Technical Support Articles

Results (695)

Which β-actin antibody do you suggest for immunofluorescence-immunocytochemistry (IF-IC) in human samples?

We have several antibodies for detecting β-actin that are validated for IF-IC with human samples. These include:

β-Actin (13E5) Rabbit mAb #4970

β-Actin (D6A8) Rabbit mAb #...
Why is the concentration of my antibody so low?

At CST, our antibodies are formulated on the basis of their reactivity and performance for each approved application at a recommended dilution. For antibodies with high affinity, optimal performan...
Which cleaved caspase-3 (Asp175) antibody do you suggest for paraffin-embedded immunohistochemistry (IHC-P) with mouse samples?

We have several antibodies for detecting caspase-3 when cleaved at Asp175 that are validated for IHC-P with mouse samples. These are:
- Cleaved ...
  Which cleaved caspase-3 (Asp175) antibody do you suggest for immunofluorescent immunocytochemistry (IF-IC) with human samples?
  
  We have several antibodies for detecting Cleaved Caspase-3 (Asp175) that are validated for IF-IC with human samples. These are:
  - Cleaved Caspas...
    What is an appropriate positive control to include in this SignalStar™ assay? Are multiple controls necessary?
    
    Any tissue shown to be positive for each marker via chromogenic IHC can serve as a positive control tissue for a SignalStar™ assay. Each target will therefore require a positive control, which may som...
    Can I purchase your Sample Normalization Spike-In DNA (2 pg/µl) #29987 as a stand-alone product?
    
    Unfortunately, we are unable to provide the Sample Normalization Spike-In DNA (2 pg/µl) #29987 as a stand-alone product. This product is meant for use, in tan...
    Are the bands detected by your MT1-MMP (D1E4) Rabbit mAb #13130 the precursor (62 kDa) and active (50 kDa) forms?
    
    Our MT1-MMP (D1E4) Rabbit mAb #13130 was produced with an antigenic peptide corresponding to residues surrounding Met293 of human MT1-MMP protein (see UniProt ID #P50281; https://www.uniprot.org/unipr...
    Does the α-Synuclein (D37A6) XP Rabbit mAb #4179 detect human α-Synuclein and/or high-molecular α-synuclein species?
    
    Please refer to following references for α-Synuclein:
    PMID: 27322773 (https://www.ncbi.nlm.nih.gov/pubmed/27322773) Supplemental Figure S1 C - shows detection by western blot with clone D37A6 of hi...
    What is the species reactivity of the SUMO remnant PTMScan® kits that utilize WaLP for peptide digest?
    
    Wild type alpha-lytic protease (WaLP) is a serine endopeptidase that cleaves at the carboxyl terminal side of amino acids alanine, serine, threonine, and valine.
    
    WaLP recognizes and cleaves SUM...
    Do you have mouse-reactive antibodies available for SignalStar™ Multiplex IHC?
    
    Yes, please select “Mouse” in the first step of the SignalStar Multiplex IHC Panel Builder ...
    What controls should I include when performing a Simple Western™ experiment?
    
    In addition to a known positive control sample, we recommend the following negative controls for your Simple Western™ experiment.
    
    1. A no lysate control. This includes the primary antibody at t...
    When comparing my SignalStar™ staining to the chromogenic staining on a serial section, I see more positive cells. How do I know if this excess staining is correct?
    
    During the course of optimization, we’ve found that fluorescent staining may show higher %-positivity than chromogenic staining. To ensure any excess staining is specific, confirm that the correct sub...
    Can I store the unopened, lyophilized PTMScan® LysC Protease #84748 at -80°C instead of -20°C?
    
    Yes, the lyophilized PTMScan® LysC Protease #84748 may be stored at -80°C. However, we do not recommend storing the LysC powder in a frost-free freezer or a freezer that undergoes freeze-thaw cycles. ...
    What is the extinction coefficient of my rabbit antibody?
    
    The molar extinction coefficient of rabbit IgG at 280 nm is approximately 210,000 M-1cm-1.
    Why can't spike-in DNA be used in CUT&Tag assays?
    
    Tagmentation takes place within the nuclei. To minimize non-specific background, any excess Tn5 enzyme is washed away prior to activation. Similarly, spike-in DNA introduced into the reaction woul...
    Which caspase-3 antibody do you suggest for paraffin-embedded immunohistochemistry (IHC-P) with human samples?
    
    We have two antibodies for detecting caspase-3 that are validated for IHC-P with human samples, Caspase-3 Antibody #9662 and Caspase-3 (D3R6Y) Rabbit mAb (IHC Formulated) #14214. We recommend #14214 f...
    What positive and negative controls can I use to demonstrate that my CUT&Tag experiment is successful?
    
    We recommend using a CUT&Tag-validated positive control antibody, like our Tri-Methyl-Histone H3 (Lys4) (C42D8) Rabbit mAb #9751, in your experiment to confirm that your CUT&Tag assay is worki...
    Why does the TFE3 Antibody #14779 detect multiple bands?
    
    The higher band obtained with the TFE3 Antibody #14779 at approximately 80 kDa represents the sumoylated form of the TFE3 protein, which migrates higher than the unmodified form [see Miller, A....
    How did you determine that a NG-seq sequencing depth of 3 to 5 million sequencing reads was sufficient for CUT&RUN analysis?
    
    This decision was based on our analysis of hundreds of CUT&RUN samples. We found that a sequencing depth lower than 3 million generated too few peaks and that the number of peaks increased with de...
    Will the SignalStain® DAB Substrate Kit #8059 still perform as expected if the material has been frozen?
    
    While the SignalStain® DAB Substrate Kit #8059 does have a recommended storage temperature of 4°C, we have found tha...
    Which total Akt antibody do you suggest for paraffin-embedded immunohistochemistry (IHC-P) with human samples?
    
    We have several monoclonal antibodies for detecting total Akt protein that are validated for IHC-P in human samples. These are as follows:
    - Akt (pan)...
      Which Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) antibody do you suggest for immunofluorescent immunocytochemistry (IF-IC) with mouse samples?
      
      We have several monoclonal antibodies for detecting p44 and p42 MAPK when phosphorylated at Thr202 and Tyr204 of Erk1, or Thr185 and Tyr187 of Erk2, that are validated for IF-IC with mouse samples. Th...
      What are the specifications for the 96-well plate in the CST ELISA kits?
      
      For the 96-well colorimetric PathScan® and FastScan® ELISA plates, the specification information can be found in the
      Is the “Best By” date the expiration date for the product? Will the product still work after that date?
      
      Our products can be expected to perform at optimal levels up until the printed "Best" by date on the vial when stored under the recommended conditions. Many of our products will work well af...
      Can CUT&Tag-enriched DNA be analyzed by qPCR, or do I have to do NGS to analyze my data?
      
      If qPCR analysis is desired, we recommend performing CUT&RUN, as CUT&Tag DNA is not compatible with qPCR. The sample incubation step at 58℃ required for tagmented DNA solubilization in the ...
      Which Cas9 antibody is the most sensitive by western immunoblot?
      
      The most sensitive antibody will depend on whether you are trying to detect Cas9 protein from Streptococcus pyogenes, Staphylococcus aureus, or Campylobacter jejuni. The Cas9 (7A9...
      Why does the material in my new vial of Echinomycin #51434 look different?
      
      Echinomycin #51434 is lyophilized into the vial, and any difference in the appearance of the lyophilized echinomycin is due to a dry coating of the compound in the vial. When you add dimethylsulfoxide...
      Which beta-actin antibody do you suggest for paraffin-embedded immunohistochemistry (IHC-P) with mouse samples?
      
      We have several antibodies for β-actin that are validated for IHC-P in mouse samples. These are β-Actin (13E5) Rabbit mAb #4970 and β-Actin (8H10D10) Mouse mAb #3700. We would recommend β-Actin (13E5)...
      Which p44/42 MAPK (Erk1/2) antibody do you suggest for paraffin-embedded immunohistochemistry (IHC-P) with human samples?
      
      We have several monoclonal antibodies for studying p44 and p42 mitogen-activated protein kinases (MAPKs), also referred to as Erk2 and Erk1, that are validated for IHC-P with human samples. These are ...
      Have you done any cross-reactivity testing with α-CGRP, β-CGRP and unprocessed precursors using the CGRP (D5R8F) Rabbit mAb #14959?
      
      Our CGRP (D5R8F) Rabbit mAb #14959 is produced with a synthetic peptide corresponding to residues surrounding Val114 of human CGRP protein (UniProt ID P06881 isoform 3; http://www.uniprot.org/uniprot/...

Technical Support Articles

Results (695)

Which β-actin antibody do you suggest for immunofluorescence-immunocytochemistry (IF-IC) in human samples?

Why is the concentration of my antibody so low?

Which cleaved caspase-3 (Asp175) antibody do you suggest for paraffin-embedded immunohistochemistry (IHC-P) with mouse samples?

Which cleaved caspase-3 (Asp175) antibody do you suggest for immunofluorescent immunocytochemistry (IF-IC) with human samples?

What is an appropriate positive control to include in this SignalStar™ assay? Are multiple controls necessary?

Can I purchase your Sample Normalization Spike-In DNA (2 pg/µl) #29987 as a stand-alone product?

Are the bands detected by your MT1-MMP (D1E4) Rabbit mAb #13130 the precursor (62 kDa) and active (50 kDa) forms?

Does the α-Synuclein (D37A6) XP Rabbit mAb #4179 detect human α-Synuclein and/or high-molecular α-synuclein species?

What is the species reactivity of the SUMO remnant PTMScan® kits that utilize WaLP for peptide digest?

Do you have mouse-reactive antibodies available for SignalStar™ Multiplex IHC?

What controls should I include when performing a Simple Western™ experiment?

When comparing my SignalStar™ staining to the chromogenic staining on a serial section, I see more positive cells. How do I know if this excess staining is correct?

Can I store the unopened, lyophilized PTMScan® LysC Protease #84748 at -80°C instead of -20°C?

What is the extinction coefficient of my rabbit antibody?

Why can't spike-in DNA be used in CUT&Tag assays?

Which caspase-3 antibody do you suggest for paraffin-embedded immunohistochemistry (IHC-P) with human samples?

What positive and negative controls can I use to demonstrate that my CUT&Tag experiment is successful?

Why does the TFE3 Antibody #14779 detect multiple bands?

How did you determine that a NG-seq sequencing depth of 3 to 5 million sequencing reads was sufficient for CUT&RUN analysis?

Will the SignalStain® DAB Substrate Kit #8059 still perform as expected if the material has been frozen?

Which total Akt antibody do you suggest for paraffin-embedded immunohistochemistry (IHC-P) with human samples?

Which Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) antibody do you suggest for immunofluorescent immunocytochemistry (IF-IC) with mouse samples?

What are the specifications for the 96-well plate in the CST ELISA kits?

Is the “Best By” date the expiration date for the product? Will the product still work after that date?

Can CUT&Tag-enriched DNA be analyzed by qPCR, or do I have to do NGS to analyze my data?

Which Cas9 antibody is the most sensitive by western immunoblot?

Why does the material in my new vial of Echinomycin #51434 look different?

Which beta-actin antibody do you suggest for paraffin-embedded immunohistochemistry (IHC-P) with mouse samples?

Which p44/42 MAPK (Erk1/2) antibody do you suggest for paraffin-embedded immunohistochemistry (IHC-P) with human samples?

Have you done any cross-reactivity testing with α-CGRP, β-CGRP and unprocessed precursors using the CGRP (D5R8F) Rabbit mAb #14959?