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Immunofluorescence Protocol with Gelatin Blocking Step: easy to follow directions describing the step by step experimental procedure.
Nuclear Permeabilization Staining: easy to follow directions describing the step by step experimental procedure.
Flow Cytometry Protocol - Extracellular Staining - Conjugated Secondary Abs: easy to follow directions describing the step by step experimental procedure.
Immunofluorescence Protocol without Permeabilization: easy to follow directions describing the step by step experimental procedure.
Immunofluorescence Protocol with n-Octyl-Glucoside Permeabilization: easy to follow directions describing the step by step experimental procedure.
Immunofluorescence Protocol with Saponin Permeabilization: easy to follow directions describing the step by step experimental procedure.
Immunofluorescence Protocol with DOTMAC Permeabilization: easy to follow directions describing the step by step experimental procedure.
List of publications based on primary research done by the Cancer Research Group at CST (2002-2013).
We currently have four different secondary antibodies approved for use in fluorescent western blotting, depending on species and wavelength of detection:
Listing of IHC-related scientific posters and papers involving scientists from Cell Signaling Technology.
Immunohistochemistry (IHC) is a powerful technique. Learn how to perform IHC on paraffin sections with our step-by-step IHC protocol. Click here.
Immunofluorescence Protocol – Specific for Product: 2187: easy to follow directions describing the step by step experimental procedure.
In-Cell Western Protocol: easy to follow directions describing the step by step experimental procedure.
Co-staining with multiple antibodies is a low-content form of multiplex analysis that be achieved using different host species or fluorophore conjugates.
Scientific posters presented by CST scientists at various conferences and trade shows through the years.
A comprehensive list of peer-reviewed publications from Cell Signaling Technology.
Immunofluorescence Protocol for Biotin-Conjugated Antibodies: easy to follow directions describing the step by step experimental procedure.
Jak and cytokine receptor mutations found in various cancers, along with the corresponding PubMed reference(s).
Immunofluorescence Protocol for Labeling with BrdU Antibody: easy to follow directions describing the step by step experimental procedure.
KinomeView Protocol: easy to follow directions describing the step by step experimental procedure.
A chronological listing of case studies and peer-reviewed publications utilizing PTMScan proteomic technology developed by Cell Signaling Technology.
ELISA-Peptide Assay Protocol : easy to follow directions describing the step by step experimental procedure.
Immunohistochemistry Protocol (Paraffin): easy to follow directions describing the step by step experimental procedure.
Immunohistochemistry Protocol (Paraffin) - Specific For Products: 2085, 3197: easy to follow directions describing the step by step experimental procedure.
Immunohistochemistry Protocol: easy to follow directions describing the step by step experimental procedure for product #12692.
A scientific resource for the MBT protein domain containing information on structure, function, and binding to methyl-lysine motifs during chromatin regulation.
Western blot procedure, from separating proteins on a gel to transferring them to a membrane and detecting them with antibodies. Click here.
Flow Cytometry BrdU Modified Protocol: easy to follow directions describing the step by step experimental procedure.
Western Blotting Protocol (Fluorescent): easy to follow directions describing the step by step experimental procedure.