Technical Support Articles
Results (690)
How should I resuspend and aliquot the PTMScan® LysC Protease #84748?
To resuspend the PTMScan® LysC Protease #84748, we recommend allowing the vial to equilibrate at room temperature for a few minutes after removing from the -20C freezer. Next, spin the vial at 1,0...
How does the specificity of the F(ab’)2 fragments differ from the corresponding full-length monoclonal antibodies?
What is the solution formulation of the PTMScan Control peptides?
The solution formulation of the PTMScan Control peptides is 25% acetonitrile, 74.9% water, 0.1% trifluoroacetic acid (V:V).
Why is the concentration of my antibody so low?
At CST, our antibodies are formulated on the basis of their reactivity and performance for each approved application at a recommended dilution. For antibodies with high affinity, optimal performan...
Which cleaved caspase-3 (Asp175) antibody do you suggest for immunofluorescent immunocytochemistry (IF-IC) with human samples?
We have several antibodies for detecting Cleaved Caspase-3 (Asp175) that are validated for IF-IC with human samples. These are:
- Cleaved Caspase-3 (Asp175) Antibody #9661
- Cleaved Caspas...
Does your CUT&RUN Assay Kit work with isolated nuclei?
Our CUT&RUN Assay Kit is optimized for use with whole cells and works with a large number of cell lines, both adherent and suspension. While it is not typically necessary to pre-isolate cell nucle...
Should digitonin be included in my wash buffers for my entire CUT&Tag experiment?
Yes, digitonin needs to be present in all wash buffers during the entire CUT&Tag experiment. This is because cell membrane permeabilization caused by digitonin is reversible. Removing or lowering ...
Should I be concerned that my Concanavalin A beads "clump together" in some of my CUT&RUN experiments?
The Concanavalin A beads can clump together rather easily, especially when the cells become lysed. To avoid this, make sure your cells are healthy and be sure to treat them very gently during the cell...
Why can't I add a target to the channel I want when using the SignalStar™ Multiplex IHC Panel Builder?
During our extensive validation process, we found that some targets do not perform optimally with specific fluorophore pairings. When this is the case, the SignalStar Multiplex IHC Panel Builder will ...
Why do I need to use the three buffers in the Cell Fractionation Kit #9038?
Each buffer in the Cell Fractionation Kit #9038 contains detergents to help isolate different cellular fractions. The Cytoplasmic Isolation Buffer (CIB) contains a non-ionic detergent used as a cell p...
Do you have human-reactive antibodies available for SignalStar™ Multiplex IHC?
Yes, please select “Human” in the first step of the SignalStar Multiplex IHC Panel Builder ...
Can you provide data that confirms the specificity of your Raptor (24C12) Rabbit mAb #2280?
The specificity of our Raptor (24C12) Rabbit mAb #2280 has been confirmed by siRNA in the following papers:
PMID: 28648777 (https://pubmed.ncbi.nlm.nih.gov/28648777/) Figure 2B
PMID: 249...
Can I use primers from the Illumina Nextera Library preparation system or design my own primers to amplify the DNA library generated from the CUT&Tag Assay Kit #77552??
Yes, you can use primers from the Illumina Nextera Library preparation system or self-designed primers to amplify the DNA library generated from the CUT&Tag DNA using the CUT&Tag Assay Kit #77...
How stable is the Senescence β-Galactosidase Staining Kit #9860 after multiple freeze/thaws?
We are confident that the Senescence β-Galactosidase Staining Kit #9860 will continue to perform as expected up to at least 3 freeze/thaw cycles. We recommend aliquoting your reagents into single use ...
Do I need to optimize the SignalStar™ Multiplex IHC Kits & Reagents for the type of tissue I’m using?
The SignalStar Multiplex IHC kits and reagents have been optimized with respect to fluorophore pairing and order of antibodies. As tissues vary in quality and expression level of biomarkers, increasin...
Can I reuse PTMScan® antibody beads?
The “classic” PTMScan® kits that use agarose beads and the PTMScan HS kits that use magnetic beads are both designed for a single PTM peptide enrichment. The nature of the acidic elution step, which l...
How are the SignalStar™ Multiplex IHC Kits & Reagents validated?
CST thoroughly validates each antibody available in the CST antibodies undergo thorough validation processes to ensure reliable performance for every approved application. This includes testing different protocol variations to identify the most effective o... We have several antibodies for detecting β-actin that are validated for IF-IC with human samples. These include: Can I modify the CST-recommended protocol for a given application?
Which β-actin antibody do you suggest for immunofluorescence-immunocytochemistry (IF-IC) in human samples?