Resources results for "alpha-tubulin antibody"

Products (31)
Resources (87)

Resources

Based on sequence homology, we predict that our α-Tubulin Antibody #2144 and α-Tubulin (11H10) Rabbit mAb #2125 will detect the alpha 1a, alpha 1b, alpha 1c, alpha 2, alpha 3c, alpha 3d, alpha 3e, alpha 4a, and alpha 4b subtypes.

Regulation of Microtubule Dynamics

Expert-reviewed interactive pathway providing a current overview of Regulation of Microtubule Dynamics.

Neural and Glial Cell Markers

There are a number of markers that can be used to distinguish the many cell types of the central and peripheral nervous system during development, adult neurogenesis, and the pathogenesis of neurodegenerative disorders.

Loading Control Expression in Mouse Tissues

When it comes to mouse tissue samples, not all loading control proteins are expressed equally. Learn which control is right for your research.

Resources

Based on homology, our β-Tubulin Antibody #2146, β-Tubulin (9F3) Rabbit mAb #2128, β-Tubulin (D3U1W) Mouse mAb #86298, and β-Tubulin (D2N5G) Rabbit mAb #15115 should detect β-Tubulin isoforms A, B, and C. The antigen sequences for these antibodies have 96% homology with beta 2C, beta 4B, beta 5B, and beta 4A isoforms 1, 2, and 3, and 95% homology with beta 2C isoforms A and B. Therefore they are likely to detect these subtypes.

Cytoskeletal Regulation and Vesicle Trafficking

Expert-reviewed interactive signaling pathways providing current overviews of the regulation of actin and microtubule dynamics, as well as links to products from CST.

How do I choose a loading control for my western blot?

Loading controls are essential to ensure data reliability in your western blot. Learn about the three main considerations for loading control selection.

Neuronal, Astrocyte, and Oligodendrocyte Markers

The key to identifying neurons, microglia, oligodendrocytes, and astrocytes lies in using antibodies that target protein biomarkers specifically expressed and localized within these cells.

PTMScan® Ser/Thr Kinases

Information and case study data for the PTMScan Direct Ser/Thr Kinases Service, which allows for the targeted screening of a defined set of protein modification sites.

Loading Control Antibodies

Ensure equal sample loading with loading control antibodies from CST.

Organelle Markers

Organelle markers for immunofluorescence analysis from CST Cell Signaling Technology

Antibody Validation for Western Blotting

The accuracy of western blot results relies heavily on the quality of the primary antibody used. CST™ antibodies are validated using multiple experiments.

Resources

Our Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb #4060 and Phospho-Akt (Ser473) (D9W9U) Mouse mAb #12694 detect over-expressed PKC alpha and beta. Our Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb #4060 also detects endogenous levels of PKC in Jurkat + CalA extract, but not in other endogenous models tested. Our Phospho-Akt (Ser473) (587F11) Mouse mAb #4051 detects over-expressed PKC alpha and beta but does not detect endogenous PKC in the models tested. Our Phospho-Akt (Ser473) (193H12) Rabbit mAb #4058 does not detect over-expressed or endogenous PKC alpha and beta.

Characterizing the Autophagic-Lysosomal Pathway in Parkinson’s Disease

These antibodies to CNS markers can help study how LRRK2 mutations impact the autophagic-lysosomal pathway (ALP) and contribute to Parkinson's disease.

Resources

Our Phospho-Acetyl-CoA Carboxylase (Ser79) (D7D11) Rabbit mAbs #11818 and #42801 detect both phospho-ACC-alpha and phospho-ACC-beta. These antibodies recognize ACC-alpha only when phosphorylated at Ser80 in human, which corresponds to Ser79 in mouse and rat. They also recognize ACC-beta only when phosphorylated at Ser222 in human, corresponding to Ser212 in mouse and Ser218 in rat.

Resources

From our testing, we have seen that our Glucocorticoid Receptor (D6H2L) XP® Rabbit mAb #12041 detects both the alpha and beta isoforms of the Glucocorticoid Receptor. It is possible that #12041 may detect other less common isoforms as well, but we have not confirmed this experimentally. Additionally, each of the isoforms that can be detected most likely have post-translational modifications that affect the molecular weight.

Our Glucocorticoid Receptor (D8H2) XP® Rabbit mAb #3660 detects both the alpha and beta isoforms of the Glucocorticoid Receptor. However, based on sequence alignment (and unlike #12041) #3660 is predicted to cross-react with all known alternative translation start site generated isoforms of glucocorticoid receptor-α and glucocorticoid receptor-β (80 kDa).

Please refer to Uniprot ID: P04150 for the reference sequences of both alpha and beta isoforms of the Glucocorticoid Receptor.

Resources

Unfortunately, we are not aware if any of our amyloid-beta antibodies will detect the alpha-oligomer of the amyloid-beta protein.

Resources

PI4 Kinase Antibody #4902 detects endogenous levels of total PI4 Kinase isoform 230. In humans, PI4K230, PI4K92, and PI4K55 are four PI4 kinase isoforms that have been characterized and classified according to their molecular weights of 230, 92, and 55 kDa. In the literature, alpha refers to the 230 kDa size band (UniProt ID: P42356) and beta refers to the 92 kDa size band (UniProt ID:Q9UBF8). Type 2 alpha (UniProt ID: Q9BTU6 ) and type 2 beta (UniProt ID: Q8TCG2) refer to the 55 kDa band.

Unravelling Parkinson’s Disease: The Role of Mitophagy, Autophagy & Lysosomal Processing

PD is characterized by functional loss in dopaminergic neurons, believed to be caused by disruption of proteins involved in cell quality control pathways.

Subcellular Marker Selection for Immunofluorescence

There are many reasons to include a subcellular marker in your IF experiment: studying organelle-specific functions, assessing drug effects, and more.

PTMScan® Direct Apoptosis & Autophagy

Information and case study data for the PTMScan Direct Apoptosis/Autophagy Service offered by CST.

Stem Cell and Lineage Markers

The ESC and Lineage Markers diagram provides an overview of ESC differentiation along lineage-specific pathways and links to products from CST.

PTMScan® Direct Cell Cycle & DNA Damage

Information and case study data for the PTMScan Direct Cell Cycle and DNA Damage Service offered by CST.

Resources

We observe multiple bands at or around 33-35 kDa in cells with high STING protein expression, such as THP-1 and HDLM-2. Regrettably, we are not certain about what the lower molecular weight band represents in the THP-1 extract. However, customers may be interested in the following possibilities:

1) Please see Wang, P. et al. (2018) Nucleic Acids Res. 46, 4054-4071 (PMID: 29547894; https://pubmed.ncbi.nlm.nih.gov/29547894/). The two bands in THP-1 extract may represent the alpha and beta isoforms of STING.

2) Please see Rodríguez-García, E. et al. (2018) Immunohorizons 2, 363-376 (PMID: 31026807; https://pubmed.ncbi.nlm.nih.gov/31026807/). STING isoforms 2 and 3 do not include #13647’s epitope. However, STING isoform 1 may be detected by #13647.

3) Given that the lower band is also observed in 293T cells transfected with a construct expressing human STING protein (see image on the product webpage; https://www.cellsignal.com//products/primary-antibodies/sting-d2p…

Adaptive Immune Response

Products and Related Resources for Adaptive Immune Response SARS-CoV-2 Research

About Us

Hypoxia and Cancer – the role of HIF-1α in oxygen sensing, metabolism, and tumorigenesis

Molecular oxygen is an essential element for metazoan life. Among its many roles, it functions as the final electron acceptor (oxidizing agent) during oxidative phosphorylation, a metabolic chain-reaction that generates energy in the form of ATP. HIF-1 alpha

Journal Club | Zika virus turns off Akt signaling to hijack autophagy in developing neural tissue

Zika virus turns off Akt signaling to hijack autophagy in developing neural tissue

Resources

Wild type alpha-lytic protease (WaLP) is a serine endopeptidase that cleaves at the carboxyl terminal side of amino acids alanine, serine, threonine, and valine.

WaLP recognizes and cleaves SUMO proteins containing AGG, SGG, TGG or VGG motifs at the SUMO c-terminus.

One should verify that the predicted SUMO sequence from their model organism contains these motifs at the c-terminus.

Resources

Unfortunately, we have not performed experiments in-house to determine if any of our α-Synuclein antibodies can detect oligomers or fibrils of α-Synuclein. We have found a helpful publication in which our α-Synuclein (D37A6) XP^® Rabbit mAb #4179 is used to detect oligomers (PMID: 24898419, Defined α-synuclein prion-like molecular assemblies spreading in cell culture by Aulić, S. et al.).