Resources results for "beta-tubulin antibody"

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Resources (139)

Resources

Based on homology, our β-Tubulin Antibody #2146, β-Tubulin (9F3) Rabbit mAb #2128, β-Tubulin (D3U1W) Mouse mAb #86298, and β-Tubulin (D2N5G) Rabbit mAb #15115 should detect β-Tubulin isoforms A, B, and C. The antigen sequences for these antibodies have 96% homology with beta 2C, beta 4B, beta 5B, and beta 4A isoforms 1, 2, and 3, and 95% homology with beta 2C isoforms A and B. Therefore they are likely to detect these subtypes.

Loading Control Expression in Mouse Tissues

When it comes to mouse tissue samples, not all loading control proteins are expressed equally. Learn which control is right for your research.

Loading Control Antibodies

Ensure equal sample loading with loading control antibodies from CST.

Regulation of Microtubule Dynamics

Expert-reviewed interactive pathway providing a current overview of Regulation of Microtubule Dynamics.

Resources

Based on sequence homology, we predict that our α-Tubulin Antibody #2144 and α-Tubulin (11H10) Rabbit mAb #2125 will detect the alpha 1a, alpha 1b, alpha 1c, alpha 2, alpha 3c, alpha 3d, alpha 3e, alpha 4a, and alpha 4b subtypes.

Neural and Glial Cell Markers

There are a number of markers that can be used to distinguish the many cell types of the central and peripheral nervous system during development, adult neurogenesis, and the pathogenesis of neurodegenerative disorders.

Cytoskeletal Regulation and Vesicle Trafficking

Expert-reviewed interactive signaling pathways providing current overviews of the regulation of actin and microtubule dynamics, as well as links to products from CST.

How do I choose a loading control for my western blot?

Loading controls are essential to ensure data reliability in your western blot. Learn about the three main considerations for loading control selection.

Neuronal, Astrocyte, and Oligodendrocyte Markers

The key to identifying neurons, microglia, oligodendrocytes, and astrocytes lies in using antibodies that target protein biomarkers specifically expressed and localized within these cells.

Organelle Markers

Organelle markers for immunofluorescence analysis from CST Cell Signaling Technology

Antibody Validation for Western Blotting

The accuracy of western blot results relies heavily on the quality of the primary antibody used. CST™ antibodies are validated using multiple experiments.

Resources

Unfortunately, we are not aware if any of our amyloid-beta antibodies will detect the alpha-oligomer of the amyloid-beta protein.

Characterizing the Autophagic-Lysosomal Pathway in Parkinson’s Disease

These antibodies to CNS markers can help study how LRRK2 mutations impact the autophagic-lysosomal pathway (ALP) and contribute to Parkinson's disease.

Amyloid Beta: Amyloid Precursor Protein (APP)

Amyloid beta is a protein fragment of the amyloid precursor protein, and is the major component of Alzheimer’s disease plaques.

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Our Pan-Calcineurin A Antibody #2614 detects endogenous levels of the alpha isoform of Calcineurin A, however it may also recognize the beta and gamma isoforms. Regrettably, because it has been difficult to find suitable models, we have not specifically tested if this product will detect the beta and gamma isoforms. However, the antigenic peptide shares around 90% sequence identity with the beta isoform and around 80% sequence identity with the gamma isoform. Therefore, it is possible that there is cross-reactivity. This antibody does not cross-react with protein phosphatase 1 or 2A.

Resources

It is not uncommon to see a doublet with our beta-Actin antibodies depending on the samples being tested. It is likely caused either by phosphorylation of beta-Actin on its many phosphorylation sites, resulting in a shift in size, or by cross-reactivity with gamma-Actin (beta-Actin = 375 amino acids, gamma-Actin (cytoplasmic form) = 333 amino acids).

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Our Phospho-Acetyl-CoA Carboxylase (Ser79) (D7D11) Rabbit mAbs #11818 and #42801 detect both phospho-ACC-alpha and phospho-ACC-beta. These antibodies recognize ACC-alpha only when phosphorylated at Ser80 in human, which corresponds to Ser79 in mouse and rat. They also recognize ACC-beta only when phosphorylated at Ser222 in human, corresponding to Ser212 in mouse and Ser218 in rat.

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From our testing, we have seen that our Glucocorticoid Receptor (D6H2L) XP® Rabbit mAb #12041 detects both the alpha and beta isoforms of the Glucocorticoid Receptor. It is possible that #12041 may detect other less common isoforms as well, but we have not confirmed this experimentally. Additionally, each of the isoforms that can be detected most likely have post-translational modifications that affect the molecular weight.

Our Glucocorticoid Receptor (D8H2) XP® Rabbit mAb #3660 detects both the alpha and beta isoforms of the Glucocorticoid Receptor. However, based on sequence alignment (and unlike #12041) #3660 is predicted to cross-react with all known alternative translation start site generated isoforms of glucocorticoid receptor-α and glucocorticoid receptor-β (80 kDa).

Please refer to Uniprot ID: P04150 for the reference sequences of both alpha and beta isoforms of the Glucocorticoid Receptor.

Subcellular Marker Selection for Immunofluorescence

There are many reasons to include a subcellular marker in your IF experiment: studying organelle-specific functions, assessing drug effects, and more.

PTMScan® Ser/Thr Kinases

Information and case study data for the PTMScan Direct Ser/Thr Kinases Service, which allows for the targeted screening of a defined set of protein modification sites.

Understanding Neurodegenerative (NDG) Diseases

Neurodegeneration refers to disorders of the CNS that lead to loss of neurons or glia, and is associated with age-related diseases, autoimmune attacks on the nervous system, or genetic mutations that affect the heath of CNS cells.

PTMScan® Direct Apoptosis & Autophagy

Information and case study data for the PTMScan Direct Apoptosis/Autophagy Service offered by CST.

Resources

Our Phospho-Akt (Thr308) (C31E5E) Rabbit mAb #2965 detects over-expressed levels of PKCalpha and beta. It also detects endogenous levels of PKC in Jurkat + CalA extract and possibly weakly in other endogenous models tested. Our Phospho-Akt (Thr308) (L32A4) Mouse mAb #5106 and Phospho-Akt (Thr308) Antibody #9275 detect over-expressed levels of PKCalpha and beta, but do not detect endogenous PKC. Our Phospho-Akt (Thr308) (244F9) Rabbit mAb #4056 detects over-expressed levels of PKCalpha and beta and may also weakly detect endogenous PKC in Jurkat + CalA extract, but not in other endogenous models tested. Our Phospho-Akt (Thr308) (D25E6) XP Rabbit mAb #13038 does not detect over-expressed PKC and is the best choice of the Phospho-Akt (Thr308) antibodies if this is a concern.

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Our Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb #4060 and Phospho-Akt (Ser473) (D9W9U) Mouse mAb #12694 detect over-expressed PKC alpha and beta. Our Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb #4060 also detects endogenous levels of PKC in Jurkat + CalA extract, but not in other endogenous models tested. Our Phospho-Akt (Ser473) (587F11) Mouse mAb #4051 detects over-expressed PKC alpha and beta but does not detect endogenous PKC in the models tested. Our Phospho-Akt (Ser473) (193H12) Rabbit mAb #4058 does not detect over-expressed or endogenous PKC alpha and beta.

Stem Cell and Lineage Markers

The ESC and Lineage Markers diagram provides an overview of ESC differentiation along lineage-specific pathways and links to products from CST.

Resources

We have several antibodies for β-actin that are validated for IHC-P in mouse samples. These are β-Actin (13E5) Rabbit mAb #4970 and β-Actin (8H10D10) Mouse mAb #3700. We would recommend β-Actin (13E5) Rabbit mAb #4970, as β-Actin (8H10D10) Mouse mAb #3700 could be problematic due to mouse on mouse (MOM) background staining.

Resources

PI4 Kinase Antibody #4902 detects endogenous levels of total PI4 Kinase isoform 230. In humans, PI4K230, PI4K92, and PI4K55 are four PI4 kinase isoforms that have been characterized and classified according to their molecular weights of 230, 92, and 55 kDa. In the literature, alpha refers to the 230 kDa size band (UniProt ID: P42356) and beta refers to the 92 kDa size band (UniProt ID:Q9UBF8). Type 2 alpha (UniProt ID: Q9BTU6 ) and type 2 beta (UniProt ID: Q8TCG2) refer to the 55 kDa band.

Cancer Publications from CST

List of publications based on primary research done by the Cancer Research Group at CST (2002-2013).

Acquiring Senescence

Cellular senescence is characterized by irreversible cell-cycle arrest in combination with a distinct secretory phenotype and expanded lysosomes in response to stress. Senescent cells accumulate in tissues during aging, and various markers of senescence

Resources

We believe the 48 kDa band may represent a heterodimer of β-crystallin B1 based on the following references:
https://www.ncbi.nlm.nih.gov/gene/1414
Dolinska MB, Sergeev YV, Chan MP, Palmer I, Wingfield PT. N-terminal extension of beta B1-crystallin: identification of a critical region that modulates protein interaction with beta A3-crystallin. Biochemistry. 2009 Oct 13;48(40):9684-95
Wang KJ, Wang S, Cao NQ, Yan YB, Zhu SQ. A novel mutation in CRYBB1 associated with congenital cataract-microcornea syndrome: the p.Ser129Arg mutation destabilizes the βB1/βA3-crystallin heteromer but not the βB1-crystallin homomer. Hum Mutat. 2011 Mar;32(3):E2050-60