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Western Blot (Immunoblot)

Western Blot (Immunoblot)

Western blotting is a widely used technique to detect levels of protein expression in cell or tissue extract. This technique measures protein levels in a biological sample through antibody binding to a specific protein of interest.

Our rigorous validation protocols help us provide specific and reliable antibodies, which helps you get reproducible and meaningful data every time.

We offer a comprehensive list of companion products that are validated in-house with our protocols so that you can get all of the reagents you need to complete your experiment.

Companion Products for Western Blotting:

Western Blotting Gel

Western blot of extracts from various cell lines, untreated or treated as indicated with LY294002 #9901 (a PI3 kinase inhibitor), Human Insulin-like Growth Factor I (hIGF-I) #8917 (which activates Akt via IGFIR binding), or Human Platelet-Derived Growth Factor AA (hPDGF-AA) #8913 (which induces the PI3K/Akt pathway through receptor binding), using Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb #4060 (left) and Akt (pan) (C67E7) Rabbit mAb #4691 (right).