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Need a conjugated antibody that isn't in the catalog? We can conjugate it for you.

  • Validated, optimized, and tested for stability with the same standards of the conjugated antibodies available in the catalog
  • Save time and avoid the pitfalls associated with do-it-yourself kits
  • Need to validate the conjugated antibody yourself? We also offer faster, lower-cost basic conjugation services

Contact us for more information.

Conjugation Services available:

Conjugation Type Service Tier Service Includes
Alexa Fluor®, Cyanine (CyDye®), DyLight, and Pacific Blue dyes Basic Conjugation + removal of free dye
Tier I Degree of labeling (DOL) + Basic Service
Tier II Validation1 + Tier I Service
Tier III Stability testing + Tier II Service
Phycoerythrin (PE), PE-CyDye® tandems (e.g. PE-Cy®7), and allophycocyanin (APC) Basic Conjugation + purification
Tier I Validation1 + Basic Service
Tier II Stability testing + Tier I Service
Biotin Basic Conjugation + removal of free biotin
Tier I Validation2 + Basic Service
Beads: Sepharose® and Magnetic Basic Conjugation + removal of free antibody
Tier I Validation3 + Basic Service
Horseradish peroxidase (HRP) Basic Conjugation + Purification
Tier I Validation2 + Basic Service

1 Standard validation application method is Flow Cytometry. Alternatives may increase timeline and pricing.
2 Standard validation application method is Western Blot. Alternatives may increase timeline and pricing.
3 Standard validation application method is IP. Alternatives may increase timeline and pricing.

The Alexa Fluor dye conjugates in this product are sold under license from Life Technologies Corporation, for research use only excluding use in combination with DNA microarrays and high content screening (HCS). Alexa Fluor is a registered trademark of Life Technologies, Inc. Cy, Cy7, CyDye, and Sepharose are registered trademarks of GE Healthcare. Pacific Blue is a registered trademark of Molecular Probes, Inc. Dylight is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.
U.S. Patent No. 5,675,063.

Advantages of CST Custom Conjugation

 

  • Flexibility: Tiered options range from basic antibody conjugation to full validation and stability testing of conjugates
  • Validation: Conjugates are tested in key applications using biologically relevant cell systems and controls, and verified for physical integrity using size exclusion chromatography
  • Optimization: Service options include choice of conjugation chemistries, removal of free dye and/or antibody purification, and identification of optimal degree of labeling (DOL) to ensure the best signal-to-noise ratio
  • Support: Application consultation and extensive technical support from the same scientists who produce and validate our commercially available antibody conjugates
 

Application-Validated Antibody Conjugates

The following data illustrate performance of CST conjugated antibodies in a variety of applications. Note that the antibodies shown below are commercially available off-the-shelf; the same team of scientists performs conjugation and validation of both off-the-shelf and custom products.

Flow Cytometry

Flow cytometric analysis of human peripheral blood mononuclear cells

Flow cytometric analysis of human peripheral blood mononuclear cells, untreated (left column) or treated with cross-linked anti-CD3 plus anti-CD28 (10 µg/ml each, 15 min; right column), using Phospho-SLP-76 (Ser376) (D7S1K) XP® Rabbit mAb (PE Conjugate) #16318 (top row) or concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control (PE Conjugate) #5742 (bottom row), and co-stained with an antibody against CD3.

Flow cytometric analysis of PC-3 cells

Flow cytometric analysis of PC-3 cells, untreated (green) or treated with LY294002 #9901 and Wortmannin #9951 (blue), using Phospho-GSK-3Β (Ser9) (D85E12) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate) #14332.

Western Blot

Western blot analysis of NF-κB Control Cell Extracts #9243 from HeLa cells

Western blot analysis of NF-κB Control Cell Extracts #9243 from HeLa cells, untreated (-) or treated with hTNF-α #8902, (20 ng/ml, 5 min., +), using Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb (Biotinylated) #4025.

Immunofluorescence

Confocal immunofluorescent analysis of mouse brain

Confocal immunofluorescent analysis of mouse brain using NeuN (D4G4O) XP® (Alexa Fluor® 488 Conjugate) #54761 (green). Actin filaments have been labeled using DyLight 554 Phalloidin #13054 (red).

Confocal immunofluorescent analysis of mouse primary bone marrow-derived macrophages or BMDM

Confocal immunofluorescent analysis of mouse primary bone marrow-derived macrophages (BMDMs) differentiated with mM-CSF (20 ng/mL, 8 days), and then either treated with LPS (50 ng/mL, overnight; left), or LPS (50 ng/mL, overnight) followed by Nigericin (10 uM, 2 hr; right), using ASC (D2W8U) Rabbit mAb (Mouse Specific) (Alexa® 488 Conjugate) #17507 (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). Note the translocation of ASC to inflammasomes following stimulation with LPS and Nigericin (white arrows).