Resources results for "Notch 1"

TMT Labeling and Detection

TMT Labeling and Detection

Developmental Biology Signaling

Overview of stem cell markers, lineage markers, development and differentiation signaling networks at Cell Signaling Technology.

Pluripotency and Differentiation

Learn more about stem cell pathways and the transcription factors Oct-4, Sox2, and Nanog are essential for maintaining the pluripotent state of stem cells.

Angiogenesis

Expert-reviewed interactive pathway providing a current overview of angiogenesis pathways and links to products by Cell Signaling Technology.

Download Pathways by Disease Area

Resources include signaling pathways, antibodies and companion products, research overviews, technical resources, and recent review articles.

Ubiquitin Ligase Table

The Ubiquitin Ligase Table provides a comprehensive list of E3 ubiquitin ligases along with their substrates and corresponding PubMed reference(s).

Neural and Glial Cell Markers

There are a number of markers that can be used to distinguish the many cell types of the central and peripheral nervous system during development, adult neurogenesis, and the pathogenesis of neurodegenerative disorders.

Something to YAP About! Progress in Targeting YAP/TAZ-TEAD Signaling in Cancer Research

Breakthroughs in therapeutic targeting of YAP/TAZ-TEAD signaling using small molecules were presented at AACR 2023. Read a recap of the presentations.

Neuroscience

Expert-reviewed interactive neuroscience pathways providing current overviews of Parkinson's and Alzheimer's Disease signaling, as well as links to products from CST.

Validated CST® Antibodies Work on the Cell DIVE Multiplexed Imaging Solution

Matched Antibody Pairs

Find validated CST® matched antibody pairs and streamline the development of your High-Throughput ELISA-Like Immunoassays for screening.

Cell Signaling Technology Joins 1% for the Planet

We have committed to donating 1% of our total annual revenue to environmental giving in collaboration with 1% for the Planet. Join the movement.

Resources

We do not perform enzymatic activity assays internally using the Cell Lysis Buffer (10X) #9803, so we are unable to guarantee that the enzymatic activity will be conserved. We routinely use this buffer in immunoassays such as western immunoblot, IP, and ELISA without issue.

As a reference, the components of the Cell Lysis Buffer (10X) #9803 are provided below:
20 mM Tris-HCl (pH 7.5), 150 mM NaCl, 1 mM Na₂EDTA, 1 mM EGTA, 1% Triton, 2.5 mM sodium pyrophosphate, 1 mM beta-glycerophosphate, 1 mM Na₃VO₄, 1 µg/ml leupeptin

Resources

We typically recommend the PathScan® Sandwich ELISA Lysis Buffer (1X) #7018 that comes with the Glucose-6-Phosphate Dehydrogenase (G6PD) Activity Assay Kit #12581 as it has been validated for use with the kit. The PathScan® Sandwich ELISA Lysis Buffer (1X) #7018 was made specifically for this assay kit and for ELISA formats. We have not tested any other lysis buffers with the Glucose-6-Phosphate Dehydrogenase (G6PD) Activity Assay Kit #12581, so we cannot guarantee the performance. The ingredients of the PathScan® Sandwich ELISA Lysis Buffer (1X) #7018 are as follows: 20 mM Tris-HCl (pH 7.5), 150 mM NaCl, 1 mM Na2 EDTA, 1 mM EGTA, 1% Triton, 20 mM sodium Pyrophosphate, 25 mM Sodium Fluoride, 1 mM β-glycerophosphate, 1 mM Na3VO4, 1 ug/ml leupeptin.

Resources

CIP/Lambda Phosphatase Treatment of Cell Lysates:

1) Wash cells 2X with ice-cold PBS.

2) Add cold 1X cell lysis buffer that does not include phosphatase inhibitors [1-2 E7 cells per ml or 0.4ml per 10 cm plate (80-90% confluent)]. Our cell lysis buffer is prepared as follows: