激后，为了维持内环境稳定而发生的一种主动性消亡过程。它
细胞程序性死亡相关信号通路关键蛋白和研究策略
该视频将将逐一介绍参与自噬信号通路的关键分子，包括ULK1 、Beclin1、LC3、Atg5，ATG12，P62等
自噬相关信号通路关键蛋白和研究策略
本场 Webinar 详细介绍 PTMScan® 技术，并结合精准医学中的应用案例介绍如何选择和使用 PTMScan® 相关产品。
蛋白翻译后修饰组学技术（PTMScan®）在精准医学中的应用