Resources results for "9860"
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X-Gal can be tricky to solubilize, therefore we recommend dissolving in DMF (#12767) as listed on the Senescence β-Galactosidase Staining Kit (#9860) datasheet. However, we have tested and verified that DMSO (#12611) can be used to successfully dissolve the X-Gal, and therefore we consider it to be a suitable replacement for DMF.
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While we have not tested the Senescence β-Galactosidase Staining Kit #9860 with frozen tissue in-house, we have spoken with numerous customers who have done so with success using our recommended cell staining protocol. One adaption for frozen tissue which we recommend is to use a chamber slide which will allow you to fully submerge your tissue sections and prevent evaporation. Here is a link to an example of different chamber slides. The amount of staining solution you use will be determined by the chamber size you have. It is important to keep your samples fully submerged when you incubate overn…
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We have not tried counterstaining in-house, however, the paper below mentions using Hoechst as a fluorescent DNA dye to quantify the cells:
Baker, D. J. et al. (2004) BubR1 insufficiency causes early onset of aging-associated phenotypes and infertility in mice. Nat. Genetics 36, 744-749
You may be able to find other references on the web, as this kit is popular and there are many publications citing its use. We use hematoxylin routinely in our IHC experiments, and, consequently, have limited experience with alternatives.
For further suggestions, it may be helpful to seek out information within the Histonet (http://www.histosearch.com/histonet.html). This is a well-monitored discussion forum for histology professionals. There is an archive of queries that can be searched, or a new query can be posted. There may be suggestions or observations using these methods that could prove helpful.