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Information and case study data for the PTMScan Direct PI3K/Akt Service offered by CST, which allows for targeted screening of 105 proteins within the Akt pathway.
Information and case study data for the PTMScan Direct Tyrosine Kinases Service from Cell Signaling Technology.
The Histone Modification Table provides a referenced list of many known histone modifications, associated modifying enzymes, and proposed functions.
Expert-reviewed interactive pathway providing a current overview of ATP-Dependent Chromatin Remodeling Complexes.
Alphabetical listing of protein, pathway, and antibody acronyms, curated by Cell Signaling Technology.
Information and case study data for the PTMScan Direct Cell Cycle and DNA Damage Service offered by CST.
mIHC with tyramide signal amplification protocol. Detect and localize multiple proteins in tissue sections simultaneously. Click here to learn more.
Find validated CST® matched antibody pairs and streamline the development of your High-Throughput ELISA-Like Immunoassays for screening.
How does the choice of blocking protein—nonfat dry milk vs bovine serum albumen (BSA)—affect your western blot (WB) results? Watch the Tech Tips video.
Loading controls are essential to ensure data reliability in your western blot. Learn about the three main considerations for loading control selection.
When planning your western blot experiment, there are a few things to keep in mind.
The top 5 ways that CST acts to make every day Earth Day.
Happy Thanksgiving from CST.
Companion reagents influence the binding of a primary antibody to its epitope dramatically affecting results. Learn IHC troubleshooting tips in this series
Read about the project to validate CST antibodies for spatial biology research on the Leica Cell DIVE, which can image 60+ biomarkers in one tissue sample.
Do ChIP-validated antibodies work in CUT&RUN assays? Not necessarily. We found only 50-60% of ChIP- or ChIP-seq antibodies were compatible with CUT&RUN.
Chronic neuroinflammation is associated with neurodegenerative diseases like Alzheimer’s, Parkinson’s, amyotrophic lateral sclerosis, and others.
CST antibodies undergo rigorous stability testing to ensure quality. To reduce our carbon footprint, cold shipping is used only when necessary.
A recent publication highlights the advantages and disadvantages of different approaches to multiplex IHC/IF and explains how these methods differ.
SimpleChIP® Plus Sonication Chromatin Immunoprecipitation Protocol (Magnetic Beads): easy to follow directions describing the step by step experimental procedure
Researchers have interrogated BAF complexes for years, but critical roles of chromatin remodelers in cancer and disease have just begun to be elucidated.
Learn DNA library preparation for ChIP-seq and CUT&RUN using the Illumina® protocol, from end repair & adaptor ligation to PCR enrichment & cleanup.
Loading control proteins are often more abundant than the protein of interest, which can lead to the signal exceeding the linear dynamic range of detection
A ranged validation strategy includes both endogenous and heterologous models that express high, moderate, and low levels of the target of interest.
Therapeutic developments in treating Alzheimer’s, novel diagnostic tools, and new insights into neurodegeneration mechanisms were key topics at AD/PD 2023.
3D spheroid models more accurately represent in vivo biological features and are being used in cancer research to mimic 3D tissue organization.
SimpleChIP Plus Chromatin Immunoprecipitation Protocol (Magnetic Beads): easy to follow directions describing the step by step experimental procedure.
The PI3K / Akt Substrates Table provides a comprehensive list of demonstrated downstream targets of Akt phosphorylation.