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Render Timestamp: 2025-01-17T01:42:54.600Z
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Technical support

Results (655)

My items say to store at -20°c but they were shipped in an envelope. Is it ok to use?

Yes, it is safe to use these items. Since 2003, CST has been shipping antibodies at room temperature when possible to reduce waste from packing materials. All CST antibodies are stability tested by ex...

Can I autoclave the 6-Tube Magnetic Separation Rack #7017?

No, these racks cannot be autoclaved. Exposing them to high autoclave temperatures may cause the magnets to lose their magnetism. Instead, we suggest submerging or spraying the rack with 10% bleach...

What is your protocol for PNGase F treatment?

PNGase F cell treatment:
To deglycosylate a protein of interest in native conditions.

Reagents: 
PNGase F, NEB P0704L (https://...

Can the Senescence β-Galactosidase Staining Kit #9860 be used with frozen tissue?

While we have not tested the Senescence β-Galactosidase Staining Kit #9860 with frozen tissue in-house, we have spoken with numerous customers who have done so with success using our recommended cell ...

What is an appropriate positive control to include in this SignalStar™ assay? Are multiple controls necessary?

Any tissue shown to be positive for each marker via chromogenic IHC can serve as a positive control tissue for a SignalStar™ assay. Each target will therefore require a positive control, which may som...

Do you have mouse-reactive antibodies available for SignalStar™ Multiplex IHC?

Yes, please select “Mouse” in the first step of the SignalStar Multiplex IHC Panel Builder ...

When comparing my SignalStar™ staining to the chromogenic staining on a serial section, I see more positive cells. How do I know if this excess staining is correct?

During the course of optimization, we’ve found that fluorescent staining may show higher %-positivity than chromogenic staining. To ensure any excess staining is specific, confirm that the correct sub...

What is the best way to choose an antibody for my CUT&RUN assay if I cannot find a CUT&RUN validated antibody for my target protein?

When selecting an antibody for use in CUT&RUN, we recommend starting with a ChIP or ChIP-seq-validated antibody; however, we have found that not all ChIP-validated antibodies work in the CUT&am...

What is the extinction coefficient of my rabbit antibody?

The molar extinction coefficient of rabbit IgG at 280 nm is approximately 210,000 M-1cm-1.

How long after the completion of staining can I wait to image my slides when performing a SignalStar™ Multiplex IHC assay?

For Imaging Round 1, the staining should show robust signal when imaged up to 8 hours post completion of staining. For Imaging Round 2, imaging should be performed as close to the completion of staini...

Can I combine antibodies used in a SignalStar™ assay with direct conjugates?

SignalStar Multiplex IHC kits and reagents have been validated for use in combination with direct conjugates. The SignalStar™ Fluorescence Removal Kit 

Can my slides be used for other assays after SignalStar™ Multiplex IHC?

SignalStar™ Multiplex IHC Kits & Reagents have not been validated for use in combination with other assays. The SignalStar technology does not destroy the tissue, so it may be possible to perform ...

Which phospho-Akt (Ser473) antibody do you suggest for Immunofluorescence (Immunocytochemistry) (IF-IC) with human samples?

We have several antibodies for detecting the phosphorylation of Akt at Ser 473 that are validated for IF-IC with human samples. These include the following: