Technical Support Articles
Results (689)
What controls should I include when performing a Simple Western™ experiment?
In addition to a known positive control sample, we recommend the following negative controls for your Simple Western™ experiment.
1. A no lysate control. This includes the p...
Is it possible to perform CUT&Tag on frozen tissue samples?
Yes, our CUT&Tag Assay Kit works with flash frozen tissue samples or tissue samples directly frozen at -80℃. After thawing, fixation of the tissue sample is optional, as we hav...
Does your Tau (D1M9X) XP® Rabbit mAb #46687 recognize all six Tau isoforms?
Our Tau (D1M9X) XP® Rabbit mAb #46687 is predicted to recognize 2N4R (tau40), 2N3R (tau39), 1N4R (tau46), 1N3R (tau37), 0N4R (tau24), and 0N3R (tau23), as all six isoforms include ...
Why do I see high background by western blot when probing for phospho-AMPK (Thr172)?
If CST recommended buffers are being used for western blotting and high background or additional bands persist when probing for phospho-AMPK (Thr172), the issue could be related to...
Can I use the Cyclic AMP XP® Assay Kit #4339 with tissue samples?
We have not tested tissue samples in-house using the Cyclic AMP XP Assay Kit #4339, so although we cannot guarantee it will work, there is no reason it should not. We recommend loa...
How do I validate the PTM sites identified in PTMScan® experiments?
The validation of PTM sites may be accomplished in several ways. Often, the site of interest may be seen in the context of more than one peptide due to alternative forms caused by ...
Why was the Caspase-3 (8G10) Rabbit mAb #9665 discontinued?
Additional internal testing has shown that the Caspase-3 (8G10) Rabbit mAb #9665 recognizes both Caspase-3 and Caspase-7. When this antibody was released to the market, we were una...
Does your Insulin (C27C9) Rabbit mAb #3014 detect proinsulin?
Our Insulin (C27C9) Rabbit mAb #3014 was produced with an antigenic peptide corresponding to residues within the insulin B chain of the human insulin protein (UniProt ID #P01308; h...
Can I use your Protein A Agarose Beads #9863 and Protein G Agarose Beads #37478 if they have been frozen?
Although we are unable to guarantee that both our Protein A Agarose Beads #9863 and Protein G Agarose Beads #37478 will work after being stored at -20C, we have used beads that hav...
Do you have a recommended protocol for stripping/re-probing blots?
The western blot stripping/re-probing protocol for our Western Blot Stripping Buffer (5X) #91925 is below. However, it is important to note that we typically advise against strippi...
Why should I include a stage tip step in my PTMScan® experiment?
For PTMScan experiments, the stage tip, or small-scale peptide desalting is a critical part of the experiment for a number of reasons. Detailed instructions for performing stage ti...
Is PTMScan® compatible with SILAC?
PTMScan® is compatible with the use of stable isotope labeling using amino acids in cell culture, or SILAC. The incorporation of heavy stable iso...
Which cleaved caspase-3 (Asp175) antibody do you suggest for immunofluorescent immunocytochemistry (IF-IC) with mouse samples?
We have several antibodies for detecting caspase-3 when cleaved at Asp175 that are validated for IF-IC with mouse samples. These are:
- Cleaved Caspase-3 (Asp175) Antibody #9...
What is the difference between your SimpleChIP® ChIP-seq Single Index Primers #29580 and SimpleChIP® ChIP-seq Dual Index Primers #47538?
Both SimpleChIP® ChIP-seq Multiplex Oligos for Illumina® (Single Index Primers) #29580 and SimpleChIP® ChIP-seq Multiplex Oli...
Can the Cell Fractionation Kit #9038 be used for frozen cell pellets?
We have had customers perform this assay using frozen cell pellets, prior to adding any fractionation buffer, and the subsequent fractionation worked fine. Once you start fracti...
What is the best way to choose an antibody for my CUT&RUN assay if I cannot find a CUT&RUN validated antibody for my target protein?
When selecting an antibody for use in CUT&RUN, we recommend starting with a ChIP or ChIP-seq-validated antibody; however, we have found that not all ChIP-validated antibodie...
What are the high molecular weight bands detected by the β1-Adrenergic Receptor Antibody #12271?
The antigen for the β1-Adrenergic Receptor Antibody #12271 surrounds Gly406 of the human β1-Adrenergic Receptor protein and is contained entirely in the cytoplasmic domain. #12271 ...
How long after the completion of staining can I wait to image my slides when performing a SignalStar™ Multiplex IHC assay?
For Imaging Round 1, the staining should show robust signal when imaged up to 8 hours post completion of staining. For Imaging Round 2, imaging should be performed as close to the ...
Can I use RIPA Buffer (10X) #9806 for ELISA experiments?
Our ELISA kits are compatible with our RIPA Buffer (10X) #9806, however, this bu...
Can I autoclave the 6-Tube Magnetic Separation Rack #7017?
No, these racks cannot be autoclaved. Exposing them to high autoclave temperatures may cause the magnets to lose their magnetism. Instead, we suggest submerging or spraying the ...
Can you provide data that confirms the specificity of your BACE (D10E5) Rabbit mAb #5606?
The specificity of our BACE (D10E5) Rabbit mAb #5606 has been confirmed in the literature on brain extracts prepared from BACE1 (-/-) mice. Please see Figure 1 in Shahani, N. et al...
Can my slides be used for other assays after SignalStar™ Multiplex IHC?
SignalStar™ Multiplex IHC Kits & Reagents have not been validated for use in combination with other assays. The SignalStar technology does not destroy the tissue, so it may be ...
Which caspase-3 antibody do you suggest for paraffin-embedded immunohistochemistry (IHC-P) with human samples?
We have two antibodies for detecting caspase-3 that are validated for IHC-P with human samples, Caspase-3 Antibody #9662 and Caspase-3 (D3R6Y) Rabbit mAb (IHC Formulated) #14214. W...
What are the differences between the BrdU Cell Proliferation Chemiluminescent Assay Kit #5492 and the BrdU Cell Proliferation Assay Kit #6813? Which should I choose?
In general, the BrdU Cell Proliferation Chemiluminescent Assay Kit #5492 offers a broader dynamic range and higher sensitivity compared to the BrdU Cell Proliferation Assay Kit #68...
What is the expected size distribution of CUT&RUN DNA?
When using antibodies for CUT&RUN against histone modifications, we typically see fragment sizes as small as 150 bp (size of a mono-nucleosome) and quite often see a nucleosoma...
How much CUT&RUN DNA is required for NG-seq analysis?
The amount of CUT&RUN DNA required for NG-seq analysis depends on the sensitivity of the DNA library prep kit. The typical yield for CUT&RUN DNA is 0.6 to 6 ng per reaction...
What samples and species were tested with the Griess Reagent Nitrite Measurement Kit #13547?
We have tested the Griess Reagent Nitrite Measurement Kit #13547 with media, cell culture supernatant, and urine. It should cross-react with all species types.
Which total Akt antibody do you suggest for paraffin-embedded immunohistochemistry (IHC-P) with human samples?
We have several monoclonal antibodies for detecting total Akt protein that are validated for IHC-P in human samples. These are as follows:
- Akt (pan) (C67E7) Rabbit mAb #46...
Can I use mixed species samples for a PTMScan® experiment?
The PTMScan® technology is compatible with samples derived from all kingdoms of life as well as samples containing mixed species like xenografts (mouse and human), microbiome sampl...
What DNA library prep kit do you recommend using for CUT&RUN?
We recommend using our SimpleChIP® ChIP-seq DNA Library Prep Kit for Illum...