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Experimental Design: Analyzing Intracellular Signaling With Flow Cytometry

 

Flow cytometry can be leveraged to measure intracellular signaling states using antibodies specific for post-translational modifications, including phosphorylation. You can design experiments that incorporate inhibitors, agonists, or antagonists to modulate signaling activities in your samples and test hypotheses about the signaling mechanisms that may play a role in your model system. In this video, Rob and Sarah present five tips for successful intracellular flow experiments.