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Learning Lab LIVE! Imaging Q&A with IF expert Virginia Bain, PhD

 

The results of your immunofluorescence (IF) experiment are impacted by several factors, including which type of microscope and imaging system you choose. In this edition of Learning Lab LIVE!, IF Expert Virginia Bain, PhD, discusses what you should keep in mind when starting an IF experiment, including:

  • Capabilities and limitations of different types of microscopes and imaging equipment;
  • Magnification considerations;
  • Widefield epifluorescence basics and imaging settings;
  • Confocal microscopy basics and imaging settings;
  • Section thickness and when to use z-stacks;
  • Critical importance of reagent validation and sample quality.

Download the comprehensive Guide to Successful Immunofluorescence from our library of free application resources.

Watch the previous session on Fluorophore Considerations, plus more videos for other applications on the Learning Lab LIVE! channel page.