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Western Blot Resources

Western Blotting team

We’ve compiled our most useful western blot resources to help with your next experiment. These videos and guides will help get you started, but remember to reach out to our technical services team if you still have trouble getting clear results.

Everything You Need for a Successful Western Blot:

Western Blotting

This clickable, printable list of products and procedures will help make your next western a success.

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Companion Products

Western Blotting

For a listing of Western Blot loading controls, antibodies, reagents, and buffers, view page 2-3 of this companion products brochure.

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Protocol

Protocol

Step through our western blot protocol so you can replicate the procedure and achieve reliable, reproducible results.

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Choosing Positive Controls

Choosing Positive Controls

Not all positive controls are created equal. Let’s find the right one for your experiment.

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Milk or BSA for Western Blot?

Milk or BSA for Western Blot?

How does your choice of blocking protein—nonfat dry milk vs bovine serum albumin (BSA)—affect your western blot results?

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Choosing a Lysis Buffer

Choosing a Lysis Buffer

Need to decide between different lysis buffers for your western blot? Here are some questions to consider.

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Sonicating Your Lysis Buffer

Sonicating Your Lysis Buffer

Do you sonicate your samples before running your western blots? If not, you might want to check out this video.

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Troubleshooting

Troubleshooting

Tips to help you diagnose problems and identify solutions to ensure you get the expected results in the shortest amount of time.

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Western Blot Application Guide

Western Blotting Guide

This application guide includes protocols, troubleshooting tips, and data illustrating critical factors for western blotting success.

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Need help choosing the right western blot products for your experiment? Contact Us.