Technical Support Articles
Results (689)
What is the subtype composition of Normal Mouse IgG #68860?
Normal Mouse IgG #68860 is purified from pooled normal serum, making it a mixture of IgG subtypes rather than one specific subtype.
Will the Phospho-PI3 Kinase p85 (Tyr458)/p55 (Tyr199) Antibody #4228 work for human samples?
The antigen for our Phospho-PI3 Kinase p85 (Tyr458)/p55 (Tyr199) Antibody #4228 shares 100% sequence homology with human. However, we have tested many different human samples in-ho...
Can the SARS-CoV-2 serological ELISA kits assess the effectiveness of a SARS-CoV-2 vaccine using vaccine-injected human blood samples?
Yes, potentially all of our SARS-CoV-2 serological ELISA kits could be useful for studying samples from vaccine-injected subjects. Please follow The antigenic peptides used to produce our Atg4B (D1G2R) Rabbit mAb #13507, our Atg4B Antibody #5299, our Phospho-Atg4B (Ser383) Antibody #19386, and our Phospho-Atg4B (Ser383) (E3... There may be some lot-to-lot variability in the signal strength of our Vimentin (V9) Mouse mAb #49636 when testing by western blot due to this antibody being sold by weight instead... We have several antibodies for detecting caspase-3 when cleaved at Asp175 that are validated for IHC-P with mouse samples. These are:
Do your Atg4B antibodies cross-react with Atg4A, Atg4C, and Atg4D?
Are there any lot-to-lot differences when using your Vimentin (V9) Mouse mAb #49636 for western blot?
Which cleaved caspase-3 (Asp175) antibody do you suggest for paraffin-embedded immunohistochemistry (IHC-P) with mouse samples?
What is the intended use of the PTMScan® Control peptides?
These synthetic peptides are recoverable using our immunoaffinity purification (IAP) antibody beads, and thus provide users with a measure of PTM...
Why was the Caspase-3 (8G10) Rabbit mAb #9665 discontinued?
Additional internal testing has shown that the Caspase-3 (8G10) Rabbit mAb #9665 recognizes both Caspase-3 and Caspase-7. When this antibody was released to the market, we were una...
What is your protocol for treating western blot membranes with CIP?
CIP-treated membranes are prepared with the following protocol:
REAGENTS:
Quick CIP - #M0525 (New England Biolabs)
rCutSmart™ Buffer - #B6004 (New England Biolabs)...
Why do I see a doublet with your Phospho-c-Jun (Ser73) (D47G9) XP® Rabbit mAb #3270?
It is possible to see a doublet with our Phospho-c-Jun (Ser73) (D47G9) XP Rabbit mAb #3270 in some extracts. This is because #3230 can recognize JunD phosphorylated at Ser100. Phos...
How do I determine the concentration of my fractionated samples using the Cell Fractionation Kit #9038?
Since the buffers in the Cell Fractionation Kit #9038 contain SDS, you should use a commercially available ready-to-use, detergent- and reducing agent-compatible assay to quickly m...
Can DMSO be used instead of DMF in the staining protocol for the Senescence β-Galactosidase Staining Kit #9860?
X-Gal can be tricky to solubilize, therefore we recommend dissolving in DMF (...
Where can I find the recommended primary antibody dilution buffer for western blotting (BSA or milk)?
The recommended primary antibody dilution buffer for western blotting (i.e., BSA or nonfat dry milk) can be found either in a grey box on the bottom of the first page of the produc...
Must I perform an Fc receptor blocking step with the CD16/CD32 (2.4G2) Rat mAb #88280 in every immunostaining experiment?
It is difficult to predict when the Fc receptor blocking step is necessary, due to the variable interaction of host species with different antibody isotypes and the interference ca...
How is the PTMScan® HS Ubiquitin/SUMO Remnant Motif (K-ε-GG) Kit #59322 different from traditional, agarose bead PTMScan®?
The PTMScan® HS Ubiquitin/SUMO Remnant Motif (K-ε-GG) Kit #59322 uses the same antibody as in the traditional PTMScan Ubiquitin Remnant Motif (K-ε-GG) Kit #5562 and PTMScan Pilot U...
Why do I see multiple bands with your c-Myc antibodies?
Multiple bands may be observed when detecting c-Myc because there are multiple isoforms of c-Myc and c-Myc undergoes several potential post-translational modifications (e.g., phosp...
Can you help me calculate the bioactivity of my cytokine?
Specific activity (expressed in units/mg) is a function of cytokine or growth factor purity and appropriate protein folding. To determine the specific activity, typically the reado...
Do you have recommendations for p53 western blot multiplex pairs?
We have not conducted any fluorescent western blot multiplex experiments with p53. Unfortunately, many p53 antibodies have antigenic regions that overlap or have not been precisely...
How do you make the 4X RNA Denaturing Buffer for RNA dot blot?
4X RNA Denaturing Buffer Recipe for use with our RNA dot blot protocol:
657ul Formamide @99.5%
210ul of 37% Formaldehyde
133ul of 10x MOPS buffer
Final formulat...
Do the Cre Recombinase (D7L7L) XP Rabbit mAb #15036 and Cre Recombinase (D3U7F) Rabbit mAb #12830 detect Cre in transgenic mouse models?
The Cre Recombinase (D7L7L) XP Rabbit mAb #15036 has a "recombinant" sensitivity. Therefore, it can detect Cre in transgenic mouse models. In contrast, the Cre Recombinas...
Do your IRAK4 Antibody #4363 and IRAK-M Antibody #4369 cross-react with other IRAK family members?
The antigenic peptides used to produce our IRAK4 Antibody #4363 and our IRAK-M Antibody #4369 have been carefully selected to avoid cross-reactivity with the other IRAK family memb...
What is the concentration of your Digitonin Solution #16359?
The concentration of our Digitonin Solution #16359 is proprietary. The concentration will vary between lots, as we internally test each lot to match performance before release. Thi...
What epitope is recognized by your LAMP1 (D4O1S) Mouse mAb #15665?
Our LAMP1 (D4O1S) Mouse mAb #15665 was produced by immunizing animals with a recombinant protein fragment of human LAMP1 protein (see UniProt ID # P11279; https://www.uniprot.org/u...
Does your Cas9 (7A9-3A3) Mouse mAb #14697 detect the D10A/H840A mutant (dCas9) and the D10A mutant (niCas9)?
Our Cas9 (7A9-3A3) Mouse mAb #14697 was produced with a recombinant protein specific to the amino terminus of Cas9 from Streptococcus pyogenes (UniProt ID #Q99ZW2; https://w...
Can the UNC5B (D9M7Z) Rabbit mAb #13851 recognize cleaved UNC5B?
The epitope of our UNC5B (D9M7Z) Rabbit mAb #13851 is located near the caspase cleavage site (Asp412). Therefore, with extracts prepared from apoptotic cells or from high expressio...
Will the Rab5A Antibody #2143 detect Ras-related proteins Rab-5B or Rab-5C?
The antigen for our Rab5A Antibody #2143 corresponds to residues surrounding Gly190 of human Ras-related protein Rab-5A (UniProt ID P20339). The antibody is predicted to cross-reac...
Will your Anti-biotin, HRP-linked Antibody #7075 cross-react with endogenous biotin in cell lysates?
Our Anti-biotin, HRP-linked Antibody #7075 is intended for use with our Biotinylated Protein Ladder Detection Pack #7727. However, it is possible for this antibody to also detec...
Can I store my plates in PBS prior to staining with the Senescence β-Galactosidase Staining Kit #9860?
We typically perform this assay using the Senescence β-Galactosidase Staining Kit (#9860) from start to finish in one day and have not paused between fixing and staining. Therefore...
Do you have mouse-reactive antibodies available for SignalStar™ Multiplex IHC?
Yes, please select “Mouse” in the first step of the SignalStar Multiplex IH...
Do any of your PD-L1 antibodies detect mouse PD-L1 by western blot?
Our PD-L1 (D4H1Z) Rabbit mAb #60475 is approved for western immunoblot in mouse samples. We also offer our PD-L1 (D5V3B) Rabbit mAb (Mouse Specific; IHC Specific) #64988, however t...