Technical support
Results (637)
Why have the names of your phospho-Smad1/5/8 antibodies changed to phospho-Smad1/5/9?
Smad8 and Smad9 are often used in the literature to refer to the same protein. However, the official name of the protein is Smad9. Moreover, there is a Smad8 in Xenopus that is not homologous. Therefo...
How can I increase the western blot signal for ubiquitin?
The ubiquitin signal can be increased by using a semi-dry-transfer and autoclaving the membrane for 30 minutes on a wet cycle or boiling the membrane for 30 minutes after transfer.
Will the β-Actin Antibody #4970 detect both full-length β-actin as well as the cleaved fragments?
β-actin can be cleaved by caspase-3 between Asp244 and Gly245, which will result in a 32kDa fragment and a 15kDa fragment. Due to the location of its antigen, our β-Actin Antibody #4970 will detect bo...
Can you provide the identity of the capture and detection antibodies in your PathScan® ELISA kits?
Unfortunately, because our ELISA antibody pairs are extensively optimized for performance, this information is proprietary. If you are interested in optimizing your own antibody pair, we offer the fol...
How should I prepare my tissue extracts for western blot?
Our tissue extracts are prepared as follows:
- Cut a small piece of tissue (approximately 3 mm^3).
- Place in a clean Dounce homogenizer.
- Add 1mL cold 1X
Why do you recommend optimal antibody dilutions for ChIP, ChIP-seq and CUT&RUN?
In ChIP, ChIP-seq, and CUT&RUN, the performance of an antibody depends on its sensitivity and activity. Antibody performance does not always increase with concentration; sometimes an antibody perf...
What is the band at 90 kDa detected by the TrkA Antibody #2505?
TrkA is a highly glycosylated protein. Therefore, its size can differ depending on the amount of glycosylation present. Un-glycosylated TrkA is 87 KDa, so this is likely what the 90kDa band is on th...
What is the cross-reactivity of the Claudin-6 (E2S5M) Rabbit mAb #62831 with other Claudin family proteins?
The peptide sequence used to produce our Claudin-6 (E2S5M) Rabbit mAb #62831 is located near the carboxy terminus of human Claudin-6 and was carefully selected to avoid cross-reaction with other Claud...
What is the 140 kDa band detected by the ATP2A2/SERCA2 Antibody #4388?
The 140 kDa band detected by our ATP2A2/SERCA2 Antibody #4388 appears to be consistent with SUMOylation based on Kho, C et al. (2011) Nature 477, 601-5 (PMID: 21900893, https://www.ncbi.nlm.nih.gov/pu...
Which of your phospho-GSK-3β antibodies will detect the GSK-3β-Tag?
Phospho-GSK-3β antibodies that detect phosphorylation at Ser9 upon activation in properly treated samples are also capable of detecting the GSK-3β-Tag. Although these antibodies are specific to the...
Does your CaMKII (pan) (D11A10) Rabbit mAb #4436 detect the delta isoform?
Yes, our CaMKII (pan) (D11A10) Rabbit mAb #4436 detects the delta isoform.
Can I combine antibodies used in a SignalStar™ assay with direct conjugates?
SignalStar Multiplex IHC kits and reagents have been validated for use in combination with direct conjugates. The SignalStar™ Fluorescence Removal Kit
Why do the VASP antibodies detect a doublet?
The 48 kDa band represents VASP phosphorylated at Ser239 and the 50 kDa band represents VASP phosphorylated at both Ser239 and Ser157. The hyper-phosphorylation that occurs when both serines are phosp...
Are the light chains of the β-Actin (8H10D10) Mouse mAb #3700 kappa or lambda?
The β-Actin (8H10D10) Mouse mAb #3700 has kappa light chains.
Will the APOBEC3G (D9C6Z) Rabbit mAb #43584 detect other APOBEC family members?
Our APOBEC3G (D9C6Z) Rabbit mAb #43584 was produced with an antigenic peptide corresponding to residues surrounding Gly255 of the human APOBEC3G protein (see Uniprot ID #Q9HC16; https://www.uniprot.or...
How much of each antibody is supplied in the StemLight™ Pluripotency Antibody Kit #9656 and how does this relate to the number of tests?
There is 50 µl of each antibody component that is listed in the StemLight Pluripotency Antibody Kit #9656. The kit components are pre-optimized for parallel use in immunofluorescent analysis. There ar...
Why do your DRP1 antibodies detect a doublet by western blot?
We see a range of bands between approximately 78 - 82 kDa when using our DRP1 antibodies for western blotting. The bands normally present as a doublet, but sometimes appear as 3 or more bands. We beli...
How do I contact the CST Customer Service team?
Our customer service team is available Monday through Friday from 8:30 AM to 8 PM ET. You can reach the team by email at [email protected] or by phone at 877-616-2355.
Why are your PBS-formulated antibodies pH 8.0, not pH7.5 or 7.2?
By using a pH that is a bit more basic and above the isoelectric point of a typical IgG, we can minimize antibody aggregation. This formulation is also conjugation-friendly.
Do you have mouse-reactive antibodies available for SignalStar™ Multiplex IHC?
Yes, please select “Mouse” in the first step of the SignalStar Multiplex IHC Panel Builder ...
Can the Senescence β-Galactosidase Staining Kit #9860 be used with suspension cells?
We have only tested our Senescence β-Galactosidase Staining Kit #9860 with adherent cells. However, we commonly recommend immobilizing suspension cells to a glass slide by using a cytospin. Once the c...
When comparing my SignalStar™ staining to the chromogenic staining on a serial section, I see more positive cells. How do I know if this excess staining is correct?
During the course of optimization, we’ve found that fluorescent staining may show higher %-positivity than chromogenic staining. To ensure any excess staining is specific, confirm that the correct sub...
What is the best way to choose an antibody for my CUT&Tag assay if I can not find a CUT&Tag-validated antibody for my target protein?
We always recommend starting with CST CUT&Tag-validated antibodies because CST scientists perform intensive in-house tests to exclude any antibody that tends to show non-specific tagmentation at o...
How did you determine that a NG-seq sequencing depth of 3 to 5 million sequencing reads was sufficient for CUT&RUN analysis?
This decision was based on our analysis of hundreds of CUT&RUN samples. We found that a sequencing depth lower than 3 million generated too few peaks and that the number of peaks increased with de...
Which LC3 antibody should I choose for my samples?
LC3 exists as three highly homologous isoforms or paralogs. These are LC3A, LC3B, and LC3C. These LC3 paralogs have different patterns of expression in human tissues [see He, H et al. (2003) J Biol Ch...
Which total Akt antibody do you suggest for paraffin-embedded immunohistochemistry (IHC-P) with human samples?
We have several monoclonal antibodies for detecting total Akt protein that are validated for IHC-P in human samples. These are as follows:
- Akt (pan) (C67E7) Rabbit mAb #4691
- Akt (pan)...
Can I use fractionated samples from the Cell Fractionation Kit #9038 for immunoprecipitation?
The buffers provided in the Cell Fractionation Kit #9038 (as well as the final working stock solutions) contain SDS Therefore, it may be necessary to purify the fractionated samples using a commercial...
Can I use PTMScan® LysC Protease #84748 for tandem mass tag (TMT) labeling?
We have used LysC in conjunction with trypsin for TMT-labeling-based studies to reduce miscleavages. For TMT-based workflows, we recommend a higher LysC:substrate ratio of 1:100 compared to the 1:250 ...
Must I perform an Fc receptor blocking step with the CD16/CD32 (2.4G2) Rat mAb #88280 in every immunostaining experiment?
It is difficult to predict when the Fc receptor blocking step is necessary, due to the variable interaction of host species with different antibody isotypes and the interference caused by fluorescent ...
Do I need to perform Fc blocking when using rabbit or mouse antibodies in flow cytometry?
Human Fc-gamma Receptor 1a (FCGR1A) exhibits strong cross-reactivity with rabbit IgG, and mouse IgG2a, IgG2c, and IgG3. Of course, mouse Fc-gamma Receptors will react with mouse antibodies. In flow cy...