Render Target: SSR
Render Timestamp: 2024-10-31T23:48:51.788Z
Commit: 23cb9f61fe67e1e9093fd644a533c4ff516a6463
1% for the planet logo

Technical support

Results (652)

How long after the completion of staining can I wait to image my slides when performing a SignalStar™ Multiplex IHC assay?

For Imaging Round 1, the staining should show robust signal when imaged up to 8 hours post completion of staining. For Imaging Round 2, imaging should be performed as close to the completion of staini...

What is the species reactivity of the SUMO remnant PTMScan® kits that utilize WaLP for peptide digest?

Wild type alpha-lytic protease (WaLP) is a serine endopeptidase that cleaves at the carboxyl terminal side of amino acids alanine, serine, threonine, and valine.

WaLP recognizes and cleaves SUM...

What is your protocol for PNGase F treatment?

PNGase F cell treatment:
To deglycosylate a protein of interest in native conditions.

Reagents: 
PNGase F, NEB P0704L (https://...

Are the Protein A Magnetic Beads #73778 and the Protein G Magnetic Beads #70024 hydrophilic or hydrophobic?

The base particle of the Protein A Magnetic Beads #73778 and the Protein G Magnetic Beads #70024 is somewhat hydrophilic. It is difficult to predict the hydrophilicity or hydrophobicity of the protein...

When comparing my SignalStar™ staining to the chromogenic staining on a serial section, I see more positive cells. How do I know if this excess staining is correct?

During the course of optimization, we’ve found that fluorescent staining may show higher %-positivity than chromogenic staining. To ensure any excess staining is specific, confirm that the correct sub...

How many reads per cell should I allocate for the InTraSeq™ library?

The number of reads for the InTraSeq protein library should be at least 5,000 reads per cell.

Can I autoclave the 6-Tube Magnetic Separation Rack #7017?

No, these racks cannot be autoclaved. Exposing them to high autoclave temperatures may cause the magnets to lose their magnetism. Instead, we suggest submerging or spraying the rack with 10% bleach...

Should I be concerned that my Concanavalin A beads "clump together" in some of my CUT&RUN experiments?

The Concanavalin A beads can clump together rather easily, especially when the cells become lysed. To avoid this, make sure your cells are healthy and be sure to treat them very gently during the cell...

Can my slides be used for other assays after SignalStar™ Multiplex IHC?

SignalStar™ Multiplex IHC Kits & Reagents have not been validated for use in combination with other assays. The SignalStar technology does not destroy the tissue, so it may be possible to perform ...

Why do I need to use the three buffers in the Cell Fractionation Kit #9038?

Each buffer in the Cell Fractionation Kit #9038 contains detergents to help isolate different cellular fractions. The Cytoplasmic Isolation Buffer (CIB) contains a non-ionic detergent used as a cell p...

My items say to store at -20°c but they were shipped in an envelope. Is it ok to use?

Yes, it is safe to use these items. Since 2003, CST has been shipping antibodies at room temperature when possible to reduce waste from packing materials. All CST antibodies are stability tested by ex...

Is the VersicanG1-Fc (Rabbit IgG1) #15933 product homologous with all species?

The VersicanG1-Fc (Rabbit IgG1) #15933 contained in the Hyaluronan Complete Tissue Staining Kit (HRP) #38822 and Hyaluronan Complete Tissue Staining Kit (Alexa Fluor® 488) #53721 has been tested succe...

Do you have mouse-reactive antibodies available for SignalStar™ Multiplex IHC?

Yes, please select “Mouse” in the first step of the SignalStar Multiplex IHC Panel Builder ...

Which cleaved caspase-3 (Asp175) antibody do you suggest for paraffin-embedded immunohistochemistry (IHC-P) with mouse samples?

We have several antibodies for detecting caspase-3 when cleaved at Asp175 that are validated for IHC-P with mouse samples. These are: