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Results (695)

What PD-L2 antibodies do you currently offer?

We currently offer three products targeting PD-L2:

Our PD-L2 (D7U8C) XP® Rabbit mAb #82723 recognizes endogenous levels of total human PD-L2 protein and was produced by immunizing animals with ...

Why are there particulates in my sample of Animal-Free Blocking Solution (5X) #15019?

Particulates may occasionally be seen in our Animal-Free Blocking Solution (5X) #15019 due to material becoming caught in the threads of the cap/bottle and drying out over a short period of time. Thes...

Can the SARS-CoV-2 serological ELISA kits assess the effectiveness of a SARS-CoV-2 vaccine using vaccine-injected human blood samples?

Yes, potentially all of our SARS-CoV-2 serological ELISA kits could be useful for studying samples from vaccine-injected subjects. Please follow

How long after the completion of staining can I wait to image my slides when performing a SignalStar™ Multiplex IHC assay?

For Imaging Round 1, the staining should show robust signal when imaged up to 8 hours post completion of staining. For Imaging Round 2, imaging should be performed as close to the completion of staini...

Can you provide data that confirms the specificity of your Raptor (24C12) Rabbit mAb #2280?

The specificity of our Raptor (24C12) Rabbit mAb #2280 has been confirmed by siRNA in the following papers:

PMID: 28648777 (https://pubmed.ncbi.nlm.nih.gov/28648777/) Figure 2B

PMID: 249...

I don’t see my target of interest in your menu of available antibodies for SignalStar™ Multiplex IHC. Can I still use it in my panel in some way?

SignalStar Multiplex IHC kits and reagents haven’t yet been validated for use with antibodies outside of our menu. We’re in the process of developing cust...

What is the extinction coefficient of my rabbit antibody?

The molar extinction coefficient of rabbit IgG at 280 nm is approximately 210,000 M-1cm-1.

In terms of cytokine activity, what is the difference between International Units (IU) and specific activity?

International Units (IU) and specific activity are two different ways of measuring...

What are the methods for PTMScan® mass spectrometry?

The methods for the LC and MS are shown below:
 

 <...

Does your CUT&RUN Assay Kit work with isolated nuclei?

Our CUT&RUN Assay Kit is optimized for use with whole cells and works with a large number of cell lines, both adherent and suspension. While it is not typically necessary to pre-isolate cell nucle...

What is the expected size distribution of CUT&RUN DNA?

When using antibodies for CUT&RUN against histone modifications, we typically see fragment sizes as small as 150 bp (size of a mono-nucleosome) and quite often see a nucleosomal ladder of DNA frag...

Can I use your RIPA Buffer (10X) #9806 for Native PAGE?

We do not recommend our RIPA Buffer (10X) #9806 for Native PAGE as it is too stringent. Unfortunately we do not carry a buffer appropriate for Native PAGE.

Can CUT&RUN enriched DNA be analyzed by qPCR, or do I have to do NGseq to analyze my data?

Our CUT&RUN Assay Kit #86652 is compatible with downstream qPCR analysis. The pr...

Which total Akt antibody do you suggest for immunofluorescent immunocytochemistry (IF-IC) with human samples?

We have several antibodies for detecting total Akt protein that are validated for IF-IC with human samples. These are as follows:

  • Akt (pan) (C67E7) Rabbit mAb #4691
  • Akt (pan) (11E7) Ra...

    What DNA library prep kit do you recommend using for CUT&RUN?

    We recommend using our SimpleChIP® ChIP-seq DNA Library Prep Kit for Illumina® #567...

    How can I confirm the PTMScan® LysC Protease #84748 digested my proteins efficiently?

    To confirm digestion, we recommend taking a ~1% aliquot of the protein sample pre- and post-digestion and running them on an SDS-PAGE gel with Coomassie blue staining. After digestion, you should ...

    Which total Akt antibody do you suggest for paraffin-embedded immunohistochemistry (IHC-P) with mouse samples?

    We have several monoclonal antibodies for detecting total Akt protein that are validated for IHC-P with mouse samples. These are as follows: 

    • Akt (pan) (C67E7) Rabbit mAb #4691
    • Akt (pa...

      Why is a fluorescent conjugate of a rabbit antibody giving no signal on compensation beads in flow cytometry?

      If you are using compensation beads in your flow cytometry experiment and observe no signal with one of our fluorescent conjugates of a rabbit antibody, this may be due to the reactivity of the compen...

      Which phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) antibody do you suggest for paraffin-embedded immunohistochemistry (IHC-P) with human samples?

      We have several antibodies for detecting p44 and p42 MAPK when phosphorylated at Thr202 and Tyr204 of Erk1, or Thr185 and Tyr187 of Erk2, that are validated for IHC-P with human samples. These are Pho...

      Is there a maximum number of cells that can be used in a single CUT&RUN assay?

      We have found 100,000 cells per assay to be sufficient for enriching target loci for multiple histone modifications, transcription factors, and transcription cofactors. However, one can increase the n...

      Which p44/42 MAPK (Erk1/2) antibody do you suggest for immunofluorescent immunocytochemistry (IF-IC) in human samples?

      We have several monoclonal antibodies for p44 and p42 mitogen-activated protein kinases (MAPKs), also referred to as Erk2 and Erk1, that are validated for IF-IC with human samples. These are p44/42 MA...

      Which cleaved PARP antibody do you suggest for immunofluorescent immunocytochemistry (IF-IC) in human samples?

      We have several antibodies for detecting cleaved PARP that are validated for IF-IC with human samples. These are Cleaved PARP (Asp214) (D64E10) XP® Rabbit mAb #5625 and Cleaved-PARP (Asp214) (E2T4K) M...

      What is the benefit of carrier vs. carrier free cytokines?

      Our cytokines and growth factors are lyophilized and supplied either with 20ug BSA per 1ug of cytokine (with carrier) or without added protein (carrier free). BSA minimizes adsorption to surfaces and ...

      Are Smad8 and Smad9 the same protein?

      Smad8 and Smad9 are often used interchangeably in the literature to refer to the same protein. However, the official name of the protein is Smad9. Moreover, there is a Smad8 in Xenopu...

      Why does the signal for LC3-II increase with chloroquine, an inhibitor of autophagy?

      The abundance of LC3-II is generally very low without treatment. Glucose starvation, amino acid starvation, serum starvation, and rapamycin treatment have been shown in the literature to induce increa...

      Where can I find the recommended primary antibody dilution buffer for western blotting (BSA or milk)?

      The recommended primary antibody dilution buffer for western blotting (i.e., BSA or nonfat dry milk) can be found either in a grey box on the bottom of the first page of the product datasheet or near ...

      Are the Protein A Magnetic Beads #73778 and the Protein G Magnetic Beads #70024 hydrophilic or hydrophobic?

      The base particle of the Protein A Magnetic Beads #73778 and the Protein G Magnetic Beads #70024 is somewhat hydrophilic. It is difficult to predict the hydrophilicity or hydrophobicity of the protein...

      What can I use as a loading control with the Cell Fractionation Kit #9038?

      Due to the nature of the Cell Fractionation Kit #9038 and the localization of each of the proteins in the Loading Control Sampler Kit #5142, the proteins will not be equally expressed in all of the fr...

      What is the difference between phospho-Smad2 (Ser465/467) and phospho-Smad2 (Ser245/250/255)?

      Phosphorylation of serines 465 and 467 indicates Smad2 activation and nuclear localization; whereas, phosphorylation of serines 245, 250, and 255 is inhibitory and leads to retention of Smad2 in the c...

      Which cleaved PARP antibody do you suggest for paraffin-embedded immunohistochemistry (IHC-P) with human samples?

      We have several antibodies for cleaved PARP that are validated for IHC-P in human samples. These are