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Expert-reviewed diagram providing a current overview of the m6A RNA pathway with references to its role in tumor development
Hallmarks of Antibody Validation-Complementary Strategies that Employ Western Blot, Immunohistochemistry (IHC), Immunofluorescence (IF/ICC), Flow Cytometry, Histone Antibody, ChIP-qPCR, ChIP-seq.
Complementary strategies provide vital information regarding antibody specificity or functionality and can be tailored to the nature of the downstream assay.
Guest blogger Dr Gregory discusses research to define the epitranscriptome, including modifications that increase tRNA stability and prevent degradation.
Learn how RNA-binding proteins influence RNA regulation and epitranscriptomics and contribute to the progression of a variety of diseases.
There is still much to learn about the hidden layer of gene regulation—the chemical modification of RNA. What is the effect of m6A, the most abundant mRNA modification observed in eukaryotes?
Streamline your neurodegeneration therapeutic development with CST recombinant monoclonal antibodies, ELISA and cellular assay kits, custom products, and services.
Streamline your oncology therapeutic development with CST recombinant monoclonal antibodies, ELISA and cellular assay kits, custom products, and services.
The PI3K / Akt Substrates Table provides a comprehensive list of demonstrated downstream targets of Akt phosphorylation.
CST offers flow validated antibodies for single cell mass cytometry (MC) and IHC validated antibodies for imaging mass cytometry (IMC)
Quantitating Ser/Thr phosphorylation by Immobilized Metal Affinity Chromatography enrichment and analysis identifies thousands of sites with minimal sample.
PTMScan® Glycosylation
Watch this video to learn how two different methods (antibody-based immunoaffinty enrichment and IMAC) combine for improved coverage of the phosphoproteome.
ELISA Protocol for PathScan Stress and Apoptosis Signaling Antibody Array Kit: easy to follow directions describing the step by step experimental procedure.
PTMScan videos and links to complementary technologies for phospho-peptide enrichment and Science Webinar on Proteomics and Cell Signaling.
For years, the TME could only be viewed in single-cell snapshots by flow cytometry or limited tissue staining. IMC offers a panorama of this dynamic world.
Validated antibodies and kits from CST for your researchevaluating the hallmarks of neurodegeneration,neurodegenerative disorders, and neural function.
Immunoaffinity purification of PTM peptides for mass spectrometry-based proteomics--PTMScan
A scientific resource for the NZF protein domain containing information on structure, function, and domain binding to ubiquitin.
Our MT1-MMP (D1E4) Rabbit mAb #13130 was produced with an antigenic peptide corresponding to residues surrounding Met293 of human MT1-MMP protein (see UniProt ID #P50281; https://www.uniprot.org/uniprot/P50281). Therefore, the antibody recognizes both the full-length MT1-MMP zymogen and the proteolytically processed, active enzyme (residues 112 to 582). The 62 kDa band represents the inactive zymogen that includes the inhibitory prodomain. MT1-MMP is activated by cleavage between Arg111 and Tyr112 to produce a mature/active enzyme [see Golubkov V.S. et al. (2007) J Biol Chem 282, 36283-91 (PMID: 17938169; https://www.ncbi.nlm.nih.gov/pubmed/17938169)] that migrates at approximately 50 kDa. We have not performed any direct testing to confirm that the 50 kDa band is definitively the cleaved form, but it is very likely the case based on the literature, expression levels, and epitope location.
For PTMScan® enrichments, we recommend a liquid chromatography (LC) gradient length of 90 minutes and replicate injections for each enrichment. Therefore, four PTMScan enrichments will have a total of eight liquid chromatography-mass spectrometry (LC–MS)/MS runs. For the immobilized metal affinity chromatography (IMAC) based phosphopeptide enrichments, the sample complexity requires a longer gradient length of 120 minutes, also performed with replicate injections for each sample.
Webinar | Highly Multiplexed Single Cell Analysis of Tumor Heterogeneity through Time and Space by Mass Cytometry
CST® Proteomics Analytical Services help you identify and validate drug targets, discover biomarkers, determine on/off-target effects, and mechanisms of action.
Streamline Targeted Protein Degradation Drug Discovery with specific, application-validated antibodies and industry-leading proteomic services.
Humans have been creatively reusing materials for centuries. As our global waste problem grows, we must be more creative and diligent in finding solutions.
Authors of an article in Science Signaling investigated the effects of a Myc tag insertion location on antibody function. Their efforts focused on the most cited antibody for Myc, monoclonal antibody 9E10.
A recent publication highlights the advantages and disadvantages of different approaches to multiplex IHC/IF and explains how these methods differ.
DIY conjugation kits let researchers label an antibody themselves but lengthy protocols, poor conjugate quality and low yield cause inconsistent results.
