Technical support
Results (655)
What is the species reactivity of the SUMO remnant PTMScan® kits that utilize WaLP for peptide digest?
Wild type alpha-lytic protease (WaLP) is a serine endopeptidase that cleaves at the carboxyl terminal side of amino acids alanine, serine, threonine, and valine.
WaLP recognizes and cleaves SUM...
Will the RAG1 (D36B3) Rabbit mAb #3968 cross-react with human RAG2?
Our RAG1 (D36B3) Rabbit mAb #3968 was produced using a recombinant protein fragment with a sequence toward the N-terminal region of human RAG1. No significant homology is found in this region when ali...
Do you have any tips for using FACS-isolated cells in CUT&RUN experiments?
We have no specific tips for using FACS-isolated cells in CUT&RUN experiments. Like a cultured cell suspension, if your isolated cells are in less than 40ul PBS, you can move directly to Concanava...
Are your siRNAs pools or single duplexes?
All of our siRNAs are single duplexes. We discontinued pools a number of years ago to avoid off-target effects.
Are there any lot-to-lot differences when using your Vimentin (V9) Mouse mAb #49636 for western blot?
There may be some lot-to-lot variability in the signal strength of our Vimentin (V9) Mouse mAb #49636 when testing by western blot due to this antibody being sold by weight instead of reactivity.
Can you provide data that confirms the specificity of your Raptor (24C12) Rabbit mAb #2280?
The specificity of our Raptor (24C12) Rabbit mAb #2280 has been confirmed by siRNA in the following papers:
PMID: 28648777 (https://pubmed.ncbi.nlm.nih.gov/28648777/) Figure 2B
PMID: 249...
Why do I need to use the three buffers in the Cell Fractionation Kit #9038?
Each buffer in the Cell Fractionation Kit #9038 contains detergents to help isolate different cellular fractions. The Cytoplasmic Isolation Buffer (CIB) contains a non-ionic detergent used as a cell p...
How are the SignalStar™ Multiplex IHC Kits & Reagents validated?
CST thoroughly validates each antibody available in the Yes, you can use primers from the Illumina Nextera Library preparation system or self-designed primers to amplify the DNA library generated from the CUT&Tag DNA using the CUT&Tag Assay Kit #77... For CUT&Tag assays using adherent cell lines, the first step is to detach the cells from the dish. We recommend using trypsin to detach the cells. Alternatively, Accutase Cell Dissociation Reagent... CST antibodies undergo thorough validation processes to ensure reliable performance for every approved application. This includes testing different protocol variations to identify the most effective o... To avoid intra-sample batch effects during liquid chromatography with tandem mass spectrometry (LC-MS/MS), we recommend a run order that involves running one technical replicate of each enrichment, th... To resuspend the PTMScan® LysC Protease #84748, we recommend allowing the vial to equilibrate at room temperature for a few minutes after removing from the -20C freezer. Next, spin the vial at 1,0... It has been determined that our Anti-rabbit IgG, HRP-linked Antibody #7074 has the following low cross-reactivities with species other than rabbit:
Can I use primers from the Illumina Nextera Library preparation system or design my own primers to amplify the DNA library generated from the CUT&Tag Assay Kit #77552??
What advice do you have for collecting samples of adherent cell lines versus suspension cell lines for CUT&Tag experiments?
Can I modify the CST-recommended protocol for a given application?
How do I avoid batch effects and what sample run order should I use for PTMScan® experiments?
How should I resuspend and aliquot the PTMScan® LysC Protease #84748?
What is the species cross-reactivity of your Anti-rabbit IgG, HRP-linked Antibody #7074?
Do you have human-reactive antibodies available for SignalStar™ Multiplex IHC?
Yes, please select “Human” in the first step of the SignalStar Multiplex IHC Panel Builder ...
How do you make the 4X RNA Denaturing Buffer for RNA dot blot?
4X RNA Denaturing Buffer Recipe for use with our RNA dot blot protocol:
657ul Formamide @99.5%
210ul of 37% Formaldehyde
133ul of 10x MOPS buffer
Final formulation (considering rou...
Do I need to optimize the SignalStar™ Multiplex IHC Kits & Reagents for the type of tissue I’m using?
The SignalStar Multiplex IHC kits and reagents have been optimized with respect to fluorophore pairing and order of antibodies. As tissues vary in quality and expression level of biomarkers, increasin...
What is the cross-reactivity of your Myosin Light Chain 2 antibodies with Myosin 12a/b?
We have not tested the reactivity of our myosin light chain 2 antibodies against myosin 12a/b (UniProt: P19105 and O14950). However, based on homology, there is a good chance for cross-reactivity. The...
Where can I find the RRID numbers for CST antibodies?
RRIDs (Research Resource Identifiers) can be found in the RRID Portal (https://scicrunch.org/resources). Simply search for the product number, clone number, or target along with 'Cell Signaling T...
When comparing my SignalStar™ staining to the chromogenic staining on a serial section, I see more positive cells. How do I know if this excess staining is correct?
During the course of optimization, we’ve found that fluorescent staining may show higher %-positivity than chromogenic staining. To ensure any excess staining is specific, confirm that the correct sub...
Which β-actin antibody do you suggest for immunofluorescence-immunocytochemistry (IF-IC) in human samples?
We have several antibodies for detecting β-actin that are validated for IF-IC with human samples. These include:
- β-Actin (13E5) Rabbit mAb #4970
- β-Actin (D6A8) Rabbit mAb #...
Can I combine antibodies used in a SignalStar™ assay with direct conjugates?
SignalStar Multiplex IHC kits and reagents have been validated for use in combination with direct conjugates. The SignalStar™ Fluorescence Removal Kit For custom (carrier-free) orders, many antibodies are available pre-formulated in PBS without BSA or glycerol. This carrier-free formulation is amenable to conjugation and can be conjugated to fluorop... We have two antibodies for detecting caspase-3 that are validated for IHC-P with human samples, Caspase-3 Antibody #9662 and Caspase-3 (D3R6Y) Rabbit mAb (IHC Formulated) #14214. We recommend #14214 f... The VersicanG1-Fc (Rabbit IgG1) #15933 contained in the Hyaluronan Complete Tissue Staining Kit (HRP) #38822 and Hyaluronan Complete Tissue Staining Kit (Alexa Fluor® 488) #53721 has been tested succe... CST scientists test all our antibodies in relevant applications such as western blotting, immunoprecipitation, immunofluorescence (ICC), immunohistochemistry, flow cytometry, ELISA, and chromatin immu... We have several antibodies for detecting caspase-3 when cleaved at Asp175 that are validated for IHC-P with human samples. These are:
Can I order a custom formulation of an antibody?
Which caspase-3 antibody do you suggest for paraffin-embedded immunohistochemistry (IHC-P) with human samples?
Is the VersicanG1-Fc (Rabbit IgG1) #15933 product homologous with all species?
How does CST ensure the quality of their antibodies?
Which cleaved caspase-3 (Asp175) antibody do you suggest for paraffin-embedded immunohistochemistry (IHC-P) with human samples?
How much CUT&RUN DNA is required for NG-seq analysis?
The amount of CUT&RUN DNA required for NG-seq analysis depends on the sensitivity of the DNA library prep kit. The typical yield for CUT&RUN DNA is 0.6 to 6 ng per reaction. Our The “classic” PTMScan® kits that use agarose beads and the PTMScan HS kits that use magnetic beads are both designed for a single PTM peptide enrichment. The nature of the acidic elution step, which l... Our CUT&RUN Assay Kit #86652 is compatible with downstream qPCR analysis. The pr...Can I reuse PTMScan® antibody beads?
Can CUT&RUN enriched DNA be analyzed by qPCR, or do I have to do NGseq to analyze my data?