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Custom Conjugation Services

Need a conjugated antibody that isn't in the catalog? We can conjugate it for you.

  • Validated, optimized, and tested for stability with the same standards of the conjugated antibodies available in the catalog
  • Save time and avoid the pitfalls associated with do-it-yourself kits
  • Need to validate the conjugated antibody yourself? We also offer faster, lower-cost basic conjugation services

Contact us for more information.

Conjugation Services available:

Conjugation Type Service Tier Service Includes
Alexa Fluor®, Cyanine (CyDye®), DyLight, and Pacific Blue dyes Basic Conjugation + removal of free dye
Tier I Degree of labeling (DOL) + Basic Service
Tier II Validation1 + Tier I Service
Tier III Stability testing + Tier II Service
Phycoerythrin (PE), PE-CyDye® tandems (e.g. PE-Cy®7), and allophycocyanin (APC) Basic Conjugation + purification
Tier I Validation1 + Basic Service
Tier II Stability testing + Tier I Service
Biotin Basic Conjugation + removal of free biotin
Tier I Validation2 + Basic Service
Beads: Sepharose® and Magnetic Basic Conjugation + removal of free antibody
Tier I Validation3 + Basic Service
Horseradish peroxidase (HRP) Basic Conjugation + Purification
Tier I Validation2 + Basic Service
  • Flexibility: Tiered options range from basic antibody conjugation to full validation and stability testing of conjugates
  • Validation: Conjugates are tested in key applications using biologically relevant cell systems and controls, and verified for physical integrity using size exclusion chromatography
  • Optimization: Service options include choice of conjugation chemistries, removal of free dye and/or antibody purification, and identification of optimal degree of labeling (DOL) to ensure the best signal-to-noise ratio
  • Support: Application consultation and extensive technical support from the same scientists who produce and validate our commercially available antibody conjugates

The following data illustrate performance of CST conjugated antibodies in a variety of applications. Note that the antibodies shown below are commercially available off-the-shelf; the same team of scientists performs conjugation and validation of both off-the-shelf and custom products.

Flow Cytometry

Flow cytometric analysis of human peripheral blood mononuclear cellsFlow cytometric analysis of human peripheral blood mononuclear cells, untreated (left column) or treated with cross-linked anti-CD3 plus anti-CD28 (10 µg/ml each, 15 min; right column), using Phospho-SLP-76 (Ser376) (D7S1K) XP® Rabbit mAb (PE Conjugate) #16318 (top row) or concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control (PE Conjugate) #5742 (bottom row), and co-stained with an antibody against CD3.

 

Flow cytometric analysis of PC-3 cells

Flow cytometric analysis of PC-3 cells, untreated (green) or treated with LY294002 #9901 and Wortmannin #9951 (blue), using Phospho-GSK-3Β (Ser9) (D85E12) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate) #14332.

Western Blot

Western blot analysis of NF-κB Control Cell Extracts #9243 from HeLa cells

Western blot analysis of NF-κB Control Cell Extracts #9243 from HeLa cells, untreated (-) or treated with hTNF-α #8902, (20 ng/ml, 5 min., +), using Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb (Biotinylated) #4025.

Immunofluorescence

Confocal immunofluorescent analysis of mouse brain

Confocal immunofluorescent analysis of mouse brain using NeuN (D4G4O) XP® (Alexa Fluor® 488 Conjugate) #54761 (green). Actin filaments have been labeled using DyLight 554 Phalloidin #13054 (red).

Confocal immunofluorescent analysis of mouse primary bone marrow-derived macrophages or BMDM

Confocal immunofluorescent analysis of mouse primary bone marrow-derived macrophages (BMDMs) differentiated with mM-CSF (20 ng/mL, 8 days), and then either treated with LPS (50 ng/mL, overnight; left), or LPS (50 ng/mL, overnight) followed by Nigericin (10 uM, 2 hr; right), using ASC (D2W8U) Rabbit mAb (Mouse Specific) (Alexa® 488 Conjugate) #17507 (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). Note the translocation of ASC to inflammasomes following stimulation with LPS and Nigericin (white arrows).