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BrdU Cell Proliferation Protocol: easy to follow directions describing the step by step experimental procedure.
Cell viability assays measure the population of live, viable cells within a sample. Typically, viability assays measure markers of cell health, including cellular metabolism, ATP levels, and cell proliferation.
BrdU Cell Proliferation Chemiluminescent Protocol: easy to follow directions describing the step by step experimental procedure.
In general, the BrdU Cell Proliferation Chemiluminescent Assay Kit #5492 offers a broader dynamic range and higher sensitivity compared to the BrdU Cell Proliferation Assay Kit #6813, which is colorimetric. Both versions should be fully compatible with your needs, however, depending on your plate reader capabilities (absorbance or luminescence), you may want to choose one kit over the other. #6813 is more popular with our customers.
We have not tested changing the final concentration of BrdU while using the BrdU Cell Proliferation Assay Kit. BrdU was originally designed as a chemotherapeutic agent and lethal levels can evoke a DNA damage response, so if the concentration is too high, we are not confident the cells would be healthy enough to proliferate.
Cell states can be monitored using markers correlating to your process of interest. These assays and antibodies can be used to detect cell viability, senescence, proliferation, apoptosis, cytotoxicity and oxidative state.
Cell viability is a measure of the proportion of live, healthy cells within a population. Assays to assess cell viability measure metabolic activity, ATP content, cell proliferation, or membrane integrity.
While performing the BrdU cell proliferation assay, we also notice some changes in cell morphology after adding the fixing/denaturing solution. However, in our experience, it does not adversely affect the signal obtained with the kit. It should be noted that we do not recommend any washes after fixation/denaturation. Instead, we recommend waiting until after incubating the detection solution for one hour at room temperature. No centrifugation step is required unless starting with suspension cell lines.
We recommend that you complete the BrdU cell proliferation assay as soon as you have the time. However, if you are unable to complete the experiment during the recommended time, you can complete the experiment through Step B: BrdU Incorporation and then pause. To do this, please follow the steps below:1. Add the fixing/denaturing solution and incubate for 30 minutes (Part C: BrdU Assay Step 1).2. Remove your solution and dry your plate at room temperature for 30 minutes. 3. Cover plates with parafilm and store at 4C.It is important to make sure your plate is completely dry before you cover with the parafilm and store it. Dry plates can be stored for up to 1 month at 4C, however, the signal of the assay may decrease slightly.
Many cell cycle and proliferation assays exist to measure the amount and rate of cell division. Proliferation can be used as a marker for cell viability.
Flow Cytometry BrdU Modified Protocol: easy to follow directions describing the step by step experimental procedure.
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Immunofluorescence Protocol for Labeling with BrdU Antibody: easy to follow directions describing the step by step experimental procedure.
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