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How Does Antibody Diluent Affect Staining in Immunohistochemistry (IHC)?

 

Learn how choice of antibody diluent can affect staining results in immunohistochemistry (IHC). There are several steps in the IHC protocol that can impact your results. In this video, we're going to focus on antibody diluent, which is the solution that primary antibody is diluted into for incubation with a tissue sample. It's important to use an antibody diluent that works with your particular antibody, as the choice of diluent can impact the standing results. The optimal antibody diluent supports antibody-epitope interactions and specificity, and ultimately, the strength of your staining.