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Top 5 Frequently Asked CUT&RUN Questions


CUT&RUN is a low cell alternative to chromatin immunoprecipitation or ChIP assays, used to profile protein-DNA interactions. This video provides answers to the top five frequently asked CUT&RUN questions:

  • What antibody validation data should I expect for CUT&RUN, and what should I do if I can’t find a CUT&RUN validated antibody for my target?
  • What controls should I use for my CUT&RUN experiment?
  • What do I do if my beads are clumping together?
  • What do I do if there isn’t enough DNA to quantitate before library construction and NGS?
  • Should I use phenol-chloroform extraction or spin columns to purify the DNA from CUT&RUN?

For answers to other frequently asked CUT&RUN questions, please visit the full FAQ page, or to learn about the benefits of CUT&RUN, visit the CUT&RUN application page.