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How to Incorporate Cell Pellet Controls in IHC Experiments


When setting up immunohistochemistry (IHC) experiments, cell pellet controls can tell you whether the protocol is working, or needs to be adjusted. You need to confirm that your antibody is detecting the intended target, and not something else. Tissue controls can be used for this purpose, but when it's not known which tissue to select as positive and negative controls, cell pellets with known expression of the protein of interest can be valuable.