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How to Combine Surface & Intracellular Targets in Flow Cytometry

 

Multiplex flow cytometry experiments that combine antibodies for surface markers with intracellular targets allow you to characterize signaling activities in distinct cellular populations. Finding a multiplex flow protocol that is compatible with antibodies for both surface and intracellular proteins may require some testing and optimization. In this Tech Tip, Rob introduces approaches to setting up a flow protocol based on what is known about your antibody’s compatibility with fixation and permeabilization. More flow cytometry videos and resources: