Resources results for "rna modification"
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We see a range of bands between approximately 78 - 82 kDa when using our DRP1 antibodies for western blotting. The bands normally present as a doublet, but sometimes appear as 3 or more bands. We believe that the banding pattern is due to post-translational modification, including phosphorylation, acetylation and sumoylation. Our PhosphoSitePlus page for DRP1 shows some of the known modification sites as well as references (https://www.phosphosite.org/proteinAction.action?id=7003&showAllSites=true).
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ZEB2 mRNA is alternatively spliced and there are post-translational modifications (PTMs), such as ubiquitination and sumoylation, that may be the cause of the larger, 210kDa band. The potential modification sites on the ZEB2 protein can be found at https://www.phosphosite.org/proteinAction.action?id=15497&showAllSites=true. Please also see the UniProt page for human ZEB2 for more sequence and protein information (UniProt: O60315, https://www.uniprot.org/uniprot/O60315).