Resources results for "histone h3 antibody"

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Resources (459)

Resources

We see this doublet phenomenon often in a number of different cell lines that we test with our Histone H3 antibodies. It does appear to be sample dependent. The doublet may be due to a proteolytic fragment of Histone H3, either a cleavage product of histone H3 at the N-terminus, or a protein degradation fragment.

Epigenetic Writers and Erasers of Histone H3

Interact with this diagram showing the epigenetic regulators of Histone H3 including those responsible for monomethylation, asymmetric and symmetric dimethylation, and trimethylation.

ChIP-Sequencing performed using the SimpleChIP® Kit

Use SimpleChIP kits to perform high throughput ChIP-Sequencing and assay genome wide changes in histone modifications and transcription factor binding.

Validation for Histone Modification Antibodies

Histone modification antibodies are validated using a peptide array assay at CST

Chromo Protein Domain

A scientific resource for the Chromo protein domain containing information on structure, function, and binding to methylated lysine residues during chromatin remodeling.

Diffuse Intrinsic Pontine Glioma (DIPG)

Discover the most important epigenetic markers that contribute to the progression of diffuse intrinsic pontine glioma (DIPG).

Overview of Cell Proliferation

Cell viability assays measure the population of live, viable cells within a sample. Typically, viability assays measure markers of cell health, including cellular metabolism, ATP levels, and cell proliferation.

Download Pathways by Disease Area

Resources include signaling pathways, antibodies and companion products, research overviews, technical resources, and recent review articles.

Pathways and Diagrams by Disease Area

Find Pathways and Diagrams By Disease Area

Histone Modification Table

The Histone Modification Table provides a referenced list of many known histone modifications, associated modifying enzymes, and proposed functions.

ChIP Validated Antibodies and Primer Pairs

SimpleChIP Kits and Antibodies validated in-house by our antibody development scientists correlated to positive and negative control primers.

Loading Control Expression in Mouse Tissues

When it comes to mouse tissue samples, not all loading control proteins are expressed equally. Learn which control is right for your research.

Hallmarks of Antibody Validation: Complementary Strategies

Hallmarks of Antibody Validation-Complementary Strategies that Employ Western Blot, Immunohistochemistry (IHC), Immunofluorescence (IF/ICC), Flow Cytometry, Histone Antibody, ChIP-qPCR, ChIP-seq.

CUT&Tag Frequently Asked Questions

CUT&Tag Frequently Asked Questions (FAQs) from Cell Signaling Technology, Inc.

Mixed Lineage Leukemia (MLL)

Find out which epigenetic markers are important drivers in mixd lineage leukemia, or MLL.

MBT Protein Repeat

A scientific resource for the MBT protein domain containing information on structure, function, and binding to methyl-lysine motifs during chromatin regulation.

ChIP-seq Ab Performance Comparison

ChIP-seq is a powerful technique that combines ChIP and next-generation sequencing (NGS) to study DNA-protein interactions across the entire genome, but it is only as good as the quality of the antibody used in the ChIP experiment.

Breast Cancer

Discover the key epigenetic drivers of breast cancer and the best products to target them.

CST CUT&RUN Kit: Validated for Flexibility

Maximize your research with our CUT&RUN Kit. Streamline chromatin profiling with our efficient, user-friendly tools designed for deeper, more insightful analyses.

WD40 Protein Domain

A scientific resource for the WD40 protein domain containing information on structure, function, and binding to both phospho-Ser/Thr and methylated lysine residues.

Overview of Chromatin IP Assay Methodology

The chromatin immunoprecipitation (ChIP) assay is a powerful and versatile technique to probe protein-DNA interactions within the natural chromatin context of the cell.

CUT&RUN Frequently Asked Questions

CUT&RUN Frequently Asked Questions (FAQs) from Cell Signaling Technology, Inc.

4 Steps to Better ChIP results - Step 1- Take control

This is a four-part series of tips to improve your ChIP protocol.

Epigenetics

Overview of epigenetic and chromatin regulation pathways, antibodies and related reagents, interactive pathway diagrams, and technical resources for epigenetics research.

CUT&Tag: An Introduction

Learn how CUT&Tag cuts down your chromatin profiling experiment time. You’ll get the same great data with a fraction of the DNA library prep time and improved signal-to-noise.

Hallmarks of Validation - Complementary Strategies

Complementary strategies provide vital information regarding antibody specificity or functionality and can be tailored to the nature of the downstream assay.

Chromatin Immunoprecipitation Technical Support

Detailed technical support information to support ChIP experimental success. LInk to CST's comprehensive ChIP protocol.

How do I choose a loading control for my western blot?

Loading controls are essential to ensure data reliability in your western blot. Learn about the three main considerations for loading control selection.

Protocol Compatibility Table for Immune Signaling and Phenotyping by Flow Cytometry

Use our protocol compatibility table to understand which immune signaling and phenotyping antibodies will work together in your multicolor flow cytometry experiment.

Resources

We do not observe any bias toward euchromatin or heterochromatin in our assays. In CUT&RUN, the target-specific antibody recruits the pAG-MNase and directs digestion to chromatin that is directly adjacent to the antibody, whether that be in euchromatin or heterochromatin. In this case, the active tethering of pAG-MNase to the chromatin allows for digestion to occur even in less accessible heterochromatin. Our CUT&RUN Assay Kit #86652 has been shown to work well with antibodies against active, accessible euchromatic histone modifications (see Tri-Methyl-Histone H3 (Lys4) (C42D8) Rabbit mAb #9751) and activating transcription factors (see NF-kB p65 [D14E12] XP^® Rabbit mAb #8242…